(2-hydroxypropyl)trimethylammonium formate

Hydrolysis

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 111 (Hydrolysis as a Function of pH)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Identification: Hydroxypropyl, 2-, trimethylammonium formate
Other name: Solvent-free Dabco TMR-2
Physical state/appearance: colorless to very light blue liquid
Batch: chernil.20160628.B
CAS Number: 62314-25-4
EU Number: 263-503-6
Purity: 95.25% active in water
Expiry date: 28 June 2017
Storage conditions: Room temperature in the dark, over silica gel

Radiolabelling:

no

Analytical monitoring:

yes

Details on sampling:

The sample solutions were taken from the waterbath at various times and the pH of each solution recorded.
The concentration of the sample solution was determined by high performance liquid chromatography and mass spectroscopy (HPLC-MS)

Buffers:

The test system consisted of sterile buffer solutions at pH’s 4, 7 and 9.

Details on test conditions:

Sample solutions were prepared in stoppered glass flasks at a nominal concentration of 0.500 g/L in the three buffer solutions.
The test solutions were split into individual vessels for each data point.
The solutions were shielded from light whilst maintained at the test temperature.
Sample solutions at pH 4, 7 and 9 were maintained at 50.0 ± 0.5 °C for a period of 120 hours.

Duration:

120 h

pH:

4

Temp.:

50 °C

Initial conc. measured:

>= 0.515 - <= 0.519 g/L

Preliminary study:

The preliminary study found the substance to be hydrolytically stable.

Transformation products:

not measured

Key result

pH:

7

Temp.:

25 °C

DT50:

> 1 yr

pH:

4

Temp.:

25 °C

DT50:

> 1 yr

pH:

9

Temp.:

25 °C

DT50:

> 1 yr

Validity criteria fulfilled:

yes

Conclusions:

The estimated half-life at 25 °C, monitoring the hydroxypropyl, 2-, trimethylammonium content of the test item was >1 year at pH 4, 7 and 9

Description of key information

Assessment of hydrolytic stability was carried out using a procedure designed to be compatible with Method C.7 AbioticDegradation, Hydrolysis as a Function of pH of Commission Regulation (EC) No 440/2008of 30 May 2008 and Method 111 of the OECD Guidelines for Testing of Chemicals, 13 April2004. The results, monitoring the hydroxypropyl, 2-, trimethylammonium content of the testitem, are as follows:

Estimated half-life at 25 °C
pH 4 >1 year
pH 7 >1 year
pH 9 >1 year

Key value for chemical safety assessment

Half-life for hydrolysis:

1 yr

at the temperature of:

25 °C