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EC number: 888-364-4 | CAS number: 146569-48-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- effects on growth of green algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2021-09-24 to 2021-11-18, with the definitive exposure phase from 2021-11-09 to 2021-11-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- (2016)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- (2011)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Test item Mo10V3TeNbO42
Batch number EX.14402.600
CAS No. 146569-48-4
Active ingredient
Niobium trivanadium decamolybdenum tellurium dotetracontaoxide
Purity 87.6%
Mo content 47.87 % (stoichiometric content)
Water solubility of Mo 11.498 mg/L (at pH 8.5, after 4 d shaking at 100 rpm of 100 mg/L Mo10V3TeNbO42; Affolter, 2020)
Appearance Black solid powder
Water solubility < 0.2 g/L practically insoluble
Stability under test conditions Not specified
Expiry date 2026-03-10
Recommended storage Store tightly closed, cool and dry / 20 °C - Analytical monitoring:
- yes
- Remarks:
- via ICP-OES at the start (0 hours) and at the end of the exposure (72 hours) without algae
- Details on sampling:
- Chemical Analysis of the Test Item Concentrations
The molybdenum concentration was determined via element analysis of Mo, processed at the test site. Test item samples were labelled with the test number, the concentration, the code for the sampling times, the replicate number, the date and the sampler, according to the test facility SOP and delivered to the test site for the analytical delegated phase. A full chain of custody was maintained. A phase plan with the description of the method used for the delegated phase was added to the study plan.
Sampling schedule
All loadings and the control were analytically verified via ICP-OES at the start (0 hours) and at the end of the exposure (72 hours) without algae.
Sampling and pre-treatment
At the start and at the end of the exposure phase, samples were taken from one additional replicate of each test item loading and the control without algae. These replicates were incubated under test conditions until sampling. 50 mL of each loading rate and the control were filled into a glass vial. All samples were taken in duplicate. The samples were stored cool in a fridge (2 – 8 °C) at the test facility. The first replicate was shipped pooled to the test site at the end of the exposure phase for analysis. The second replicate was stored cool in a fridge (2 – 8 °C) at the test facility. The principle investigator was informed by the study director prior to shipment. The study monitor was informed about the shipment. Analysis was performed as described in the phase plan. The analytical method, the results of the method validation as well as the results of the measured test samples were added to the final report. - Vehicle:
- no
- Details on test solutions:
- Preparation of the Saturated Solution
A saturated solution with a nominal loading rate of 100 mg test item/L was prepared once at room temperature 96 ± 2 hour prior to the start of the exposure. An appropriate amount of the test item was weighed out and transferred into a glass flask with an appropriate amount of demineralized water. The saturated solution was stirred with approximately 1100 rpm for 96 ± 2 hours at room temperature. After a separation phase of 30 minutes, the saturated solution was removed by siphoning from the approximate center of the glass bottle. Undissolved particles were removed by membrane filtration (membrane filter 0.45 µm, RC, MACHEREY-NAGEL). The filter was saturated in order to avoid adsorption during the filtration. The first 25 mL of the filtrate were discarded. The filtration was interrupted for 15 minutes to allow for adsorption and saturation of the filter material with dissolved test item. Thereafter, the filtration was continued. The next 25 mL were discarded. The following filtrate, i.e. the saturated solution, were used in the test. During filtration, the filter was always kept covered. The saturated solution was checked via laser beam (Tyndall effect) for undissolved test item. The Tyndall effect was negative.
After measurement of the Tyndall the components of the dilution water were added to the saturated solution.
Preparation of the test item solutions
Out of the saturated solution 3 concentrations were tested in a geometric series with a dilution factor of √10: 10.0 – 31.6 – 100% of the saturated solution.
The dilution levels are based on the results of a preliminary range finding test (non-GLP).
