3,5,8-Trioxa-4-phosphaundec-10-en-1-aminium, 4-hydroxy-N,N,N,10-tetramethyl-9-oxo-, inner salt, 4-oxide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

other: Letter of Access (See Section 13)

Adequacy of study:

key study

Data source

Reference

Reference Type:

other: Letter of Access (See Section 13)

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Deviations:

not specified

GLP compliance:

not specified

Remarks:

Letter of Access Only

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

417-560-0

EC Name:

-

Cas Number:

67881-98-5

Molecular formula:

C11H22NO6P

IUPAC Name:

trimethyl[2-({2-[(2-methylprop-2-enoyl)oxy]ethyl phosphonato}oxy)ethyl]azanium

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

28 d

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

3

St. dev.:

0

Sampling time:

4 d

Remarks on result:

other: St dev data unavailable

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

19

St. dev.:

0

Sampling time:

7 d

Remarks on result:

other: St dev data unavailable

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

51

St. dev.:

0

Sampling time:

14 d

Remarks on result:

other: St dev data unavailable

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

57

St. dev.:

0

Sampling time:

21 d

Remarks on result:

other: St dev data unavailable

% Degradation 5

Parameter:

% degradation (CO2 evolution)

Value:

61

St. dev.:

0

Sampling time:

28 d

Remarks on result:

other: St dev data unavailable

Details on results:

After 28 days the sample attained oxygen depletion equivalent to 61 % biodegradation relative to theoretical oxygen demand. After nitrate and nitrite analysis this was adjusted to 67 % carbonaceous biodegradation. There was no evidence of inhibitory effects to sewage bacteria, under the conditions of the test.

BOD5 / COD results

Results with reference substance:

Refer to Table 1.

Any other information on results incl. tables

Table 1. Raw Data from Closed Bottle Test

Test Item		Reference Substance
Day	% Biodegradation	Day	% Biodegradation
4 7 14 21 28	3 19 51 57 61	4 7 14 21 28	58 65 68 64 59

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable, but failing 10-day window

Conclusions:

The results of this test indicate that HEMA-PC is readily biodegradable, but fails the 10-day window.

Executive summary:

This study was conducted to determine the ready biodegradability of HEMA-PC using EU Method C.4 -E (Closed Bottle Test). After 28 days the sample attained oxygen depletion equivalent to 61 % biodegradation relative to theoretical oxygen demand. After nitrate and nitrite analysis this was adjusted to 67 % carbonaceous biodegradation. There was no evidence of inhibitory effects to sewage bacteria, under the conditions of the test.

The results of this test indicate that HEMA-PC is readily biodegradable, but fails the 10-day window.