Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
The toxicity of chloroform as determined by single and repeated exposure of laboratory animals
Author:
Torkelson TR, Oyen F, Rowe VK
Year:
1976
Bibliographic source:
American Industrial Hygiene Association Journal 37(12), 697-705

Materials and methods

Principles of method if other than guideline:
no data
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Chloroform
EC Number:
200-663-8
EC Name:
Chloroform
Cas Number:
67-66-3
Molecular formula:
CHCl3
IUPAC Name:
chloroform
Test material form:
liquid

Test animals / tissue source

Species:
rabbit
Strain:
not specified
Details on test animals or tissues and environmental conditions:
no data

Test system

Vehicle:
unchanged (no vehicle)
Controls:
no
Amount / concentration applied:
no data, liquid chloroform as specified above
Observation period (in vivo):
at least one week after application
Number of animals or in vitro replicates:
three
Details on study design:
single instillation of liquid chloroform into the rabbit's eye; washing of one eye with water 30 seconds after instillation

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: Not given in the study
Score:
ca. 1
Reversibility:
not fully reversible within: after one week
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: Not given in the study
Score:
ca. 1
Reversibility:
other: Barely discernible after one week
Irritant / corrosive response data:
slight irritation of the conjunctiva, which was barely detectable after one week; slight but definite corneal injury as evidenced by staining with fluorescein
Other effects:
purulent exudate occurred two or more days after treatment

Any other information on results incl. tables

The irritation effects were not evaluated following common methods such as the Draize's method and scores cannot be quantified.

Applicant's summary and conclusion

Interpretation of results:
irritating
Remarks:
Migrated information
Executive summary:

Liquid chloroform was instilled into the eye of three rabbits, with and without washing of the eye after treatment. Chloroform was a slight irritant to the conjunctiva, which was reversible after one week. In addition, slight but irreversible corneal injury occurred as evidenced by staining with fluorescein. A purulent exudate occurred for two or more days after treatment. In conclusion, chloroform was found to be irritating to the rabbit's eye.