Control
Six replicates of dilution water (see above) without test item were exposed under the same conditions as the test groups. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Test organism Pseudokirchneriella subcapitata HINDÁK CCAP 278/4 (axenic)
Synonyms
Selenastrum capricornutum; Ankistrodesmus subcapitata; Raphidocelis subcapitata; Ankistrodesmus bibraianus (Experimental Phycology and Culture Collection of Algae at the University of Goettingen 2014)
Reason for the selection of the test organism
Pseudokirchneriella subcapitata is a suitable green alga species according to the guideline.
Origin
Culture Collection of Algae and Protozoa (CCAP)
SAMS Research Services Ltd
Dunstaffnage Marine Laboratory
Dunbeg, OBAN; Argyll PA37 1QA; Scotland, UK
Cultivation at test facility
Fresh stocks are prepared every month on Z-Agar. Light intensity amounts 2567 – 5130 lux for 24 hours per day.
Culture medium Nutrient medium Z according to LÜTTGE et al. (1994) - Test type:
- static
- Water media type:
- freshwater
- Remarks:
- According to the guidelines without the chelating agent Na2EDTA 2 H2O, due to the chemical properties of the test item
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- see section "Any other information on results incl. tables" below.
- Test temperature:
- see section "Any other information on results incl. tables" below.
- pH:
- see section "Any other information on results incl. tables" below.
- Dissolved oxygen:
- see section "Any other information on results incl. tables" below.
- Conductivity:
- see section "Any other information on results incl. tables" below.
- Nominal and measured concentrations:
- A saturated solution with a nominal loading rate of 100 mg test item/L was prepared. Out of the saturated solution 3 concentrations were tested in a geometric series with a dilution factor of √10: 10.0 – 31.6 – 100% of the saturated solution.
- Details on test conditions:
- Test method Static procedure
Duration of the test 72 hours
Replicates Six replicates for the control, three replicates per loading rate
Test container
Sterile Erlenmeyer flasks, volume: 250 mL, sealed with cotton wool plugs.
Test volume 100 mL
Ultrapure water
Ultrapure water was used to prepare the dilution water (conductivity max. 0.1 µS/cm).
Dilution water According to the guidelines without the chelating agent
Na2EDTA 2 H2O, due to the chemical properties of the test item.
Preculture
A four days old preculture, prepared in dilution water, was used as inoculum.
Initial cell density
Nominal: approximately 5 x 103 - 104 cells/mL
Actual: 6864 cells/mL
Application
Application was carried out by adding an appropriate amount of algae inoculum to the test solutions.
Incubation The flasks were positioned randomly and repositioned daily.
Temperature Nominal range: 21 - 24 °C, controlled at ± 2 °C
Agitation
Test containers were placed on a rotary shaker and oscillated at approximately 70 rpm.
Light intensity (target)
Approximately 4440 to 8880 lux, corresponding to 60 to 120 µE*m-2*s-1
Light regime 24 hours/day light
Light homogeneity Within ± 15% over incubation area
Type and Frequency of Measurements
Biological Parameters
Chlorophyll a-fluorescence
The cell density was measured daily via Chlorophyll a-fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as a background signal. No self-fluorescence was observed in the preliminary range finding test at the saturated solution (100 mg/L).
Microscopic evaluation
The algae cells were evaluated microscopically at the start and the end of the incubation period. The cells were checked for unusual cell shapes, colour differences, differences in chloroplast morphology, flocculation, adherence of algae to test containers and agglutination of algae cells.
