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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 5, 2020 to September 17, 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Sulfonic acids, C16-alkane hydroxy and C16-alkene, potassium salts
- Cas Number:
- 2210238-42-7
- IUPAC Name:
- Sulfonic acids, C16-alkane hydroxy and C16-alkene, potassium salts
- Test material form:
- cream / paste
- Remarks:
- This sample was obtained from the original liquid form by removing water.
- Details on test material:
- Identification: Internal Olefin Sulfonate
Description: pale yellow paste
Constituent 1
- Specific details on test material used for the study:
- Expiry date: January 24, 2023
Storage conditions: room temperature in the dark, kept in airtight container
Storage place: desiccator
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, non-adapted
- Details on inoculum:
- Inoculum: Activated sludge from domestic sewage treatment plant
Source: Sewage treatment plant mainly handling domestic wastewater in Kanagawa
Date of collection: June 4, 2020
Culture condition: The inoculum was maintained in an aerobic state until used in the test.
Mixed liquor suspended solids (MLSS): 1600 mg/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 30 mg/L
- Based on:
- act. ingr.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- Degradability study conditions
Apparatus
Thermostatic water bath: Water bath shaker MM-10, Taitec Corp.
Conditions
Temperature: 22 土 2°c
Exposure period: 28 days
Test vessel: 1 L Erlenmeyer flask, glass (covered with aluminum foil)
Test volume: 500 mL
Concentration:
Test substance: 30.0 mg/L
Reference substance: 60.0 mg/L
Inoculum: 30 mg/L
Light: Fluorescent light
Test bottle contents and preparation of test solutions
Outline of the test solution was shown below. The test substance solution*1 or reference substance solution*2 were added to the mineral medium in test vessels. Then inoculum was added after the pH was measured.
pH meter: F-52, HORIBA, Ltd.
*1 Preparation of test substance solution:
In ultra-pure water, 82.0 mg of the test sample (50.0 mg as the test substance after purity correction) was dissolved by ultrasonication for approximately 1 minute, and filled up to 50 mL to make a test substance solution of 1000 mg/L.
*2 Preparation of reference substance solution:
In ultra-pure water, 100 mg of the reference substance was dissolved, and filled up to 100 mL to make a reference substance solution of 1000 mg/L.
Bottle No.1 and 2 (Test suspension-1 and 2)
test substance solution 15 mL, mineral medium 500 mL*3, Inoculum 9.4 mL*4
Bottle No.3 (Procedure control)
reference substance solution 30 mL, mineral medium 500 mL*3, Inoculum 9.4 mL*4
Bottle No.4 and 5 (Inoculum blank-1 and 2)
mineral medium 500 mL*3, Inoculum 9.4 mL*4
*3 The total volume of the test substance solution or reference substance solution and the inoculum was held fixed at 500 mL.
*4 The inoculum addition= 30 (mg/L) + MLSS (mg/L) x 500 (mL)
Management during exposure period
At the start and end of exposure, the water temperature in the thermostatic water bath was measured with the following apparatus. During the exposure period, the test solution volume, the set temperature of the thermostatic bath, and that the test vessel was being shaken under fluorescent light were checked once a day except on holidays when no sampling was performed. At the end of exposure, the dissolved oxygen concentration (DO) of the test solution was measured with the following apparatus to confirm that the test solution was aerobic.
Apparatus
Thermo recorder: Wireless RTR-71(No.4), T&D
DO meter: 5100(No.1), YSI/Nanotech Inc.
Evaluation of test results
Equations for calculation of degradability
Degradability based on DOC was calculated for each bottle by the following equation.
Degradability (%) = [1 - (DOCs-DOCb) / (DOCs0-DOCb0)] x 100
Where DOCs: DOC (mgC/L) in the [Inoculum + test substance] or the [Inoculum +
reference substance] at day x
DOCb: Average of DOC (mgC/L) in the [Control blank] at day x
DOCsO: DOC (mgC/L) in the [Inoculum + test substance] or the [Inoculum +
reference substance] at day 0
DOCbO: Average of DOC (mgC/L) in the [Control blank] at day 0
Judgment of degradability
The pass levels for readily degradability were shown below.
· Degradability based on DOC is 70% or more.
· The pass value (the attainment of 70% biodegradation) has to be reached in a 10-d
window*1 within the 28-d period of the test. The 10-d window begins when the degree of
biodegradation has reached 10% DOC and must end before day 28 of the test.
*1 The 10 days immediately following the attainment of 10% biodegradation.
Validity of the test
The test was considered valid if the following criteria were met.
1) The degradability of reference substance based on the DOC exceeds 70% after 14 days.
2) The difference between the maximum and minimum values of the degradability in the
[Inoculum + test substance] is less than 20%.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- All of the validity criteria given in the guideline were fulfilled and hence the test was judged valid.
% Degradation
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 97
- Sampling time:
- 28 d
- Details on results:
- Factors which might have affected the reliability of the test results
There was no specific factor which might have affected the reliability of the test results.
DO measurement
At the end of exposure, the DO values were 8.83 and 8.90 mgO2/L for the [Inoculum + test substance]-1 and 2 (bottle 1 and 2), 8.84 and 8.86 mgO2/L for the [Control blank]-1 and 2 (bottle 4 and 5), 8.81 mgO2/L for the [Inoculum + reference substance] (bottle3), respectively.
