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EC number: 203-728-9 | CAS number: 110-01-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Tetrahydrothiophene
- EC Number:
- 203-728-9
- EC Name:
- Tetrahydrothiophene
- Cas Number:
- 110-01-0
- Molecular formula:
- C4H8S
- IUPAC Name:
- tetrahydrothiophene
- Details on test material:
- Source: Pennwalt Corporation
Batch No.: Lot 271.
Purity: 99.0% minimum.
Constituent 1
Method
- Target gene:
- Histidine reversion
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver S9 from rat induced with a single ip injection of Aroclor 1254 (500 mg/kg)
- Test concentrations with justification for top dose:
- 0, 50, 150, 500, 1500, 5000 µg/plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: With S-9 mix : 2-Aminoanthracene, all strains. Without S-9 mix: 2-Nitrofluorene, TA 98; 9-Aminoacridine, TA 1537; N-ethyl-N'-nitro-N-nitrosoguanidine, TA 1535 and TA 100.
- Details on test system and experimental conditions:
- Bacterial strains:
The strains are tested routinely for cell membrane permeability and where applicable for ampicillin resistance.
For use in tests sub-cultures are grown in Nutrient Broth (Oxoid) at 37°C for 18 hours. This culture provides approximately 2 x 10e9 organisms per ml which is assessed by cell counting.
Preliminary toxicity test:
The following procedure is carried out on each bacterial strain:
Four concentrations of test substance are assessed for toxicity using the four tester strains. The highest concentration is usually 0.05 g of test substance dissolved in 1 ml of solvent. Three 10-fold serial dilutions of the top concentration are also tested. The chosen solvent is used as the negative control.
0.1 ml of an overnight bacterial culture containing approximately 2 x 10e9 cells/ml, and 0.5 ml S-9 mix or 0.5 ml 0.1 M sodium phosphate buffer (pH 7.4) are placed in glass bijou bottles. 0.1 ml of the test solution is added followed by 2 ml histidine deficient agar. The mixture is thoroughly shaken and overlaid onto previously prepared plates containing 20 ml minimal agar. Single petri dishes are used for each dose level. They are incubated at 37°C for 72 hours. After this period the plates are examined for the appearance of a complete bacterial lawn. Revertant colonies are counted using a Biotran Automatic Colony Counter. Any toxic effects of the test substance are detected by a substantial reduction in revertant colony counts or by the absence of a complete background bacterial lawn.
Ames test procedure:
- Without metabolic activation
The following procedure is carried out on each tester strain.
0.1 ml aliquots of bacterial suspension and 0.5 ml of sterile 0.1 M sodium phosphate buffer (pH 7.4) are added to each of one set of sterile bijou bottles.
0.1 ml of the test compound is added to cultures at five concentrations separated by half-log 10 intervals. The negative control is the chosen solvent. The appropriate positive control is also included. 3 bottles are used at each dose level.
2.0 ml of histidine deficient agar is added to each of the bottles, thoroughly mixed and then overlaid onto previously prepared plates containing 20 ml of minimal agar. Plates are incubated for 72 hours at 37°C.
Colonies are counted using a Biotran Automatic Colony Counter, and the mean number of revertant colonies per treatment group assessed.
- With metabolic activation
Methodology is as described previously except that 0.5 ml of liver homogenate S-9 mix is added to bijou bottles in place of sterile buffer.
Second mutation test :
The procedure outlined previously is repeated at a later date; though the concentrations of test substance used in the second test may be altered, if the results of the first test indicate this may be expedient. - Evaluation criteria:
- The mean number of revertant colonies for all treatment groups is compared with those obtained for negative and positive control groups. The effect of metabolic activation is assessed by comparing the results obtained both in the presence and absence of the liver microsomal fraction for each treatment group.
A compound is deemed to provide evidence of mutagenic potential if (1) a statistically significant dose-related increase in the number of revertant colonies is obtained in two separate experiments and (2) the increase in the number of revertant colonies is at least twice the concurrent solvent control value.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- No substantial increases in revertant colony numbers of any of the four tester strains were observed following treatment with THT at any dose level, either in the presence or absence of metabolic activation (S-9 mix).
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Mutation Test 1 (mean values)
Strain |
TA 1535 |
TA 1537 |
TA 98 |
TA 100 |
||||
Metabolic activation |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
Dose level, µg/plate |
||||||||
5000 |
18 |
15 |
7 |
20 |
21 |
21 |
83 |
88 |
1500 |
15 |
10 |
7 |
15 |
25 |
19 |
99 |
100 |
500 |
14 |
13 |
8 |
15 |
30 |
24 |
100 |
120 |
150 |
16 |
15 |
7 |
12 |
27 |
24 |
123 |
130 |
50 |
20 |
11 |
11 |
17 |
29 |
28 |
112 |
133 |
Solvent |
12 |
13 |
10 |
16 |
36 |
22 |
129 |
132 |
Mutation Test 2 (mean values)
Strain |
TA 1535 |
TA 1537 |
TA 98 |
TA 100 |
||||
Metabolic activation |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
Doselevel,µg/plate |
||||||||
5000 |
12 |
10 |
12 |
16 |
34 |
22 |
73 |
69 |
1500 |
16 |
10 |
12 |
14 |
25 |
26 |
84 |
97 |
500 |
14 |
12 |
11 |
13 |
22 |
19 |
77 |
94 |
150 |
15 |
11 |
11 |
10 |
21 |
23 |
93 |
103 |
50 |
12 |
10 |
12 |
15 |
22 |
21 |
80 |
87 |
Solvent |
16 |
14 |
14 |
13 |
22 |
22 |
89 |
120 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Tetrahydrothiophene was concluded to be negative in the Bacterial Reverse Mutation Assay. - Executive summary:
In an OECD 471 bacterial reverse mutation test, tetrahydrothiophene, was tested in the Bacterial Reverse Mutation Assay using Salmonella typhimurium tester strains TA98, TA100, TA1535 and TA1537 in the presence and absence of Aroclor-induced rat liver S9. The assay was performed using the plate incorporation method. The dose levels tested were 50, 150, 500, 1500 and 5000 µg per plate in the initial mutation assay and in the repeated assay. No positive mutagenic response was observed. Neither precipitate nor appreciable toxicity was observed. Tetrahydrothiophene
was concluded to be negative in the Bacterial Reverse Mutation Assay.
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