Physico-chemical Properties
The pH-value at the start of the exposure was measured in one additional replicate of each test item loading rate and the control. At the end of the exposure, it was measured in a pooled sample of the test item loading rates and the control. The room temperature was measured continuously. Light intensity was measured prior to the start of the test. - Reference substance (positive control):
- yes
- Remarks:
- The toxicity of potassium dichromate (SIGMA ALDRICH, batch number BCCC1619, purity 100.0%, CAS RN 7778-50-9) to the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined over a period of 72 hours from 2021-11-01 to 2021-11-04.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Remarks:
- calculated nominal loading from a saturated solution of 100 mg/L
- Effect conc.:
- 73.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL20
- Remarks:
- calculated nominal loading from a saturated solution of 100 mg/L
- Effect conc.:
- 32.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Remarks:
- calculated nominal laoding from a saturated solution of 100 mg/L
- Effect conc.:
- 15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOELR
- Remarks:
- calculated nominal loading from a saturated solution of 100 mg/L
- Effect conc.:
- 31.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Remarks:
- calculated nominal loading from a saturated solution of 100 mg/L
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Remarks:
- calculated nominal loading from a saturated solution of 100 mg/L
- Effect conc.:
- 56.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOELR
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 31.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- calculated nominal loading from a saturated solution of 100 mg/L
- Basis for effect:
- growth rate
- Details on results:
- Biological Data
Microscopic evaluation of the cells at the start and the end of exposure revealed no morphological abnormalities. All effect values given are based on the nominal test item loading rates of the test item Mo10V3TeNbO42.
Physico-chemical Data
The environmental conditions (pH-value, room temperature, light intensity) were determined to be within the acceptable limits. The test media were visually clear throughout the exposure period (possible turbidity related to algae growth not taken into account). - Results with reference substance (positive control):
- The toxicity of potassium dichromate (SIGMA ALDRICH, batch number BCCC1619, purity 100.0%, CAS RN 7778-50-9) to the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined over a period of 72 hours from 2021-11-01 to 2021-11-04. The reference item toxicity is in the valid range which was established by calculation of the average of the historic reference data since 2006, and the limits were set using the threefold standard deviation of these values
- Reported statistics and error estimates:
- EL-values and statistical analyses
EL10-,EL20- and EL50- values of growth rate inhibition and for yield inhibition after 72 hours were calculated by sigmoidal dose-response regression. The respective 95% confidence limits were calculated from the standard error and the t distribution wherever possible. In the case that the confidence limits could not be calculated by the software, the respective confidence limits were empirically derived from the observation data. All calculations were carried out from the best-fit values with the software GraphPad Prism.
NOEL, LOEL and The NOEL / LOEL was determined by calculation of statistical
statistical analyses significant differences of growth rate and yield using Dunnett’s Method. A Shapiro-Wilk’s Normality test and a Brown-Forsythe’s Equal Variance test were done first. P-values for both Normality and Equal Variance tests are 0.05. The -value (acceptable probability of incorrectly concluding that there is a difference) is = 0.01.
Software The data for the tables in this report were computer-generated and have been rounded for presentation from the full derived data. Consequently, if calculated manually based on the given data minor deviations may occur from these figures.
Calculations were carried out using software
• Excel, MICROSOFT CORPORATION
• SigmaPlot, SPSS INC.
• GraphPad Prism, GRAPHPAD SOFTWARE, INC. - Validity criteria fulfilled:
- yes
- Conclusions:
- In this study, Mo10V3TeNbO42 was found to inhibit the growth of the freshwater green alga Pseudokirchneriella subcapitata after 72 hours with the following effect values (based on the nominal loading rate of the test item): The EL50-values for inhibition of growth rate (ErL50) and yield (EyL50) after 72 hours were > 100 mg/L and 73.4 mg/L (CI: 31.6 – 100 mg/L), respectively. The NOEL-values for both inhibition of growth rate and yield after 72 hours were 31.6 mg/L and 10.0 mg/L.
- Executive summary:
The toxicity of Mo10V3TeNbO42 to the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined according to the principles of OECD 201 and Council Regulation (EC) No. 266/2016/Method C.3 from 2021-09-24 to 2021-11-18, with the definitive exposure phase from 2021-11-09 to 2021-11-12 at the test facility. The aim of the study was the determination of the effects on growth rate and yield over a period of 72 hours.
The study was conducted under static conditions with an initial cell density of 6864 cells/mL. A saturated solution was prepared as described with demineralized water 96 ± 2 hour prior to the start of the exposure. The test media were visually clear throughout the test period. Three test loadings were tested in a geometrical series with a dilution factor of √10: 10.0 - 31.6 - 100% of the saturated solution. Three replicates were tested for each test item loading and six replicates for the control. The environmental conditions were within the acceptable limits.
