Registration Dossier

Administrative data

Endpoint:
short-term repeated dose toxicity: oral
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Adopted according to OECD SIDS (public available peer reviewed source). The original source is available and has been reviewed.
Cross-reference
Reason / purpose:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
The effect of dichlorobutene on living organisms and its relation to occupational loss of hair observed in people working in the manufacture of chloroprene rubber.
Author:
Roubal J and Pokorny F
Year:
1943
Bibliographic source:
Casopia Lekaru Ceskych 82:901-20
Reference Type:
secondary source
Title:
1,3-Dichlorobut-2-ene - CAS No: 926-57-8
Author:
OECD SIDS
Year:
2007
Bibliographic source:
SIDS Initial Assessment Report for 22th SIAM, UNEP Publications

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The dog was fed weekly 1 cc of 1,3-dichlorobut-2-en which was mixed with flour and given to the dog in a capsule so she would swallow it
whole. The dog was fed 2.5 cc 1,3-dichlrobut-2-ene in the first 20 days.
GLP compliance:
no
Remarks:
GLP was not mandatory at the time of the study

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent

Test animals

Species:
dog
Strain:
other: Mixed black-white fox-terrier
Sex:
female

Administration / exposure

Route of administration:
oral: capsule
Details on oral exposure:
DIET PREPARATION
- Rate of preparation of diet (frequency): 1 cc of 1,3-DCB which was mixed with flour and given to the dog in a capsule
Duration of treatment / exposure:
1 month
Frequency of treatment:
once a week
Doses / concentrations
Remarks:
Doses / Concentrations:
1 cc (weekly), 2.5 cc (during first 20 days)
Basis:

No. of animals per sex per dose:
1
Control animals:
no

Results and discussion

Results of examinations

Details on results:
One hour and half after feeding, a light glassy slime returned, very viscous with an odour of 1,3-dichlorobut-2-ene. During the first week a kilo of weight was lost and at the end of the same week the hair began to fall in considerable amounts. The dog was fed 2.5 cc of 1,3-dichlorobut-2-ene in the first 20 days. The maximum loss of hair was noticed than. The gastric juices after a month feeding had a high free acid amount and a total of acidity of 106.4 which indicates an inflammation of the mucous membrane of the stomach. The tongue at the time was very dry. After a month the test was terminated, the fur began to grow fast, in good quantity and it was of glossy appearance. After 2 months from the beginning of the test the dog weighed 10.5 K.

Target system / organ toxicity

Critical effects observed:
not specified

Applicant's summary and conclusion