Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-050-0
CAS number: 91-21-4
In an in vitro EpiDerm™ Skin Corrosion Test (non-GLP, similar to OECD TG 431) 1,2,3,4-Tetrahydroisoquinoline was found to be moderately corrosive (viability reduced to 47% and 14% after exposure for 3 minutes and 1 hour, respectively). Skin effects indicative of corrosivity were also observed in the acute dermal toxicity test in rats (24 hours exposure, semi-occlusive, to 1000 mg/kg bw).
A non-GLP in vitro EpiDerm™ Skin Corrosion
Test (similar to OECD guideline 431) was available for assessment (BASF
AG, 2006c). The corrosive potential of 1,2,3,4-Tetrahydroisoquinoline
was predicted from the mean relative (to negative control) tissue
viabilities obtained after exposure periods of 3 minutes or 1 hour (two
tissues per exposure condition). The study included a concurrent
positive control which showed the expected marked reduction of viability
(to 20% and 9% after 3 minutes and 1 hour, respectively). A substance is
considered corrosive to skin (i) if the viability after 3 minutes
exposure is less than 50%, or (ii) if the viability
after 3 minutes exposure is ≥ 50% and that after 1 hour exposure <15%.
The test material was applied undiluted (pH undiluted: ca. 8). The mean
tissue viability after exposure to the test material was 47% (3 minutes)
and 14% (1 hour), indicating that the test material was corrosive to
skin. Though the test method does not yet allow for the differentiation
of severity of the effect, the results indicated moderate corrosivity.
Skin effects indicative of corrosivity were
also observed in a GLP-compliant acute dermal toxicity test (OECD 403)
in which five male and five female Wistar rats were treated with a
single dose of 1000 mg 1,2,3,4-Tetrahydroisoquinoline/kg body weight on
the clipped skin, covered by a semi-occlusive dressing, for 24 hours
(BASF AG, 2006b). All animals were killed in a moribund state on study
day 1. On this day, the following skin effects were observed at the
application site: very slight edema, induration, dryness, and red and
dark-brown discoloration. Histopathological examination of the skin
revealed moderate to extreme epidermal and follicular necrosis with
pyknosis, and necrotic epidermis remaining in situ. Minimal inflammatory
infiltrates were observed in 1 male animal.
Effects observed in repeated-dose inhalation
toxicity studies with 1,2,3,4-Tetrahydroisoquinoline in rats
(GLP-compliant 90-day study according to OECD TG 413 and pertaining
14-day range-finding study) indicated irritation of the respiratory
tract (BASF SE, 2012). A summary of the methods and results of the
90-day study is given in the endpoint summary under 7.5 ‘Repeated dose
toxicity’. Results indicative of respiratory irritation included
clinical symptoms (salivation, reduced fur care, escape attempts) and
histopathological changes at the base of the epiglottis (epithelial
alteration, squamous metaplasia, submucosal lymphohistiocytic
inflammation) at the mid- and/or high-concentration (24.7 and 75.1 mg/m³
, respectively). Histopathology results of the range-finding study
revealed strong irritation of the upper respiratory tract at the
high-concentration of 150 mg/m³ (level I nasal cavity: degeneration of
squamous epithelium, in some animals with ulceration; level I larynx:
epithelial alteration, mucous cell metaplasia and lympoid cell
infiltration; large bronchi: mild mucous cell hyperplasia).
Based on the in vitro skin corrosion test,
1,2,3,4-Tetrahydroisoquinoline needs to be classified as corrosive to
the skin and eyes according to Directive 67/548/EEC (C; R34 Causes
burns) and according to the EU Classification, Labelling and Packaging
of Substances and Mixtures (CLP) Regulation (EC) No. 1272/2008 (Cat. 1B,
H314: Causes severe skin burns and eye damage).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Na ovom portalu koristimo kolačiće kako bismo vam osigurali najbolje iskustvo njegova pregledavanja.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again