The molybdenum concentration was determined via element analysis of Mo, processed at the Test Site (WeylChem InnoTec GmbH). All loading rates of the test item and the control were analysed via ICP-OES at the start (0 hours) and at the end of the exposure (72 hours) without algae. The analytical method, the results of the method validation as well as the results of the measured test samples were added to the final report. For details, see phase report in Annex I.
NOEL, LOEL and ELx-values (and Confidence Intervals empirically derived from the observation data) of Mo10V3TeNbO42 (0 - 72 hours)
based on the nominal loading rates of the test item [mg/L]Inhibition of Growth Rate (Nominal loading rates of the test item [mg/L]) NOEL 31.6* LOEL 100 ErL10 56.3* (CI: 31.6 – 100) ErL20 > 100 ErL50 > 100 Inhibition of Yield (Nominal loading rates of the test item [mg/L]) NOEL 10.0* LOEL 31.6* EyL10 15.0* (CI: ≤10 – 31.6) EyL20 32.6* (CI: 10 – 100) EyL50 73.4* (CI: 31.6 – 100) *calculated nominal loading from a saturated solution of 100 mg/L
Reference
Cell Densities
Saturated solution with a nominal loading of | Replicate | Cell density [cells/mL] | |||
100 mg/L | |||||
[%] | No. | 0 hours | 24 hours | 48 hours | 72 hours |
100 | 1 | 6864 | 22762 | 118288 | 528060 |
2 | 6864 | 24440 | 120852 | 454203 | |
3 | 6864 | 19269 | 106206 | 490277 | |
Mean | 6864 | 22157 | 115115 | 490847 | |
31.6 | 1 | 6864 | 21808 | 144934 | 1010943 |
2 | 6864 | 29603 | 144988 | 1030195 | |
3 | 6864 | 29932 | 187332 | 947521 | |
Mean | 6864 | 27114 | 159085 | 996220 | |
10.0 | 1 | 6864 | 26057 | 166669 | 1185960 |
2 | 6864 | 26115 | 153058 | 1048232 | |
3 | 6864 | 27370 | 181476 | 1139451 | |
Mean | 6864 | 26514 | 167068 | 1124548 | |
Control | 1 | 6864 | 28812 | 162773 | 1258693 |
2 | 6864 | 25242 | 159688 | 1268229 | |
3 | 6864 | 30465 | 195348 | 1286221 | |
4 | 6864 | 28742 | 174594 | 1298815 | |
5 | 6864 | 26772 | 201672 | 1103332 | |
6 | 6864 | 27174 | 177275 | 1175840 | |
Mean | 6864 | 27868 | 178558 | 1231855 |
Evaluation after 72 hours
Statistically significant differences of growth rates and yield compared to
control values are marked (s), not significant differences are marked (ns).
