1,1,3,3-tetramethylbutylamine

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment. Data from collection of data.

Data source

Reference

Reference Type:

publication

Title:

Acute Toxicities of Organic Chemicals to Fathead Minnows (Pimephalis promelas)

Author:

Geiger, D.L. et al.

Year:

1988

Bibliographic source:

Vol. 4, 189 - 190. Center for Lake Superior Environmental Studies, Univ. of Wisconsin, Superior, WI. (1988)

Materials and methods

Principles of method if other than guideline:

Method: according to U.S. EPA guideline, method similar to OECD guideline no. 203

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,1,3,3-tetramethylbutylamine

EC Number:

203-491-1

EC Name:

1,1,3,3-tetramethylbutylamine

Cas Number:

107-45-9

Molecular formula:

C8H19N

IUPAC Name:

2,4,4-trimethylpentan-2-amine

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Sampling points were 0, 24, 48 and 72 h.

Test solutions

Details on test solutions:

Stock solution: 130 mg/L

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: Fathead minnow
- Source: US EPA Environmental Research Laboratory-Duluth or University of Wisconsin-Superior campus
- Age at study initiation: 29 days
- Length at study initiation: 19.7 +/- 2.0 mm
- Weight at study initiation (mean and range, SD): 0.119 +/- 0.037 g
- Feeding before test: frozen adult brine shrimp (Artemia sp.)
- Fish were not fed 24 h before and during the test

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

44.3 +/- 0.50 mg/L CaCO3

Test temperature:

24.3 +/- 0.26 °C

pH:

7.7 +/- 0.03

Dissolved oxygen:

7.5 +/- 0.27

Nominal and measured concentrations:

nominal concentrations: 0, 9.94, 15.3, 23.5, 36.1 and 55.6 mg/L
measured concentrations: <1.15, 9.22, 14.9, 24.2, 37.9 and 60.1 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
Tank volume: 1.0 L
- No. of organisms per concentration: 20
- Biomass loading rate: 2.380 g/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

24.6 mg/L

Nominal / measured:

meas. (arithm. mean)

Basis for effect:

mortality (fish)

Remarks on result:

other: Conf. Lim. 22.1-27.2 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

24.6 mg/L

Nominal / measured:

meas. (arithm. mean)

Basis for effect:

behaviour

Remarks:

lost schooling behaviour, hyperactivity, overreactivity to external stimuli, increased respiration

Remarks on result:

other: Conf. Lim. 22.1-27.2 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

In this short-term fish toxicity test an LD50 value of 24.6 mg/L was obtained.