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EC number: 204-472-0 | CAS number: 121-46-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February-April 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 8,9,10-trinorborna-2,5-diene
- EC Number:
- 204-472-0
- EC Name:
- 8,9,10-trinorborna-2,5-diene
- Cas Number:
- 121-46-0
- Molecular formula:
- C7H8
- IUPAC Name:
- bicyclo[2.2.1]hepta-2,5-diene
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and batch number of test material: Air Liquide Electronics Materials. Batch 1590035136 / 1590035137
- Purity: 100% w/w
- Manufacturing date: August 25, 2017
- Deadline to use : August 16, 2018
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature (20±5°C)
- Stability and homogeneity of the test material and during storage: stable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- In order to verify the initial concentrations and maintenance of the exposure concentrations of the test item during the ecotoxicological testing, chemical analysis in daphnids test solutions WAF) were performed according to the Analytical protocol FCBA Chimie No.386-e version 2: Method for the chemical analysis of BCHD from daphnids ecotoxicology solutions by HPLC/DAD (see study 17/1094F/c) for the Control (i.e. filtered daphnids test medium), and at the lowest, the highest concentrations and a concentration around the expected EC50 concentration.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Clone number 5 from at least the third generation obtained with a non-cyclic parthenogenesis reproduction
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 - 260 mg/L of CaCO3
- Test temperature:
- 19.5-20.0 °C
- pH:
- 7.7 - 8.1
- Dissolved oxygen:
- 8.4 - 9.3 mg/L
- Nominal and measured concentrations:
- This study was conducted on six concentrations level: 10.0 - 14.0 - 19.6 - 27.4 - 38.4 and 54.0 mg/L.
The measured concentrations of the "BCHD" did not vary more than ± 20% of nominal or initial measured concentration during the test (variations between -9.3% and +0.6%). - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 30.25 mg/L
- 95% CI:
- >= 27.92 - <= 33.15
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 15.75 mg/L
- 95% CI:
- >= 14.47 - <= 17.14
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- The last toxic response obtained on the reference item K2Cr2O7 indicates an EC50-24h of 1.59 mg/L (1.45 — 1.74), indicating that the daphnids sensitivity is correct (within the accepted interval of 0.6—2.1 mg/L fixed by the International Standard ISO 6341 December 2012 as mentioned in the OECD 202 Guideline) and in accordance with historical data obtained by the laboratory.
Any other information on results incl. tables
Nominal test item loading rate in mg/L | Number of daphnids exposed at the start | Immobilised daphnids (%) | |
at 24h | at 48h | ||
WAF Control | 20 | 0 (0%) | 0 (0%) |
WAF 10.0 | 20 | 0 (0%) | 0 (0%) |
WAF 14.0 | 20 | 0 (0%) | 5 (25%) |
WAF 19.6 | 20 | 1 (5%) | 18 (90%) |
WAF 27.4 | 20 | 2 (10%) | 20 (100%) |
WAF 38.4 | 20 | 20 (100%) | 20 (100%) |
WAF 54.0 | 20 | 20 (100%) | 20 (100%) |
In order to check the stability of the WAFs in the test solutions, concentration of BCHD in the control and the WAFs was checked by HPLC/DAD at start (t=0h), at t=24h (new and old solutions) and at the end of the test (t=48h).
Overview of the results is given in the table below:
Nominal test item loading rate | Renewal | Results of the determination of BCHD analysis (mg/L) | ||
At initial time (fresh solultions) | After 24 hours of exposure (Old solutions) | Variation (%) | ||
WAF 10.0 mg/L | T0h to T24h | 2.62 | 2.46 | • 6.1 |
WAF 10.0 mg/L | T24h to T48h | 6.53 | 5.92 | - 9.3 |
WAF 27.4 mg/L | T0h to T24h | 7.56 | 7.32 | - 3.2 |
WAF 27.4 mg/L | T24h to T48h | 15.9 | 16.0 | + 0.6 |
WAF 54 mg/L | T0h to T24h | 22.4 | 22.0 | - 1.8 |
WAF 54 mg/L | T24h to T48h | NA | NA | NA |
NA: Not Applicable (no rewal for the highest concentration at T24h because all the daphnids were dead)
According to the results of the BCHD analysis and since the study was carried out using WAFs, the results were based on the nominal test item loading rates.
Validity criteria conformity:
| Values for OECD 202 | Validity criteria for the test |
Immobilised daphnids in control | < 10% | Ok (0%) |
Dissolved Oxygen concentration | 3 mg/L | Ok (8.4 mg/L) |
All the validity criteria were successful.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- All the validity criteria were successful.
The results obtained for the test item "BCHD" on the toxicological endpoint "immobilisation" give an EL50-24h = 30.25 mg/L [27.92-33.15] and an EL50-48h = 15.75 mg/L [14.47 - 17.14] based on nominal test item loading rate. - Executive summary:
The aim of this study was to determine the acute toxicity of the test item "BCHD" on the daphnids Daphnia magna, according to the OECD guideline 202. The daphnids were exposed to Water Accommodated Fractions (WAFs) of the test item, following the 'Guidance document on aquatic toxicity testing of difficult substances and mixtures" (OECD No. 23).
Following the results of a preliminary study, a final test at six nominal loading rate was performed, in order to evaluate the loading rate which immobilise X% of the daphnids exposed to the test item after 24 and 48 hours, reported as ELx-24h and ELx-48h, relative to tested concentrations.
The immobility of the daphnids was determined in a closed semi-static 48-hours test by visual observation after 24 and 48 hours.
In order to check the stability of the WAFs in the test solutions, concentration of BCHD in the control and the WAFs was checked by HPLC/DAD at start (t=0h), at t=24h (new and old solutions) and at the end of the test (t=48h).
According to the results of the BCHD analysis and since the study was carried out using WAFs, the results were based on the nominal test item loading rates.
Validity criteria conformity: All the validity criteria were successful.
The obtained for the test item "BCHD" on the toxicological endpoint "immobilisation" give an EL50-24h = 30.25 mg/L [27.92 - 33.15] and an EL50-48h = 15.75 mg/L [14.47 - 17.14] based on nominal test item loading rate.
These values are based on nominal test item "BCHD" loading rate.
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