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Reaction mass of bis(polysubstituted hexanoic acid) bis(3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl) diphosphate and bis(polysubstituted hexanoic acid) 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl phosphate and polysubstituted hexanoic acid bis(3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl) hydrogen diphosphate and polysubstituted hexanoic acid bis(3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl) phosphate and polysubstituted hexanoic acid 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl hydrogen phosphate
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- Remarks:
- Study conducted according to guideline in effect at time of study conduct
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1300 (Daphnid Chronic Toxicity Test)
- Deviations:
- no
- Remarks:
- Study conducted according to guideline in effect at time of study conduct
- GLP compliance:
- yes
Test material
- Reference substance name:
- Phosphoric acid, mixed esters with partially fluorinated alcohol, amine salts
- IUPAC Name:
- Phosphoric acid, mixed esters with partially fluorinated alcohol, amine salts
- Details on test material:
- - Purity: 20.0% solids in water
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations Sampled: All test samples were analysed.
- Sampling Times: One sample plus a back-up sample from each test concentration, including the dilution water control, before and after addition of the diet solution, on day 0 and day 2 of the test before the test solutions were poured into the replicate test chambers. One sample plus a back-up sample from each test concentration, including the dilution water control, after addition of the diet solution, on days 9, 16, and 19 of the test before the test solutions were poured into the replicate test chambers. One sample plus a back-up sample from 2 of the 10 replicate chambers at each test concentration, including the dilution water control, on days 5, 12, 19 and 21 of the test. On day 19 test samples for the 0.63 mg total solids/L dose level from 2 chambers due to total adult immobility and on day 21 test samples for the 0.63 mg total solids/L and the 2.5 mg total solids/L dose level from 4 chambers due to total adult immobility.
- Sample storage conditions before analysis: Samples and back-up samples were stored refrigerated when not in use.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
On day 0 and on each renewal day (Monday, Wednesday, and Friday), a primary stock solution of 75.0 mg/L was prepared by adding 150 milligrams of the test substance and bringing this up to 2000 mL in test facility well water in a 4-L Erlenmeyer flask and stirring for approximately 1 hour for the day 0 stock solution and approximately 2 hours for all subsequent primary stock solutions prepared during the study. The stock solutions were slightly cloudy with surface film and undissolved test substance. Final nominal test concentrations of 3.15, 6.5, 12.5, 25, and 50 mg/L test substance (which correspond to nominal total solids concentrations of 0.63, 1.3, 2.5, 5.0, and 10 mg total solids/L) were prepared by diluting appropriate amounts of the primary stock solution with test facility well water. At test start and at each renewal, each test concentration and the dilution water control received 2.1 mL/L of algae (final algal concentration of approximately 62,500 cells/mL in test solutions) and 3 mL/L of yeast, cereal leaves and trout chow mixture (YCT). Final test solutions were stirred for approximately 7-10 minutes before use. At test start, the test facility well water and nominal 0.63 mg/L test solutions were cloudy and pale green in colour (due to the presence of algae) with visible particles of YCT. All other test solutions were cloudy and pale green with particles of YCT, a precipitate and surface film was also present.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea.
- Source: Reared at the performing lab.
- Age of parental stock (mean and range, SD): Neonates used in this test were less than 24 hours old and were collected from the 5th brood of 15-day old parent daphnids.
- Feeding during test: Yes.
- Food type and amount: Daphnids were fed on a daily basis with 3 mL/L of a yeast, cereal leaves and trout chow (YCT) mixture and the green alga, Pseudokirchneriella subcapitata, at a rate of approximately 62,500 cells/mL of culture media. The YCT mixture was standardized to 1700-2100 mg/L solids. The combination of YCT and alga is equivalent to approximately 0.1-0.2 mg total organic carbon per daphnid per day.
- Frequency: Daily
ACCLIMATION
- Daphnia magna were reared at the testing facility in 1000-mL glass beakers (8-10 per beaker) which contained 1000 mL of aerated, filtered testing facility well water and held at approximately 20.0°C.
- Type and amount of food: A combination of YCT (yeast, cereal leaves and trout chow mixture) and alga, equivalent to approximately 0.1-0.2 mg total organic carbon per daphnid per day. Algal feeding was 62,500 cells per mL of medium. The YCT mixture was standardized to 1700-2100 mg/L solids.
- Feeding frequency: Daily, excluding weekends and holidays.
- Health during acclimation (any mortality observed): No adult immobility was seen during the 48-hour pre-test period in the culture used to supply neonates for testing.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- Total alkalinity, EDTA hardness, and conductivity values for the dilution water control throughout the study ranged from 75-94 mg/L as CaCO3, 116-178 mg/L as CaCO3, and 201-332 µmhos/cm, respectively. Total alkalinity, EDTA hardness, and conductivity values for the highest test concentration with surviving organisms (nominal 50 mg/L test substance) throughout the study ranged from 83-100, 102-213, and 206-351, respectively.
