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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study (OECD), validity criteria fulfilled

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report Date:
2002

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
TS-Freetext:
ChiPros (R)-Mandelsäure: (R)-mandelic acid (purity: 100 %)

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- original source: IRCHA Institut National de Recherche Chimique Appliquée, France, 1978
- age: 2-24 h (starting with the 3rd breed of parent animals)
- no feeding during test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
- total hardness: 2.20-3.20 mmol/l
- alkalinity to pH 4.3: 0.80-1.00 mmol/l
- molar ratio Ca:Mg: ca. 4:1
- conductivity: 550-650 µS/cm

Test temperature:
18-22 °C (max. diff. 2 °C)
pH:
7.5-8.5
Nominal and measured concentrations:
test concentrations: 0 (control), 12.5, 25, 50, and 100 mg/l
Details on test conditions:
- test vessels: glass tubes with flat bottom (20 ml)
- test volume: 10 ml
- loading: 0.5 animals/ml
- 20 animals per concentration level

- illumination: artificial light, type warm white (OSRAM L58 W31)
- day/night rhythm: 16/8 h

- observation times: visually after 0, 24 and 48 h
- observation parameters: swimming ability

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L

Any other information on results incl. tables

RS-Freetext:
Effect values (mg/l):
24 h 48 h
EC 0: >= 100 >= 100

EC 50: > 100 > 100

EC 100: > 100 > 100


Immobility:
- all test animals remained mobile throughout the test


Test concentration (retrieval, %):
time minimum maximum
(h)
0 106 114
48 106 106


Test solutions
test conc. pH dissolved oxygen (mg/l)
(mg/l) 0 h 48 h 0 h 48 h
control (0) 8.2 8.1 8.8 8.7
12.5 8.1 8.1 8.7 8.6
25 8.1 8.1 8.7 8.5
50 7.2 8.0 8.6 8.5
100 6.5 7.9 8.6 8.4

minimum and maximum values measured in separate vessels (placed close to test vessels):
- pH value : 6.5-8.2
- dissolved oxygen (mg/l): 8.4-8.8
- temperature (°C) : 19.1-19.8

Test medium (M4):
pH value: 8.3
alkalinity up to pH 4.3: 0.88 mmol/l
total hardness: 2.48 mmol/l
conductivity: 621 µS/cm

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In the control the immobilization was ≤ 10%. In the control no daphnids were captured in a surface film. The O2-concentration was > 3 mg/l in all tested replicates. The EC50(24h) of the control substance potassium dichromate (non-GLP) was 1,08 mg/l (Date of the last control experiment: 08 July 2002 project number: 02/0106/50/6). The test is valid.