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EC number: 941-151-0 | CAS number: 1690331-63-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2014-08-05 to 2014-08-14
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The reliability is rated 2 because the study followed a standard guideline of reference (OECD 202), which describes a procedure designed to evaluate this endpoint. The results were reviewed for reliability and assessed as valid. The study was conducted under GLP conditions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- (since the test item is poorly water soluble, the standard method for the preparation of aqueous media was modified. The test was performed with a saturated solution of the test item)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- no
Test material
- Reference substance name:
- [bis(phenylamino)methylidene]azanium 3-[(1E)-2-[4-(phenylamino)phenyl]diazen-1-yl]benzene-1-sulfonate
- EC Number:
- 941-151-0
- Cas Number:
- 1690331-63-5
- Molecular formula:
- C31H28N6SO3
- IUPAC Name:
- [bis(phenylamino)methylidene]azanium 3-[(1E)-2-[4-(phenylamino)phenyl]diazen-1-yl]benzene-1-sulfonate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): Yellow MG-DPG
- Substance type: organic salt
- Physical state: powder
- Stability under test conditions: stable
- Storage condition of test material: ????
- Other: solubility = 140 mg/L
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method of preparation: Saturation of the experimental solution:
100 mg of test item was dispersed in 1 liters test medium
Stirring for 24 hours.
Filtration through a PTFE of 0.22 μm to remove undissolved test item
Obtention of a 100% v/v saturated solution.
Series of dilution to give further test concentration for the range finding study and the main test.
- Application: daphnies were placed in test tube containing 10 mL of the saturated solution test
- Controls: control group maintained under identical conditions but not exposed to the test item
- Vehicle: none
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: in-house subcontracting laboratory cultures
- Age at study initiation: less than 24 hours old
- Method of breeding:
Breeding medium: Adult Daphnia were maintained in a synthetic medium as defined by the standard NF EN ISO 6341
Temperature: 18°C
Continuous lightning (intensity of 1000 lux on the containers surface)
No aeration
Containers: 3 litre glass beaker
Breeding charge: around 50 spawners per glass beaker
Breeding medium renewal frequency: twice per week
Spawners renewal frequency: around every 3 weeks
Test medium acclimatation of the spawners: no period of acclimatation
- Feeding during test: no
ACCLIMATION
- Acclimation period: no
QUARANTINE (wild caught)
- Duration:no quarantine
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 225 mg/L +/- 50 mg/L as CaCO3
- Test temperature:
- 18°C to 22°C
- Nominal and measured concentrations:
- Nominal test concentration, in % v/v saturated solution :
a - control
b- 18 % v/v
c- 27 % v/v
d- 40 % v/v
e- 60 % v/v
f- 100 % v/v - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass test tube of 10 mL
- Type : closed
- Material, size, headspace, fill volume: 10 mL glass test tube
- Aeration: no
- Renewal rate of test solution: no renewal
- No. of organisms per vessel: 5 organisms
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 4 replicates
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: medium prepared according to the standard NF EN ISO 6341 (see table 2 for composition).
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continous lighting
- Light intensity: no light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- immobilisation of the daphnies
TEST CONCENTRATIONS
- Spacing factor for test concentrations: mean of √2.4
- Range finding study: yes
- Test concentrations: 18, 27, 40, 60 and 100 % v/v saturated solution
- Results used to determine the conditions for the definitive study: cumulative immobilization data from the range-finding study - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr207)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- ca. 27 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: (95% CL: 84.4 - > 100 mg/l)
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - Results with reference substance valid? yes. EC50 - 24 hr = 1.1 mg/L (2014/08/11)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the experimental conditions:
* 48h - EC50 > 100 mg/L
* 48h - NOEC = 27 mg/L
* 48h - LOEC > 100 mg/L - Executive summary:
Introduction
A study was performed to assess the acute toxicity of the test item to Daphnia magna.The method followed was designed to be compatible with OECD Guidelines. for Testing of Chemicals (April 2004) No 202,"Daphnia sp., Acute Immobilisation Test", referenced as method C.2 of Council Regulation (EC) No. 440/2008.
Methods
Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item at concentrations of 1.0, 3.5, 10, 35 and 100% v/v saturated solution for 48 hours at a temperature of 20°C to 21°C under static test conditions.
The test item solutions were prepared by stirring an excess of test item in test medium using a propeller stirrer at approximately 1500 rpm for 24 hours. After the stirring period any undissolved test item was removed by filtration (0.2 µm Gelman Acrocap filter) to produce a 100% v/v saturated solution of the test item. This saturated solution was then further diluted as necessary, to provide the remaining test groups at 18, 27, 40, 60 and 100 mg/L. The number of immobilized Daphnia were recorded after 24 and 48 hours.
Results
Exposure of Daphnia magna to the test item gave the following results based on the geometric mean measured test concentrations:
- EC50 – 48 hr > 100 mg/L (IC95%84.4 - > 100)
- NOEC = 27 mg/l
- LOEC > 100 mg/l
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