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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
GLP compliance:
yes
Test type:
standard acute method

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: colorless liquid
Details on test material:
purity: 85.1%

Test animals

Species:
rat
Strain:
other: Hoe: WISKf (SPF71)
Sex:
male/female

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
nose only
Vehicle:
air
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
5 m+ 5 f rats: 14.8 mg m-dichlorobenze/l air
5 m+ 5 f rats: 17.63 mg m-dichlorobenze/l air
No. of animals per sex per dose:
5 m+ 5 f rats: 14.8 mg m-dichlorobenze/l air
5 m+ 5 f rats: 17.63 mg m-dichlorobenze/l air
Control animals:
no

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 17.6 mg/L air
Based on:
test mat.
Exp. duration:
4 h

Any other information on results incl. tables

After an expostion to 17.6 mg m-dichlorobenzene no of the animals died. The test atmosphere consited maily as vapor.

Based on this result, the LC50(4h) was > 17.6 mg dichlorobenzene/l.

Of the animals exposed to 14.8 mg m-dichlorobenene 2 male and 3 female rats died. The test atmosphere had a higher content of aerosol compared with the test group which received 17.6 mg m-dichlorobenzene/l air.

The animals exposed to 17.6 mg m-dichlorobenzene/l air showed unregular breathing, uncoordinated gait, ataxia, prone position, rough fur, drowsiness, tremor, constricted palpebral fissures, reduced startled reflexes, increased salivation and foamy nose effluent.

Animals which received furthermore reduced breathing rate, staggared gait, reducted activity, narcosis, coma, no startled reflexes, bloody nose and eye lids, closed palpebral fissures, reduced body temperature, convulsions, cyanosis bad nutrition state and sneezing.

The section of animals which died intercurrent had darkred spots on the lungs, darkened liver and blood engorgement in the lungs. The animals which were killed after the post-observation period revealed no findings.

Applicant's summary and conclusion

Interpretation of results:
other:
Remarks:
LC50(4h) > 17.6 mg m-dichlorobenzene (vapour)/l air.
Executive summary:

Five male and five female Wistar rats were exposed nose-only for 4 hours to concentrations of 14.8 or 17.63 mg dichlorobenzene/liter air. Animals were observed for mortality, body weight and clinical signs during a post-observation period of 14 days. A necropsy was performed on the animals at the end of the post-observation period.

The LC50(4h) was > 17.6 mg m-dichlorobenzene (vapour)/l air.