(benzothiazol-2-ylthio)acetic acid

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Remarks:

in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1985-05-07 to 1985-05-10

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The translation of the study report is available only. All scientific necessary information for evaluation is available. The study was performed according to OECD guideline 404.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

(benzothiazol-2-ylthio)acetic acid

EC Number:

228-565-0

EC Name:

(benzothiazol-2-ylthio)acetic acid

Cas Number:

6295-57-4

Molecular formula:

C9H7NO2S2

IUPAC Name:

2-(1,3-benzothiazol-2-ylsulfanyl)acetic acid

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): Praeparat EK, Acetic acid (2-benzothiazolylthione)
- Physical state: white powder
- Purity: 100%
- Purity test date: n.a.
- Impurities (identity and concentrations): n.a.
- Lot/batch No.: n.a.

Test animals

Species:

rabbit

Strain:

New Zealand White

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

0.5 g/animal

Duration of treatment / exposure:

4 hours

Observation period:

72 hours

Number of animals:

6 (3 males and 3 females)

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

overall irritation score

Basis:

mean

Remarks:

of all 6 animals

Time point:

24/48/72 h

Score:

0

Max. score:

0

Results 2

Irritation parameter:

erythema score

Basis:

mean

Remarks:

all 6 animals

Time point:

24/48/72 h

Score:

0

Max. score:

0

Results 3

Irritation parameter:

edema score

Basis:

mean

Remarks:

all 6 animals

Time point:

24/48/72 h

Score:

0

Max. score:

0

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Under the conditions of this test and the results obtained, it is concluded, that the test substance is not irritant to the skin.

Executive summary:

In order to evaluate the irritant/corrosive potential of the test substance to the skin an in vivo test in rabbits was performed in 1985 which was in accordance to guideline OECD 404. 24 hours prior to treatment the animals back was shaved to receive a skin area of about 10 x10 square centimeters for treatment. Only animals with intact skin were chosen for the test. The 6 animals were each treated with 0.5 g test substance per animal on the right flank, whereas the left side serves as control. The treated area was covered by an occlusive bandage and fixed with a non-irritant tape for 4 hours. After these 4 hours, the bandage was removed and the skin washed with lukewarm water to remove remaining test substance. Mortality and clinical signs of toxicity were observed once daily. The skin was assessed for edema, eschar formation or erythema according to Draize, 60 minutes, 24, 48 and 72 hours after removing of bandage. No mortality occurred. No clinical signs of toxicity were observed. The overall mean scoring value is 0 for erythema and edema formation.

Under the conditions of this test and the results obtained, it is concluded, that the test substance is not irritant to the skin.