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Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2006-01-23 to 2006-02-27
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
soil
Specific details on test material used for the study:
Identity: TIC2782 (T002907)
Batch: 00467090 (= charge 05C0836)
Purity: 99.9 %
Appearance: Solid/white
Molecular weight: 158.15 g/mol
Expiration date: May 04, 2006
Storage: At room temperature at about 20°C
Radiolabelling:
no
Test temperature:
25°C
Details on study design: HPLC method:
EQUIPMENT
- Apparatus: Varian Pump Prostar 230, Varian Autosampler 9100, Varian UV/VIS Detector ProStar 310, Jones Column Oven 7990.
- Type: HPLC with UV-VIS detector, reversed phase column and aqueous mobile phase.
- Type, material and dimension of analytical (guard) column: LiChrospher 100 CN, particle size 5 µm, 250 mm - 4 mm.
- Pre-column: CN
- Detection: 210 nm for test substance, 210 nm for reference items and sodium nitrate

MOBILE PHASES
- Type: water/acetonitrile (45:55; v/v)
- pH: 6.24
- Solutes for dissolving test and reference substances: acetonitrile

DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column (sodium nitrate)

REFERENCE SUBSTANCES
- Identity: combined reference solution containing acetanilide, isoproturon, linuron, pyrazophos, diclofop-methyl and 2,4-DDT

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 10 mL of a 298.05 µg/mL solution in solvent mixture water/acetonitrile (45:55; v/v).
- Quantity of reference substances: 10 mL of the combined reference solution (solvent mixture water/acetonitrile (45:55; v/v))
- Intervals of calibration: no data

REPETITIONS
- Number of determinations: 3 times (test item solution), 6 times (combined reference solution), 2 times (unretarded item solution)

EVALUATION
- Calculation of capacity factors k': from retention time of reference items, the test item, and the unretarded item
- Determination of the log Koc value: a correlation of log k' versus log Koc of the reference items is plotted using linear regression - the log k' value of the test item is then used to calculate its log Koc value
Type:
log Koc
Value:
0.5 dimensionless
Temp.:
25 °C
Remarks on result:
other: mean of 3 analyses
Details on results (HPLC method):
- Retention times of reference substances used for calibration:
* Acetanilide: 2.832 - 2.842 min.
* Isoproturon: 3.281 - 3.293 min.
* Linuron: 3.643 - 3.656 min.
* Pyrazophos: 4.131 - 4.145 min.
* Diclofop-methyl: 4.739-4.755 min.
* 2,4-DDT: 5.853 - 5.871 min.
- Details of fitted regression line (log k' vs. log Koc): slope = 0.094, intercept = 0.153, coefficient of regression = 0.994
- Average retention data for test substance: 2.64 min
- Koc test substance = 2.98
Validity criteria fulfilled:
yes
Conclusions:
The mean log Koc of T002907 was determined to be 0.5 using the HPLC estimation method according to OECD guideline 121. These results indicate that T002907 will not be adsorbed by organic carbon in soil. T002907 is very mobile. The results of the test can be considered reliable without restriction.

Description of key information

This endpoint is covered with a GLP-study (Weissenfeld, 2006) performed according to standard test guideline OECD 121. The mean log Koc of T002907 was determined to be 0.5 using the HPLC estimation method. These results indicate that T002907 will not be adsorbed by organic carbon in soil. T002907 is very mobile.

Key value for chemical safety assessment

Koc at 20 °C:
3

Additional information

[LogKoc: 0.5]