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EC number: 281-889-4 | CAS number: 84057-71-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the OECD test guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- The analytical determinations were performed by UV-VIS spectrophotometer. The pre-treated stock solution was then diluted with media in order to get the required test solutions ranging from concentrations 1.25, 2.5, 5, 25, 50, 75, 100, 125, 135, 150 mg/L. The absorbance of resulting solution was measured using UV-VIS spectrophotometer against corresponding blank at lambda max (λmax). Standard curve was plotted against concentration verses absorbance and the maximum solubility was determined from the below standard curve. Analytical assessments were performed for selected test concentration at 0 hour and 96 hours. The concentration of the test chemical being tested has been satisfactorily maintained within ± 20 % of the nominal concentration throughout the test. Therefore, the analysis of the results was based on nominal concentration.
- Vehicle:
- no
- Details on test solutions:
- The test chemical was prepared by dissolving 1500 mg of test chemical in 1500 ml of RO water to get the final concentration of 1000 mg/L which was then analytically determined. The remaining test solutions were prepared by dilution from the above stock solution. Test chemical concentrations used for the study were 0, 11.25, 20.25, 36.45, 65.61, 118.09 mg/l, respectively.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra Fish
- Length at study initiation (length definition, mean, range and SD): average 0.21 cm
- Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
- Duration of housing: 15 days
- Photoperiod: 16 hour light and 8 hour darkness
- Diet : Standard brand feed, once daily
- Acclimatization: seven days
- Feeding frequency: Once in a day
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 21-25°C
- pH:
- Control:
at 0 hour: 7.8
at 96 hour: 7.6 - Dissolved oxygen:
- Control:
at 0 hour: 7.9 mg/l
at 96 hour: 5.9 mg/l - Nominal and measured concentrations:
- Test chemical concentrations used for the study were 0, 11.25, 20.25, 36.45, 65.61, 118.09 mg/l, respectively.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 7 liters of plastic aquaria filled with 4000 ml of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8-hour darkness
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 118.09 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Table: Assessment of test concentrations
Sr. no.
Concentrations (mg/l)
Wavelength (nm)
Absorbance
Temperature (°C)
1
blank
523
0.00
25
2
1.25
523
0.01
25
3
2.50
523
0.03
25
4
5.00
523
0.05
25
5
25
523
0.03
25
6
50
523
0.69
25
7
75
523
1.01
25
8
100
523
1.37
25
9
125
523
1.68
25
10
135
523
1.78
25
11
150
523
1.99
25
The absorbance and concentrations were recorded at 523 nm.
Table: Concentration after analytical Determination
Sr. No
Concentrations (mg/L)
Absorbance
(mean) (0 hour)
Analytical
Concentrations (0 hour)
Absorbance
(mean) (96 hour)
Analytical
Concentrations (96 hour)
1
blank
0.00
0.00
0.00
0.00
2
200 mg/l
0.56
189.3
0.58
195.42
3
36.45
0.50
37.51
0.51
38.70
4
65.61
0.84
62.86
0.94
70.22
5
118.09
1.57
117.5
1.62
121.35
Table: Mortality and visible symptoms
Sr. No
Test
concentrations(mg/L)
3 hr
6 hr
24 hr
48 hr
72 hr
96 hr
Cumulative
Mortality
Percent
Inhibition
1
Control
-
-
-
-
-
-
-
-
2
11.25
-
-
-
-
-
-
-
-
3
20.25
-
-
-
-
-
-
-
-
4
36.45
-
-
-
-
-
-
-
-
5
65.61
-
-
-
-
-
-
-
-
6
118.09
-
-
-
-
-
-
-
-
Sr. No
Test
concentrations(mg/L)
3 hr
6 hr
24 hr
48 hr
72 hr
96 hr
1
Control
NS8SC8
NS8SC8
NS8SC8
NS8SC8
NS8SC8
NS8SC8
2
11.25
NS8SC8
NS8SC8
NS8SC8
NS8SC8
NS8SC8
NS8SC8
3
20.25
NS8SC8
NS8SC8
NS8SC8
NS8SC8
NS8SC8
NS8SC8
4
36.45
NS8SC8
NS8SC8
NS8SC8
NS8SC8
NS8SC8
NS8SC8
5
65.61
NS8SC8
NS8SC8
NS8SC8
NS8SC8
NS8SC8
NS8SC8
6
118.09
NS8SC8
NS8SC8
NS8SC8
NS8SC8
NS8SC8
NS8SC8
Table: pH, Temperature and dissolve oxygen
Test
Concentration (mg/L)
pH
24 Hours
48 Hours
72 Hours
96 Hours
Control
7.7
7.2
7.6
7.6
11.25
7.3
7.4
7.2
7.4
20.25
7.3
7.2
7.1
7.2
36.45
7.1
7.0
7.1
7.1
65.61
6.9
7.2
7.2
7.1
118.09
7.2
7.1
7.0
7.0
Test
Concentration (mg/L)
Temperature °C
24 Hours
48 Hours
72 Hours
96 Hours
Control
21.3
20.2
21.3
21.1
11.25
21.6
20.9
21.3
21.6
