1,3-dichloropropane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Remarks:

GESTIS selected data

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

1980

Reference 2

Reference Type:

review article or handbook

Title:

GESTIS Substance Database 1,3-Dichloropropane

Author:

IFA Institue for Occupational Safety and Health of the German Social Accident Insurance

Year:

2018

Bibliographic source:

http://gestis-en.itrust.de/pdf/create/Data_sheet.php

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians. U.S. EPA 1975

Deviations:

yes

Remarks:

described in detail in study report

Principles of method if other than guideline:

The Guideline is published by U.S. EPA in Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians. Ecological Research Series (EPA-660/3-75-009), 61 pp. (1975)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,3-dichloropropane

EC Number:

205-531-3

EC Name:

1,3-dichloropropane

Cas Number:

142-28-9

Molecular formula:

C3H6Cl2

IUPAC Name:

1,3-dichloropropane

Test material form:

liquid

Specific details on test material used for the study:

Tested chemicals were purchased from commercial chemical suppliers and had a minimum purity of 80%.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Remarks:

Test water was identical with culture water (see Details on test organisms)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

24 h old. 3 replicates with each 5 animals
Culture water was reconstituted according to U.S. EPA (Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians. Ecological Research Series (EPA-660/3-75-009), 61 pp., 1975), to a total hardness of 173 +/- 13 mg/L (CaCO3) and a pH of 8.0 +/- 0.2

Study design

Test type:

static

Water media type:

freshwater

Remarks:

incubation water identical with culture water

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

24 h also tested

Test conditions

Hardness:

173 +/- 13 mg/L as CaCO 3

Test temperature:

22 +/- 1 °C

pH:

8.0 +/- 0.2; the range of pH values measured was 7.4-9.4.

Dissolved oxygen:

greater than 60% of saturation. Dissolved oxygen concentration and temperature were measured with a combined oxygen-temperature probe (YSI Model 54BP dissolved oxygen meter). Dissolved oxygen concentrations ranged from 6.5 to 9.1 mg/L during the 48 h exposure period. Within any one test, the greatest range observed was 6.6-8.1 mg/L.

Salinity:

fresh water, reconstituted according to U.S. EPA: Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians. Ecological Research Series (EPA-660/3-75-009), 61 pp. (1975), identical with Culture water

Conductivity:

not measured

Nominal and measured concentrations:

nominal

Reference substance (positive control):

not specified

Remarks:

testing of several substances of diverse chemical groups

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

>= 200 - <= 390 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: LC50 = 280 mg/L

Effect concentrations 2

Key result

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

>= 360 - <= 710 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: LC50 = 490 mg/L

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 68 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

LC50 Daphnia magna (48 hours): 280 mg/L

Executive summary:

Short term toxicity to aquatic invertebrates was measured according to U.S. EPA in Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians. Ecological Research Series (EPA-660/3-75-009), 61 pp. (1975), which is in principle similar to current guidelines. The LC50 of 1,3 -Dichloropropane on Daphnia magna (48 hours) is 280 mg/L.