β-methylaziridine-1-propiononitrile

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well-documented, scientifically acceptable study report

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Principles of method if other than guideline:

according to H.F. Smyth et al., Am. Ind. Hyg. Ass. J. 23, 95 - 107 (1962)

GLP compliance:

no

Test type:

other: inhalation hazard test

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

β-methylaziridine-1-propiononitrile

EC Number:

223-798-4

EC Name:

β-methylaziridine-1-propiononitrile

Cas Number:

4078-19-7

Molecular formula:

C6H10N2

IUPAC Name:

3-(aziridin-1-yl)butanenitrile

Test animals

Species:

rat

Sex:

male/female

Details on test animals or test system and environmental conditions:

Young adult laboratory rats were purchased from a breeder.

Administration / exposure

Route of administration:

inhalation: vapour

Details on inhalation exposure:

200 L air/ h were run through a 5 cm thick layer of the test substance. The atmosphere temperature was 20 °C. The atmosphere was saturated with vapour .

Remarks on duration:

10 min, 30 min, 1 h, 3 h, 7 h

Concentrations:

10 min: 4.5 mg/L = 998 ppm
30 min: 2.1 mg/L = 445 ppm
1 h: 2.15 mg/L = 477 ppm
3 h: 1.08 mg/L = 259 ppm
7h: 1 mg/L = 222 ppm

No. of animals per sex per dose:

10 min, 30 min and 60 min exposures: 12 (each 3 male and female)
3 h and 7 h exposures: 6 (each 3 male and female)

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC100

Effect level:

ca. 0.81 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Effect levels 2

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 0.54 mg/L air

Exp. duration:

4 h

Remarks on result:

other: calculated from 1h exposure

Mortality:

10 min exposure: 0/12
30 min and 60 min exposure: 1/12
3 h and 7 h exposure: 6/6

Clinical signs:

other: Attempts to escape, accelerated respiration, intermittently breathing, reddish nasal secretion, snout wiping, crouched position, ruffled fur, apathy, unsteady - restlessly - staggering - trembling gait

Gross pathology:

Animals that died: heart: acute dilatation; acute congestive hyperemia; lung: slight enhanced blood content; liver: partly slight brightened; kidneys: whitish top of the papilla.
Sacrificed animals: partly white-yellowish papilla.

Applicant's summary and conclusion

Interpretation of results:

toxic

Remarks:

Migrated information

Conclusions:

The test substance was shown to be toxic via inhalation to rats. LC50 is calculated to be 0,5375

Executive summary:

Rats were exposed to the vapour of the test substance. After 10 min of exposure no death was observed. After 30 min and 1 h 1/12 animals died while after 3 and 7 h of exposure 6/6 animals were dead. The experimental set up did not allow to calculate a LD50 but showed that prolonged inhalation of a saturated atmosphere (vapour) led time-dependently to increased mortality.