20.25
21.6
20.4
21.6
21.6
36.45
21.6
20.4
21.6
21.6
65.61
21.6
20.1
21.6
21.6
118.09
21.6
20.1
21.5
21.6
Test
Concentration (mg/L)
Dissolved Oxygen
24 Hours
48 Hours
72 Hours
96 Hours
Control
6.9
5.2
5.1
5.9
11.25
4.5
5.6
4.5
5.0
20.25
6.0
5.4
4.3
4.0
36.45
4.5
4.1
4.4
4.0
65.61
4.1
4.3
3.7
3.8
118.09
5.0
5.9
5.1
3.4
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, the median lethal concentration [LC50 (96 h)] for test chemical on Danio rerio (Zebra Fish) was determined to be > 118.09 mg/L.
- Executive summary:
An acute toxicity test was conducted for 96 hrs for assessing the effect of test chemical on Zebra fish (Danio rerio). The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average length of 0.21 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 6.9 mg/l, pH 7.4, water temperature 20.7°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. The test chemical was prepared by dissolving 1500 mg of test chemical in 1500 ml of RO water to get the final concentration of 1000 mg/L which was then analytically determined by UV-VIS spectrophotometer. The remaining test solutions were prepared by dilution from the above stock solution. Test chemical concentrations used for the study were 0, 11.25, 20.25, 36.45, 65.61, 118.09 mg/l, respectively. Total 8 fishes were exposed to test chemical in a 7 lit bowl aquaria containing 4000 ml of potable water. The test vessels were placed in a room at a temperature of 21 -25°C, pH of control at 0 and 96 hour was 7.8 & 76. and DO of control at 0 and 96 hour was 7.9 & 5.9 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control and test vessel. The dissolved oxygen concentration remained above 60% of the air saturation value throughout the exposure period. Thus, fulfilling the validity criterion. As the concentration of the test chemical being tested has been satisfactorily maintained within ± 20 % of the nominal concentration throughout the test. Therefore, the analysis of the results was based on nominal concentration. On the basis of effect of test chemical on mortality of the test organism, the median lethal concentration [LC50 (96 h)] for test chemical on Danio rerio (Zebra Fish) was determined to be > 118.09 mg/L. Thus, test chemical was considered as non-toxic to aquatic fishes and hence, considered to be 'not classified' as per the CLP classification criteria.
Reference
Description of key information
An acute toxicity test was conducted for 96 hrs for assessing the effect of test chemical on Zebra fish (Danio rerio) (Experimental study report, 2019). The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average length of 0.21 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 6.9 mg/l, pH 7.4, water temperature 20.7°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. The test chemical was prepared by dissolving 1500 mg of test chemical in 1500 ml of RO water to get the final concentration of 1000 mg/L which was then analytically determined by UV-VIS spectrophotometer. The remaining test solutions were prepared by dilution from the above stock solution. Test chemical concentrations used for the study were 0, 11.25, 20.25, 36.45, 65.61, 118.09 mg/l, respectively. Total 8 fishes were exposed to test chemical in a 7 lit bowl aquaria containing 4000 ml of potable water. The test vessels were placed in a room at a temperature of 21 -25°C, pH of control at 0 and 96 hour was 7.8 & 76. and DO of control at 0 and 96 hour was 7.9 & 5.9 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control and test vessel. The dissolved oxygen concentration remained above 60% of the air saturation value throughout the exposure period. Thus, fulfilling the validity criterion. As the concentration of the test chemical being tested has been satisfactorily maintained within ± 20 % of the nominal concentration throughout the test. Therefore, the analysis of the results was based on nominal concentration. On the basis of effect of test chemical on mortality of the test organism, the median lethal concentration [LC50 (96 h)] for test chemical on Danio rerio (Zebra Fish) was determined to be > 118.09 mg/L. Thus, test chemical was considered as non-toxic to aquatic fishes and hence, considered to be 'not classified' as per the CLP classification criteria.
