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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Study period:
From May 22, 1989 to May 26, 1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to accepted guidelines in compliance with GLP.
Justification for data waiving:
other:
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
other: ISO-guideline 7346/2
Deviations:
no
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
Principles of method if other than guideline:
Not applicable
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Castor oil, hydrogenated
- Analytical purity: 100%

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct weighing of the test substance into the test vessels, fill up with standard dilution water, then stirring 5 min by ultraturax.
- Eluate: Water

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish
- Feeding during test: No


Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
250 mg CaCO3/L
Test temperature:
22.5 - 23.5°C
pH:
7.6 - 8
Dissolved oxygen:
68 - 97%
Nominal and measured concentrations:
0, 1,000, 3,000 and 10,000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Fish basins
- Material, size, headspace, fill volume: 10 L
- Aeration: Oxygen saturated
- Renewal rate of test solution (frequency/flow rate): Daily
- Number of fish per concentration: 10

OTHER TEST CONDITIONS
- Photoperiod: 16 h illumination per d

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality

TEST CONCENTRATIONS
- Test concentrations: 0, 1,000, 2,000 and 10,000 mg/L
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: None
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: None

Any other information on results incl. tables

None.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the study, the 96h LC50 of 12-hydroxystearic acid to Zebra fish (Brachydanio rerio) was determined to be > 1000 mg/L.
Executive summary:

A study was conducted to determine the acute toxicity of the test substance to Zebra fish (Brachydanio rerio), in accordance with ISO-Guideline 7346/2 which conforms to the OECD Guideline 203 and in compliance with GLP.

The substance was tested in a concentration range of 1,000 - 10,000 mg/L and the incubation period was 96 h under semi-static conditions.

Under the conditions of the study, the 96h LC50 of test substance to Zebra fish was determined to be > 1,000 mg/L.