α,α,α-trichlorotoluene

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The authors tested the biodegradability of benzotrichloride according to the OECD guideline 301E. GLP standards are not specified and no data are available for validity criteria fulfillment. However, the study is sufficiently described. Thus the study should be considered a Klimisch 2c study as it is comparable to a guideline study with acceptable restrictions.

Data source

Reference

Reference Type:

publication

Title:

Versuche zur biologischen Abbaubarkeit chlororganischer Verbindungen

Author:

Steinhäuser K.G., Amann W. and Polenz A.

Year:

1986

Bibliographic source:

Vom Wasser, 67, 147-154.

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

Deviations:

yes

Remarks:

one replicate only

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

α,α,α-trichlorotoluene

EC Number:

202-634-5

EC Name:

α,α,α-trichlorotoluene

Cas Number:

98-07-7

Molecular formula:

C7H5Cl3

IUPAC Name:

α,α,α-trichlorotoluene

Constituent 2

Reference substance name:

trichloromethylbenzene

IUPAC Name:

trichloromethylbenzene

Details on test material:

- Name of test material (as cited in study report): Benzotrichloride
No more data available

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): predominantly domestic sewage
No more data available

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

5.4 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

22 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST SYSTEM
- Number of culture flasks/concentration: 2 concentrations; 5.4 and 22 mg/L
No more data available


SAMPLING
- Sampling frequency: Weekly with an extra time point after 3 days
No more data available


CONTROL AND BLANK SYSTEM
- Toxicity control: yes
No more data available


STATISTICAL METHODS:
No data available

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

Preliminary study:

No data available

Test performance:

No data available

% Degradation

% Degradation 1

Parameter:

% degradation (DOC removal)

Value:

95

Sampling time:

3 d

Remarks on result:

other: Test substance concentration 5.4 mg/L

% Degradation 2

Parameter:

% degradation (DOC removal)

Value:

96

Sampling time:

7 d

Remarks on result:

other: Test substance concentration 5.4 mg/L

% Degradation 3

Parameter:

% degradation (DOC removal)

Value:

95

Sampling time:

3 d

Remarks on result:

other: Test substance concentration 22 mg/L

% Degradation 4

Parameter:

% degradation (DOC removal)

Value:

97

Sampling time:

7 d

Remarks on result:

other: Test substance concentration 22 mg/L

BOD5 / COD results

Results with reference substance:

No data

Any other information on results incl. tables

- Chloride formation by degradation of the test substance was also measured (see table below)

- A control for abiotic degradation (precipitation, adsorption and volatilisation) of the test substance was performed (test substance without inoculum) (see table below)

Table:

	Initial concentration testsubstance (mg/L)	DOC removal in % (days)					Chloride formation by degradation in % (days)
		3	7	14	21	28	0	3	7	14	21	28
	5.4	95	96				105	111	108
	22	95	97				103	100	112
Control for abiotic degradation	5.5	1	11				102	110	107

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable

Conclusions:

In the test conditions, benzotrichloride was assessed to be readily biodegradable in an OECD guideline 301E test after 7 days of incubation.

Executive summary:

The authors tested the biodegradability of the benzotrichloride (CAS n° 98 -07 -7) according to the OECD guideline 301E. They also monitored the formation of the main degradation product, inorganic chloride. For a concentration of 5.4 and 22 mg/L, 96% and 97% of the DOC respectively was removed after 7 days. They confirmed abiotic degradation did not occur by testing a control without innoculum. Thus, at this level of information, benzal chloride should be considered as readily biodegradable according to the EC regulation n° 1272/2008 and the former 67/548/EC regulation.

No data was available on the GLP status of the study. None of the validity criteria was sufficiently documented to check their fulfillment. However, the authors followed the OECD guideline 301E and documented sufficiently their study to consider this study as reliable with restrictions and comparable to a guideline study with acceptable restrictions.