Saturated solution with a nominal loading of | Replicate | Growth rate | Inhibition of growth rate | Yield | Inhibition of yield | ||
100 mg/L | |||||||
[%] | No. | [d-1] | [%] | [cells/mL] | [%] | ||
100 | 1 | 1.45 | 16 | 521196 | 57 | ||
2 | 1.40 | 19 | 447339 | 63 | |||
3 | 1.42 | 18 | 483413 | 61 | |||
Mean | (s) | 1.42 | 18 | (s) | 483983 | 60 | |
31.6 | 1 | 1.66 | 4 | 1004079 | 18 | ||
2 | 1.67 | 3 | 1023331 | 16 | |||
3 | 1.64 | 5 | 940657 | 23 | |||
Mean | (s)* | 1.70 | 4 | (s) | 989356 | 19 | |
10.0 | 1 | 1.72 | 1 | 1179096 | 4 | ||
2 | 1.68 | 3 | 1041368 | 15 | |||
3 | 1.70 | 1 | 1132587 | 8 | |||
Mean | (ns) | 1.70 | 2 | (ns) | 1117684 | 9 | |
Control | 1 | 1.74 | 1251829 | ||||
2 | 1.74 | 1261365 | |||||
3 | 1.74 | 1279357 | |||||
4 | 1.75 | 1291951 | |||||
5 | 1.69 | 1096468 | |||||
6 | 1.71 | 1168976 | |||||
Mean | 1.73 | 1224991 |
* = statistical significant but biologically not relevant (< 5%)
Section-by-Section and Average Specific Growth Rates of the Control Group
(0 - 72 hours)
Replicate No. | Specific Growth Rate [d-1] | Mean | SD | CV | Mean CV [%] | |||
section-by-section | (0 - 72 hours) | ± | [%] | |||||
0 - 24 hours | 24 - 48 hours | 48 - 72 hours | ||||||
Control | 1 | 1.44 | 1.73 | 2.05 | 1.74 | 0.306 | 17.6 | 17.7 |
2 | 1.30 | 1.85 | 2.07 | 1.74 | 0.396 | 22.7 | ||
3 | 1.49 | 1.86 | 1.89 | 1.74 | 0.221 | 12.6 | ||
4 | 1.43 | 1.80 | 2.01 | 1.75 | 0.292 | 16.7 | ||
5 | 1.36 | 2.02 | 1.70 | 1.69 | 0.329 | 19.4 | ||
6 | 1.38 | 1.88 | 1.89 | 1.71 | 0.293 | 17.1 | ||
Mean | 1.73 | |||||||
SD ± | 0.02 | |||||||
CV [%] | 1.23 |
SD = Standard deviation CV = Coefficient of variation
Environmental Conditions
Saturated solution with a | pH-values | ||||||
nominal loading of 100 mg/L | |||||||
[%] | Start; 0 hours | End; 72 hours | |||||
100 | 7.91 | 8.22 | |||||
31.6 | 7.97 | 8.64 | |||||
10.0 | 8.05 | 8.78 | |||||
Control | 8.03 | 8.98 | |||||
Room temperature [°C]: | min.: 22.0 | max.: 22.5 | mean value: 22.3 | ||||
n = 24 | mean value: 5436 | CV [%]: 6.73 | |||||
Light intensity | range of the measured values: 4671 - 6005 | ||||||
[lux] | equalling -14.1 to 10.5 |
CV = Coefficient of variation n = number of measuring points
Water parameters of the Dilution Water
Parameters of the dilution water (measured on 2021-11-09) | ||||
Conductivity | Total hardness | Acidity | Alkalinity | Total organic carbon |
[µS/cm] | [mg CaCO3/L] | [mmol/L] | [mmol/L] | [mg C/L] |
136 | 40 | 0.2 | 0.6 | < LOQ* |
*Limit of quantification = 2.00 mg C/L
Validity Criteria
Validity Criterion | Required | This study |
Increase of the cell growth in the control cultures | Exponentially, ≥ 16-fold corresponding to a specific growth rate of 0.92 day-1 | 179-fold |
(specific growth rate 1.70 day-1) | ||
Mean coefficients of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3) in the control cultures | ≤ 35% | 17.7% |
Coefficient of variation of average specific growth rates during the whole test period in replicate control cultures | ≤ 7% | 1.23% |
Results
The purpose of this study was the determination of the concentration of molybdenum in test medium with dissolved test item Mo10V3TeNbO42 prepared by Noack Laboratorien (study no. SO21063 / SPO19517). Therefore, the provided sample solutions were acidified and analysed by inductively coupled plasma and optical emission spectrometry (ICP-OES).
The validity of the applied analytical method was proven (specificity, linearity, limit of quantification (LOQ), accuracy and precision). The validation results meet the specifications according to SANTE/2020/12830, Rev.1.
