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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
5-3-03 to 7-3-03
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot No.of test material: Sponsor and #005Y030

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Dark room at room temperature
- Stability under test conditions: Stable
- Solubility and stability of the test substance in the solvent/vehicle: 40 g/L
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: None

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Final dilution of a dissolved solid, stock liquid or gel: 500 mg/L, 200 mg/L, 80 mg/L, 32 mg/L, 13 mg/L dilutions of the solid in the prepared freshwater were prepared.

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 500 mg/L, 200 mg/L, 80 mg/L, 32 mg/L, 13 mg/L dilutions of the solid in the prepared freshwater
- Sampling method: Physical observation of health of the daphnids

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: A freshwater solution without the substance being tested was prepared as a negative control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): H2O
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): 500 mg/L, 200 mg/L, 80 mg/L, 32 mg/L, 13 mg/L dilutions of the tested solid in the prepared freshwater
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): None

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Source: Toxikon Corporation, Jupiter, FL
- Age of parental stock: gravid females
- Feeding during test: No
- Amount: 100

ACCLIMATION
- Acclimation period: 24 hours
- Acclimation conditions: Isolatred in food-free dilution water
- Type and amount of food: No food
- Health during acclimation (any mortality observed): No mortalities and were in good health

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Water fleas will be neonates (i.e., <24 hours old) obtained by isolating gravid females from the culture water in food-free dilution water <24 hours prior to beginning the test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
2 d
Remarks on exposure duration:
None
Post exposure observation period:
Immediately after the end of the experiment.

Test conditions

Hardness:
70 mg/L as CaCO3 at test intiation
Test temperature:
20.1 to 20.6 degrees celsius during the duratino of the 48 hour exposure.
pH:
5.7 to 7.8 during the duration of the 48 hour exposure
Dissolved oxygen:
Initial dissolved oxygen concentrations in the control and test solutions were 8.8 to 8.9 mg/L and 8.4 to 8.7 mg/L at test termination.
Salinity:
26 mg/L as CaCO3
Conductivity:
399 microS at test intiation
Nominal and measured concentrations:
Nominal Concentrations: 500 mg/L, 200 mg/L, 80 mg/L, 32 mg/L, 13 mg/L dilutions of the solid in the prepared freshwater
Details on test conditions:
TEST SYSTEM
- Test vessel: vials
- Type: closed
- Material, size, headspace, fill volume: Glass, 50 mL, none, 50 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Water origninated in Town of Jupiter
- Pesticides: <1.040 microg/L
- Chlorine: 87 mg/L
- Alkalinity: 70 mg/L as CaCO3
- Ca ratio(mg/L): 19 mg/L
- Conductivity: 399 microSiemens
- Salinity: 26 mg/L as CaCO3
- Intervals of water quality measurement: Beginning and end of test

OTHER TEST CONDITIONS
- Photoperiod: 16 hours of light / 8 hours of dark
- Light intensity: 13.2 to 13.9 microMls per square meter per second


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Dissolved oxygen content, alkalinity, hardness, and conductivity. These were measured at the beinning and end of the test. pH and temperature were measured continuously for the duration of the test.

VEHICLE CONTROL PERFORMED: Yes

RANGE-FINDING STUDY
- Test concentrations: 100 mg/L and 1000 mg/L
- Results used to determine the conditions for the definitive study: Yes
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
1 d
Dose descriptor:
EC50
Effect conc.:
ca. 350 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
2 d
Dose descriptor:
EC50
Effect conc.:
ca. 170 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
2 d
Dose descriptor:
NOEC
Effect conc.:
ca. 80 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: None
- Mortality of control: None
Reported statistics and error estimates:
95% confidence was used to determine the 1 and 2 day EC50 values. The 95% confidence range for the 1 day EC50 value is 290 to 420 mg/L. The 95% confidence range for the 2 day EC50 value is 130 to 210 mg/L. This and the 2 day NOEC value were calalculated with the computer program ToxCalc.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
This test effectively estabilshes EC50 24 hour and 48 hour and NOEC guidelines for short term toxicity to aquatic invertebrates based on the OECD 202 guideline.