Registration Dossier
Registration Dossier
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EC number: 279-301-6 | CAS number: 79817-70-2
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: screening test, other
- Remarks:
- Inherent Biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- not applicable
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1',2'-dihydro-6'-hydroxy-3,4'-dimethyl-2'-oxo-1,3'-bipyridinium chloride
- EC Number:
- 279-301-6
- EC Name:
- 1',2'-dihydro-6'-hydroxy-3,4'-dimethyl-2'-oxo-1,3'-bipyridinium chloride
- Cas Number:
- 79817-70-2
- Molecular formula:
- C12H13N2O2.Cl
- IUPAC Name:
- 6'-hydroxy-3,4'-dimethyl-2'-oxo-1',2'-dihydro-1,3'-bipyridinium dichloride
- Test material form:
- liquid
- Details on test material:
- Active su
Constituent 1
- Specific details on test material used for the study:
- HMPPJC, dark brown liquid in original container at room temperature
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- A mixed population of activated sewage sludge microorganism
- Duration of test (contact time):
- >= 28 d
Initial test substance concentration
- Initial conc.:
- ca. 108.9 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
Reference substance
- Reference substance:
- diethylene glycol
- Remarks:
- Digol
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- ca. 24
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Interpretation of results:
- not inherently biodegradable
- Executive summary:
HMPPJC attained 24% degradation after 28 days and so, therefore, cannot be considered as inherently biodegradable udner the stric terms and conditions of the OECD Guidelines.
The standard material, Digol, attained 99%degradation after 28 days, thereby confirming the suitability of the inoculum and culture conditions.
pF values did not drop below 6.7 in any culture vessel during the course of the study and were adjusted to pH 7.4 daily. Dissolved oxygen levels remained at approximately 6.4 mg O2/l or above in all cultures at all times.
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