The mean results of the provided sample solutions are summarized in the following table:
Date | Sample | Sample description Nominal Sample concentration (mg test item/L) | Mean measured | Recovery1) |
code | Mo concentration | |||
[yyyy-mm-dd] | [mg/L] | [%] | ||
30.09.2021 | - | Test medium | < 0.01 | - |
28.09.2021 | A0 | Control | < 0.01 | - |
29.09.2021 | E0 | Control | < 0.01 | - |
28.09.2021 | A0 | 10 | 0.2701 | 5.64 |
29.09.2021 | E0 | 10 | 0.2635 | 5.50 |
28.09.2021 | A0 | 31.6 | 0.829 | 5.48 |
29.09.2021 | E0 | 31.6 | 0.845 | 5.59 |
28.09.2021 | A0 | 100 | 2.63 | 5.49 |
29.09.2021 | E0 | 100 | 2.65 | 5.54 |
1) Mo recovery related to Mo content (47.87%, stoichiometric) of the test item
1st Level (0.03 mg/L, LOQ level):
Data for the evaluation of the recovery and repeatability of Mo concentration 1st level using spiked matrix samples (0.03 mg/L)
Validation | Nominal Mo | Intensity, mean | Intensity value 1 | Intensity value 2 | Intensity value 3 |
sample no. | concentration | ||||
[mg/L] | [counts/s] | [counts/s] | [counts/s] | [counts/s] | |
1 | 0.03 | 23.01 | 23.06 | 22.64 | 23.32 |
2 | 0.03 | 24.67 | 24.14 | 25.34 | 24.52 |
3 | 0.03 | 24.14 | 25.13 | 22.99 | 24.31 |
4 | 0.03 | 24.54 | 24.82 | 24.79 | 24.01 |
5 | 0.03 | 23.28 | 23.07 | 23.76 | 23.00 |
Validation | Nominal Mo | Measured Mo | Uncorrected recovery | Matrix effect | Corrected recovery |
sample no. | concentration | concentration | |||
[mg/L] | [mg/L] | [%] | [%] | [%] | |
1 | 0.03 | 0.03639 | 121.30 | 14.71 | 106.59 |
2 | 0.03 | 0.03880 | 129.33 | 22.98 | 106.35 |
3 | 0.03 | 0.03803 | 126.77 | 20.34 | 106.43 |
4 | 0.03 | 0.03861 | 128.70 | 22.33 | 106.37 |
5 | 0.03 | 0.03678 | 122.60 | 16.05 | 106.55 |
Mean | 106.46 | ||||
SD | 0.11 | ||||
%RSD | 0.10 |
2nd Level (0.30 mg/L, 10 x LOQ level):
Data for the evaluation of the recovery and repeatability of Mo concentration 2nd level
using spiked matrix samples (0.30 mg/L)
Validation | Nominal Mo | Measured Mo | Recovery | Intensity mean | Intensity value 1 | Intensity value 2 | Intensity value 3 |
sample no. | concentration | concentration | |||||
[mg/L] | [mg/L] | [%] | [counts/s] | [counts/s] | [counts/s] | [counts/s] | |
6 | 0.30 | 0.30565 | 101.88 | 208.8 | 207.2 | 209.2 | 210.1 |
7 | 0.30 | 0.30420 | 101.40 | 207.8 | 206.9 | 208.5 | 208.2 |
8 | 0.30 | 0.30087 | 100.29 | 205.5 | 207.0 | 205.1 | 204.3 |
9 | 0.30 | 0.30507 | 101.69 | 208.4 | 208.5 | 207.1 | 209.6 |
10 | 0.30 | 0.30710 | 102.37 | 209.8 | 209.4 | 209.1 | 211.0 |
Mean | 101.53 | ||||||
SD | 0.78 | ||||||
%RSD | 0.77 |
Description of key information
In this study, Mo10V3TeNbO42 was found to inhibit the growth of the freshwater green alga Pseudokirchneriella subcapitata after 72 hours with the following effect values (based on the nominal loading rate of the test item): The EL50-values for inhibition of growth rate (ErL50) and yield (EyL50) after 72 hours were > 100 mg/L and 73.4 mg/L (CI: 31.6 – 100 mg/L), respectively. The NOEL-values for both inhibition of growth rate and yield after 72 hours were 31.6 mg/L and 10.0 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 31.6 mg/L
Additional information
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