Degradability based on the DOC
The DOC in the [Inoculum + test substance]-! and 2 at day 28 were 1.7 and 2.0 mgC/L, and the DOC in the [Control blank]-1 and 2 were 1.2 and 1.3 mgC/L. The degradabilities based on the DOC at day 28 were calculated to be 97% and 96% for the [Inoculum + test substance] -1 and 2, respectively.
The degradabilities based on the DOC at day 14 was calculated to be 97% for the [Inoculum + reference substance].
Any other information on results incl. tables
Result of DOC measurement (mgC/L)
Bottle No. | Sample description | Day | ||||||
0 | 5 | 7 | 14 | 21 | 28 | |||
1 | Test suspension-1 [Inoculum+test substance]-1 | a1 | 17.16 | 8.92 | 4.59 | 2.95 | 2.62 | 1.66 |
a2 | 17.16 | 8.72 | 4.65 | 3.01 | 2.63 | 1.79 | ||
Ave., A | 17.2 | 8.8 | 4.6 | 3.0 | 2.6 | 1.7 | ||
2 | Test suspension-2 [Inoculum+test substance]-2 | b1 | 17.21 | 9.67 | 4.76 | 2.95 | 2.14 | 1.82 |
b2 | 17.39 | 9.90 | 4.69 | 3.01 | 2.23 | 2.09 | ||
Ave., B | 17.3 | 9.8 | 4.7 | 3.0 | 2.2 | 2.0 | ||
3 | Procedure control [Inoculum+reference substance] | p1 | 36.88 | 3.30 | 3.78 | 2.22 | 2.03 | 1.97 |
p2 | 36.91 | 3.55 | 3.65 | 2.20 | 2.10 | 2.18 | ||
Ave., P | 36.9 | 3.4 | 3.7 | 2.2 | 2.1 | 2.1 | ||
4 | Inoculum blank-1 [Control blank]-1 | c1 | 1.25 | 1.04 | 1.31 | 0.89 | 0.94 | 1.14 |
c2 | 1.27 | 1.44 | 1.54 | 1.18 | 1.24 | 1.29 | ||
Ave., C | 1.3 | 1.2 | 1.4 | 1.0 | 1.1 | 1.2 | ||
5 | Inoculum blank-2 [Control blank]-2 | d1 | 1.26 | 1.41 | 1.33 | 0.99 | 1.07 | 1.18 |
d2 | 1.31 | 1.75 | 1.44 | 1.22 | 1.06 | 1.34 | ||
Ave., D | 1.3 | 1.6 | 1.4 | 1.1 | 1.1 | 1.3 | ||
|
| Ave. (C, D), I | 1.3 | 1.4 | 1.4 | 1.1 | 1.1 | 1.3 |
Degradability (%)
Bottle No. | Sample description | Day | ||||||
0 | 5 | 7 | 14 | 21 | 28 | |||
1 | Test suspension-1 [Inoculum+test substance]-1 | D1 | 0 | 53 | 80 | 88 | 91 | 97 |
2 | Test suspension-2 [Inoculum+test substance]-2 | D2 | 0 | 48 | 79 | 88 | 93 | 96 |
|
| Ave., (D1, D2) | 0 | 51 | 80 | 88 | 92 | 97 |
3 | Procedure control [Inoculum+reference substance] | D3 | 0 | 94 | 94 | 97 | 97 | 98 |
Equations:
D1 = [1 - (Ax - Ix) / (A0 - I0)] × 100
D2 = [1 - (Bx - lx) / (B0 - I0 )] × 100
D3 = [1 - (Px - Ix) / (P0 - I0 )] × 100
Ax, Bx, Px: DOC (mgC/L) in the [Inoculum + test substance] or the [Inoculum + reference substance] at day x
Ix: Average of DOC (mgC/L) in the [Control blank] at day x
A0, B0, P0: DOC (mgC/L) in the [Inoculum + test substance] or the [Inoculum + reference substance] at day 0
I0: Average of DOC (mgC/L) in the [Control blank] at day 0
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The pass value of 70% biodegradation was considered to be reached at the end of the 10-d window because the biodegradation rate based on the DOC had exceeded 70% at day 7 (Average value: 80%). As the biodegradation rate was over 70% at the end of the 10-d window and the end of the exposure (day 28), these results indicate that the test substance was readily biodegradable under the test conditions.
- Executive summary:
METHOD
OECD Guideline for Testing of Chemicals 301A "Ready Biodegradability: DOC Die-Away Test" (1992)
(Study period)
June 5, 2020 to September 17, 2020
(Test bottle contents)
Bottle 1, 2 Test suspension: [Inoculum + test substance]-1, 2
Bottle 3 Procedure control: [Inoculum + reference substance]
Bottle 4, 5 Inoculum blank: [Control blank]-1, 2
(test substance: 30.0 mg/L, reference substance: 60.0 mg/L, inoculum: 30 mg/L)
(Measurements)
Dissolved organic carbon (DOC) [measured with a TOC analyzer]
RESULTS
Measured values after 28 days
Measurement
[Inoculum +test substance]
[Control blank]
1
2
1
2
DOC, mgC/L
1.7
2.0
1.2
1.3
Degradability, %
97
96
-
-
CONCLUSION
It is concluded that the test substance is readily biodegradable under the conditions of this test.
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