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Diss Factsheets
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EC number: 228-602-0 | CAS number: 6303-30-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling schedule:
Control : at 48 hours (on 2020-11-05)
Test concentration/s : 25, 50 and 100 mg/L in the freshly prepared media (on 2020-11-03 and 2020-11-04) and in the media after 24 h of exposure (on 2020-11-04 and
2020-11-05) according to the semi-static
Pre-treatment of test item and preparation of test item concentrations
A stock solution was prepared to give the desired series of test concentrations. To achieve this 100.5 mg of the test item on 2020-11-02 and 101.0 mg on 2018-11-03 were added to 1 litre of dilution water, treated for 60 seconds at 8000 rpm with an ultra turrax and stirred for 24 h on a magnetic stirrer. Undissolved particles of the test item were removed by filtration using a folded filter with a pore size of 7 - 12. The pH was measured to be 7.4 on 2020-11-03 and to be 7.5 on 2020-11-04.
To produce the different test item concentrations appropriate amounts of the stock solution were diluted with dilution water to a volume of 500 mL. 50 mL of the prepared solutions were taken per replicate and 0.5 mL of dilution water containing 5 daphnids was given to all replicates resulting in the final nominal concentrations. For each test item exposure concentration and for the control exposure 4 replicates were prepared. After 24 hours of exposure, all media were renewed, and the neonates were transferred into the fresh media using a small sieve. - Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test species
Name : Daphnia magna STRAUS, parthenogenetic females
Source : Strain of Bundesgesundheitsamt Berlin
Maintenance and Acclimatisation : A population of parthenogenetic females of synchronized age structure has been maintained for more than 20 years in the test facility under constant temperature conditions (20 +/- 2 °C) at a 16 : 8 hour light : dark photoperiod (light intensity: < 20 µE x m-2 x s-1). The culture water (so-called 'M4 medium') was partly renewed once a week. The Daphnia were exclusively fed unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period were recorded daily. The neonates were separated from their parent Daphnia by filtration prior to the acute test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 d
- Hardness:
- 14.4 °dH (= 257 mg/L CaCO3)
- Test temperature:
- 18 - 22 °C
- pH:
- 7.4 - 7.5
- Nominal and measured concentrations:
- 25, 50 and 100 mg/L (nominal)
- Reference substance (positive control):
- yes
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates
- Validity criteria fulfilled:
- yes
- Conclusions:
- No toxic effects against Daphnia were observed at a limit test concentration of 100 mg/L under exposure conditions.
- Executive summary:
A study was performed to assess the acute toxicity of Diisobutyl phosphate to Daphnia magna STRAUS under semi-static conditions in a closed bottle system.
The study was conducted in accordance with the Council Regulation (EC) No 440/2008, Method C.2 ‘Acute toxicity for Daphnia’ (2008) which is equivalent to OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test' (adopted April 13, 2004).
The Daphnia were exposed to a range of concentrations, nominally 25, 50 and
100 mg/L of Diisobutyl phosphate dissolved in dilution water.
Observations were made on the swimming ability and the percentage immobilised, respectively, after 24 and 48 hours of exposure. The following values were determined:
Time EC 50
[h] [mg/L]24 > 100
48 > 100
No toxic effects against Daphnia were observed at a limit test concentration of 100 mg/L under exposure conditions.
The results are expressed in terms of nominal concentrations. Measured test item exposure concentrations ranged from 92 % to 97 % of their nominal target values in the freshly prepared media and from 82 % to 103 % in the media after 24 hours of exposure.
Reference
Description of key information
No toxic effects against Daphnia were observed at a limit test concentration of 100 mg/L under exposure conditions.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 100 mg/L
Additional information
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