Bis(4-hydroxy-N-methylanilinium) sulphate

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from secondary literature.

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

Daphnia magna Bioassay was carried out under static condition to study the effects of Bis(4-hydroxy-N-methylanilinium) sulphate on aquatic environment.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: p-Methylaminophenol sulfate- IUPAC name: Bis(4-hydroxy-N-methylanilinium) sulphate- Molecular formula: C14H20N2O6S- Molecular weight: 344.386 g/mole- Smiles:CNc1ccc(cc1)O.CNc1ccc(cc1)O.OS(=O)(=O)O- Inchl: 1S/2C7H9NO.H2O4S/c2*1-8-6-2-4-7(9)5-3-6;1-5(2,3)4/h2*2-5,8-9H,1H3;(H2,1,2,3,4)- Substance type: Organic- Physical state: Solid crystalline (off white - white)

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

- Stock solution: At the start of each bioassay, appropriate dilutions of each test chemical were prepared and dispensed into 150-milliliter Griffin beakers. The pH of each test solution was adjusted to between pH 7.07.5.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM- Common name: water flea- Source: Carolina Biological Supply company, Burlington North carolina.- Method of breeding: A more satisfactory culture medium was obtained by blending approximately 2 grams of alfalfa pellets with 3-4 dried brine shrimp per liter of water. This medium was replaced every 2-3 days and was successful in promoting reproduction of the stock culture.- Food type: algae

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

54.5 (37.0-75.0) F

pH:

7.0-7.5

Details on test conditions:

- Test vessel: one-gallon, wide-mouth, - Material, size, headspace, fill volume: glass jars

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.019 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: : toxic

Bioassay data summary of Daphnia Magna

			Cumulative mortalities				Percent mortality at
Test chemical	Conc.(mg/l)	Starting population	24 hr	48 hr	72 hr	96 hr	96 hrs	96hr LC50 or range (mg/l)
4-methyl Amino Phenol Sulfate (VIIID8)	0.3	11	2	4	9	9	81.8	0.019
	0.09	11	1	3	7	8	72.7
	0.03	10	2	3	4	7	70.0
	0.009	10	1	1	2	4	40.0
	0.0	53	1	2	2	4	7.5

Conclusions:

The lethal concentration LC50 to 50% of daphnia magna is 0.019 mg/L at 96 hrs. Mortality was measured during the test. It can be concluded from the value that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic environment.

Executive summary:

Daphnia magna Bioassay was carried out under static condition to study the effects of Bis(4-hydroxy-N-methylanilinium) sulphate on aquatic environment. At the start of each bioassay, appropriate dilutions of each test chemical were prepared and dispensed into 150 milliliter Griffin beakers. The pH of each test solution was adjusted to between pH 7.0-7.5. A final volume of 100-110 milliliters was used throughout the testing program. Controls, which contained only Daphnia in dilution water, were included in every bioassay. Adult mortalities were recorded on a daily basis. Births and newborn mortalities observed during the experiment were noted but not enumerated. The lethal concentration LC50 to 50% of daphnia magna is 0.019 mg/L at 96 hrs. It can be concluded from the value that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic environment and can be considered as “aquatic Chronic 1” as per classification for the aquatic environment.

Description of key information

Daphnia magna Bioassay was carried out under static condition to study the effects of Bis(4-hydroxy-N-methylanilinium) sulphate on aquatic environment. At the start of each bioassay, appropriate dilutions of each test chemical were prepared and dispensed into 150 milliliter Griffin beakers. The pH of each test solution was adjusted to between pH 7.0-7.5. A final volume of 100-110 milliliters was used throughout the testing program. Controls, which contained only Daphnia in dilution water, were included in every bioassay. Adult mortalities were recorded on a daily basis. Births and newborn mortalities observed during the experiment were noted but not enumerated. The lethal concentration LC50 to 50% of daphnia magna is 0.019 mg/L at 96 hrs. It can be concluded from the value that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic environment and can be considered as “aquatic Chronic 1” as per classification for the aquatic environment.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.019 mg/L

Additional information

Daphnia magna Bioassay was carried out under static condition to study the effects of Bis(4-hydroxy-N-methylanilinium) sulphate on aquatic environment. At the start of each bioassay, appropriate dilutions of each test chemical were prepared and dispensed into 150 milliliter Griffin beakers. The pH of each test solution was adjusted to between pH 7.0-7.5. A final volume of 100-110 milliliters was used throughout the testing program. Controls, which contained only Daphnia in dilution water, were included in every bioassay. Adult mortalities were recorded on a daily basis. Births and newborn mortalities observed during the experiment were noted but not enumerated. The lethal concentration LC50 to 50% of daphnia magna is 0.019 mg/L at 96 hrs. It can be concluded from the value that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic environment and can be considered as “aquatic Chronic 1” as per classification for the aquatic environment.