Registration Dossier

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019
Report Date:
2019

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)
GLP compliance:
no
Type of method:
HPLC method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder

Study design

Analytical method:
high-performance liquid chromatography

Results and discussion

Partition coefficient
Type:
log Pow
Partition coefficient:
2
Temp.:
20 °C
pH:
5.92
Details on results:
The retention times of the test and reference items were determined in two replicates injecting twice both of them. The test item was dissolved in methanol:deionized water (55:45) and diluted in methanol:deionized water (55:45) to obtain a final concentration of 100 mg/l. Dead-time measured by Thiourea was 0.932 minutes. In order to correlate the calculated capacity factor k of test item with its logPow, a calibration graph using six reference substances were used. The value of partition coefficient of 15α-Hydroxy-androstendione (CAS No. 566-08-5) was determined by Microsoft Office Excel 2013: logPow= 2.0

Applicant's summary and conclusion

Conclusions:
The calculated mean logPow of 15α-OH-AD: 2.0