Key value for chemical safety assessment
Additional information
Experimental study of the test chemical and various supporting weight of evidence studies for its structurally and functionally similar read across chemical were reviewed for short term toxicity to fish end point which are summarized as below:
In an experimental study from study report (2019),an acute toxicity test was conducted for 96 hrs for assessing the effect of test chemical on Zebra fish (Danio rerio). The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average length of 0.21 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 6.9 mg/l, pH 7.4, water temperature 20.7°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. The test chemical was prepared by dissolving 1500 mg of test chemical in 1500 ml of RO water to get the final concentration of 1000 mg/L which was then analytically determined by UV-VIS spectrophotometer. The remaining test solutions were prepared by dilution from the above stock solution. Test chemical concentrations used for the study were 0, 11.25, 20.25, 36.45, 65.61, 118.09 mg/l, respectively. Total 8 fishes were exposed to test chemical in a 7 lit bowl aquaria containing 4000 ml of potable water. The test vessels were placed in a room at a temperature of 21 -25°C, pH of control at 0 and 96 hour was 7.8 & 76. and DO of control at 0 and 96 hour was 7.9 & 5.9 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control and test vessel. The dissolved oxygen concentration remained above 60% of the air saturation value throughout the exposure period. Thus, fulfilling the validity criterion. As the concentration of the test chemical being tested has been satisfactorily maintained within ± 20 % of the nominal concentration throughout the test. Therefore, the analysis of the results was based on nominal concentration. On the basis of effect of test chemical on mortality of the test organism, the median lethal concentration [LC50 (96 h)] for test chemical on Danio rerio (Zebra Fish) was determined to be > 118.09 mg/L. Thus, test chemical was considered as non-toxic to aquatic fishes and hence, considered to be 'not classified' as per the CLP classification criteria.
Another acute toxicity study was conducted for 96 hrs for assessing the effect of test chemical on fish (Experimental study report, 2018). The test was performed following the OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.066 g and average length of 1.7 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.0 mg/l, pH 7.4, water temperature 24°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. Test concentrations selected for the study was were 0, 6.25, 12.5, 25, 50 and 100 mg/L, respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 24°C, pH 7.2, hardness of water 150.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control and test vessel. On the basis of effect of test chemical on mortality of the test organism, the median lethal concentration (LC50 (96 h)) value was determined to be > 100 mg/l. Thus, test chemical was considered as non-toxic to aquatic fishes and hence, considered to be not classified as per the CLP classification criteria.
For the test chemical from peer reviewed journal and secondary sources, short term fish toxicity was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance with procedures described in the Fish-Pesticide Acute Toxicity Test Method prepared by the Animal Biology Section of the Pesticides Regulation Division of the USDA. Salmo gairdneri (rainbow trout) obtained from a commercial fish hatchery in New Jersey of average weight 0.9 g and average length of 42 mm was used as a test organism for the study. During the acclimation period (10 days), mortality was < 1% and the fish were judged to be in excellent physical condition. Fish were conditioned to test water for at least 24 hours prior to testing. Total 4 test chemical concentrations were taken. Exact test chemical conc. was not known. Test solutions were prepared by adding appropriate amounts of test chemical (mixed in 500 ml of test water) to vessels containing 14.5 liters of test water. The test chemical appeared to be in solution at all concentrations tested. Study was performed using fishes in a static system at 13 ± 0.5°C temperature and 7.1 pH. Total 40 fishes (10 fish/conc.) were exposed to test chemical in a gallon glass vessel for 96 hr. At the end of the 96 hour test period, 1-liter water samples were taken from each vessel and analyzed for concentration of test chemical. Test water consisted of 15 liters of deionized water (at least 1 million ohms resistivity) that was reconstituted by adding 3 mg KCl, 30 mg CaSO4, 30 mg MgSO4, alkalinity 35 mg/l and 48 mg NaHCO3 per liter. Fish were identified according to concentration tested and length of survival and analyzed for bioaccumulation. On the basis of effect on mortality of the test organism Salmo gairdneri (rainbow trout), the 96 hr LC50 was determined to be 750 mg/l. Thus, based on this value, test chemical was considered as non-toxic to fish and hence, considered to be ‘not classified’ as per the CLP classification criteria.
On the basis of the above results, it can be concluded that the test chemical was considered as non-toxic to fishes and hence, considered to be ‘not classified’ as per the CLP classification criteria.
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