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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- other: read across from supporting substance
- Adequacy of study:
- key study
- Study period:
- Since October 1,1985 to October 25,1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- Deviations:
- no
- GLP compliance:
- yes
- Radiolabelling:
- no
- Analytical monitoring:
- no
- Buffers:
- - pH:4, 7 , 9
- Type and final molarity of buffer: 0.05 mol/l
- Composition of buffer:
Buffer solution pH 4: Potassium in dist. water,
(C6H4 (000H) COOK) adjusted = 0.05 mol / l sodium hydroxide added to pH 4.0
Buffer solution pH 7: potassium hydroxide and water,
(KH2P04) = 0.05 mol / l, adjusted to pH 7.0 by sodium hydroxide addition
Buffer solution pH 9 boric acid in aqueous KCl solution
(KCl) = 0.05 mol / l
(H3BO3) = 0.05 mol / l,
adjusted by sodium hydroxide added to pH 9.0 - Details on test conditions:
- TEST SYSTEM
- Type, material and volume of test flasks, other equipment used: 100 ml flask
- Sterilisation method: yes , boiling glassware for 30 minute and after at 180°C (in oven) for 2 hours
TEST MEDIUM
- Preparation of test medium:
c.a 0.2 ± 1g of item substance was dissolved in 100 ml of buffer (the buffers used was been at an pH=4, pH=7 and pH=9).
In flasks, 10.0 ml of this latter solution were diluited to 100 ml with same buffers (pH 4, 7 and 9).
The flaskes were put in an thermostated bath and before the HPLC analisy, rapidily cooled in an iced bath.
OTHER TEST CONDITIONS
- Adjustment of pH: yes
- concentration was 0.2 ± 1 g / L, on the pure dye converted to about 0.17 g / L, or about 3 x 10^-4 mol / L. - Duration:
- 5 d
- pH:
- 4
- Temp.:
- 25 °C
- Initial conc. measured:
- ca. 0.2 g/L
- Duration:
- 5 d
- pH:
- 7
- Temp.:
- 38 °C
- Initial conc. measured:
- ca. 0.2 g/L
- Duration:
- 5 d
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- ca. 0.2 g/L
- Duration:
- 5 d
- pH:
- 9
- Temp.:
- 25 °C
- Initial conc. measured:
- ca. 0.2 g/L
- Positive controls:
- not specified
- Negative controls:
- not specified
- Transformation products:
- no
- % Recovery:
- ca. 98.5
- pH:
- 4
- Temp.:
- 25 °C
- Duration:
- ca. 2.4 h
- % Recovery:
- ca. 98
- pH:
- 4
- Temp.:
- 25 °C
- Duration:
- ca. 5 d
- % Recovery:
- ca. 70
- pH:
- 7
- Temp.:
- 25 °C
- Duration:
- ca. 2.4 h
- % Recovery:
- < 1
- pH:
- 7
- Temp.:
- 25 °C
- Duration:
- ca. 5 d
- % Recovery:
- < 1
- pH:
- 9
- Temp.:
- 25 °C
- Duration:
- ca. 2.4 h
- % Recovery:
- < 1
- pH:
- 9
- Temp.:
- 25 °C
- Duration:
- ca. 5 d
- pH:
- 7
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- ca. 0.116 h-1
- DT50:
- ca. 6 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 7
- Temp.:
- 38 °C
- Hydrolysis rate constant:
- ca. 0.027 h-1
- DT50:
- ca. 25.7 h
- Type:
- (pseudo-)first order (= half-life)
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on results of test item at pH 4,7(at 38 and 50°C) and 9 ,the substance has a t1/2 of about 6 days at 25°C
- Executive summary:
The substance is tested by EU method C.7 hydrolisis as function of pH. The substance has been tested at ph buffer 4, 7 ,9. Based on results of test item at pH 4, 7(at 38 and 50°C) and 9 , the substance has a calculated t1/2 of about 6 days at 25°C.
Reference
log K= -A/T + B
A= 5284
B=15.4
Kobs= 0.0049 h^-1
t1/2(ph7) at 25°C ~ 6 d
test 1 (50°C, pH=7)
time | c (0) | c (t) | c(t)/c(0) | - Log c(t)/c(0) |
2 | 0.2001 | 0.1583 | 0.7909 | 0.1019 |
3 | 0.2001 | 0.1413 | 0.7062 | 0.1511 |
4 | 0.2001 | 0.1261 | 0.6304 | 0.2004 |
6 | 0.2001 | 0.1007 | 0.5035 | 0.298 |
8 | 0.2001 | 0.079 | 0.3949 | 0.4035 |
test 3 (38°C, pH=7)
time | c (0) | c (t) | c(t)/c(0) | -Log c(t)/c(0) |
1.33 | 0.201 | 0.192 | 0.9551 | 0.01995 |
21 | 0.2 | 0.1136 | 0.5681 | 0.2455 |
24 | 0.2006 | 0.1034 | 0.5155 | 0.2878 |
27 | 0.2006 | 0.0968 | 0.4825 | 0.3165 |
31 | 0.2006 | 0.0877 | 0.437 | 0.3595 |
Description of key information
EUC7, similar substance 1, DT50 = 6 days at 25°C, pH 7
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 6 d
- at the temperature of:
- 25 °C
Additional information
Reactive Orange 16 similar substance 1 is tested by EU method C.7 hydrolysis as function of pH. The substance has been tested at ph buffer 4, 7 ,9. Based on results of test item at pH 4,7 (at 38 and 50°C) and 9 , the reactive orange 16 had shown a t1/2 of about 6 days at 25°C. (Bartsch 1985)
In the preliminary experiments with similar substance 2 at pH 7 and 50°C , was observed that in 2.4 hours the presence of the vinyl branch "[[4-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]azo]" were less than 1%. (Appel 1989) This part of molecule is identical to reactive orange 16 and reactive orange 16 similar substance 1, in fact the two reactive orange 16 differ only for the counter ionic part (Lithium versus Sodium), this difference has no influence on the hydrolysis pathway. It is reasonable to suppose that the reaction is the same for reactive orange 16.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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