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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Acceptable study report well documented that meets basic scientific principles, comparable to guideline OECD 471 (Bacterial Reverse Mutation Assay).

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
1-Amino-1-cyclopropanecarbonitrile hydrochloride
EC Number:
629-718-8
Cas Number:
127946-77-4
Molecular formula:
C4H7N2Cl
IUPAC Name:
1-Amino-1-cyclopropanecarbonitrile hydrochloride
Constituent 2
Reference substance name:
1-Cyanocyclopropanaminium
IUPAC Name:
1-Cyanocyclopropanaminium
Constituent 3
Reference substance name:
1-aminocyclopropane-1-carbonitrile hydrochloride
IUPAC Name:
1-aminocyclopropane-1-carbonitrile hydrochloride

Method

Target gene:
Target gene S.Typhimurium
Target gen E. Coli
Species / strainopen allclose all
Species / strain / cell type:
S. typhimurium TA 100
Additional strain / cell type characteristics:
not specified
Species / strain / cell type:
S. typhimurium TA 1535
Additional strain / cell type characteristics:
not specified
Species / strain / cell type:
S. typhimurium TA 97
Additional strain / cell type characteristics:
not specified
Species / strain / cell type:
S. typhimurium TA 98
Species / strain / cell type:
E. coli WP2 uvr A pKM 101
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
Liver microsomal enzymes from rats pretreated with xenobiotics.
Test concentrations with justification for top dose:
The concentration 0, 100, 300, 1000, 3000 and 6000µg/plate were chosen for the main experiments.
Vehicle / solvent:
Vehicle used: distilled water.
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
other: 2-Aminoanthracene (2AA)

Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium TA 1535
Metabolic activation:
with and without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid
Untreated negative controls validity:
valid
Positive controls validity:
valid
Species / strain:
S. typhimurium, other: TA 97, TA 98, TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid
Untreated negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: strain/cell type: TA 1535
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
positive

In view of theses data, ACC is detectably mutagenic in bacteria.