- Test temperature:
- between 20.3 and 21.7°C
- pH:
- The pH ranged from 8.0 to 8.3 in new solutions and from 7.9 to 8.4 in old solutions but did not deviate by more than 0.5 units over a 48- or 72-hour period between renewals within the same replicate at all concentration levels.
- Dissolved oxygen:
- 8.3 mg/L or 91% of saturation (based on an air saturation value of 9.1 mg/L at 20°C
- Nominal and measured concentrations:
- Nominal: 0, 3.15, 6.5, 12.5, 25, and 50 mg/L
Corrected Nominal Solids: 0, 0.63, 1.3, 2.5, 5.0, and 10 mg total solids/L
Measured: 0, 0.439, 0.826, 1.66, 3.49, and 6.59 mg total solids/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (250 mL) containing 100 mL of test solution (3.3 cm depth)
- Renewal rate of test solution (frequency/flow rate): Test solutions were renewed every Monday, Wednesday, and Friday during the study.
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
OTHER TEST CONDITIONS
- Adjustment of pH: No data
- Photoperiod and light intensity: A photoperiod of 16 hours light (approximately 226-368 Lux) and 8 hours darkness was employed, which included approximately 30 minutes of transitional light (approximately 11-35 Lux) between the light and dark intervals.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Observations were made daily on the number of surviving adult daphnids, occurrence of sublethal effects, and production of live or immobile young. The criterion for immobility was the lack of response to application of a gentle stimulus. Immobile daphnids (adults and young) were discarded on renewal days. Live or immobile young were counted and removed on renewal days. Length and dry weight of surviving adult daphnids were determined at test end.
VEHICLE CONTROL PERFORMED: yes - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.59 other: mg total solids/L
- Conc. based on:
- other: Mean measured concentrations
- Basis for effect:
- other: all endpoints
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 6.59 other: mg total solids/L
- Conc. based on:
- other: Mean measured concentrations
- Basis for effect:
- other: all endpoints
- Duration:
- 21 d
- Dose descriptor:
- other: MATC (maximum-acceptable-toxicant concentration)
- Effect conc.:
- > 6.59 other: mg total solids/L
- Conc. based on:
- other: Mean measured concentrations
- Basis for effect:
- other: all endpoints
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 6.59 other: mg total solids/L
- Conc. based on:
- other: Mean measured concentrations
- Details on results:
- See Table 1.
There was a significant increasing trend in the total number of live young produced and in the total number of live young per surviving adult. Since an increase in young is not considered adverse, the NOEC for these two responses was determined to be the highest test concentration.
Any other information on results incl. tables
Table 1
|
Mean (Standard Deviation) |
|||||
Mean, Measured Concentrations (mg/L) |
Percent Adult Survivala |
First Reproduction Dayb |
Total Live Youngc |
Total Immobile Youngd |
Adult Length (mm)e |
Adult Dry Weight (mg)e |
Dilution Water Control |
80 |
8.5 (1.1) |
101.8 (22.0) |
0.0 (0.0) |
4.2 (0.2) |
0.6 (0.3) |
0.439 |
60 |
8.6 (2.1) |
82.0 (52.0) |
0.0 (0.0) |
4.1 (0.3) |
0.4 (0.3) |
0.826 |
60 |
8.2 (0.6) |
145.3 (13.5) |
0.0 (0.0) |
4.4 (0.2) |
0.5 (0.1) |
1.66 |
50 |
8.2 (1.1) |
150.2 (42.5) |
0.0 (0.0) |
4.3 (0.3) |
0.5 (0.1) |
3.49 |
90 |
7.8 (0.4) |
138.0 (24.6) |
0.0 (0.0) |
4.3 (0.3) |
0.5 (0.2) |
6.59 |
80 |
8.0 (0.7) |
147.1 (34.1) |
0.0 (0.0) |
4.2 (0.4) |
0.5 (0.1) |
a Percent of adult daphnids alive at the end of the test
b First day that reproduction was observed
c Sum of live young produced per surviving adult in 21 days
d Sum of immobile young produced per surviving adult in 21 days
e Per surviving adult
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability).
Based on mean measured concentrations and all endpoints at the end of the study:
21-day NOEC (no-observed-effect concentration) = 6.59 mg total solids/L
MATC (maximum-acceptable-toxicant concentration) >6.59 mg/L
LOEC (lowest-observed-effect concentration) >6.59 mg/L
EC50 for adult survival >6.59 mg/L - Executive summary:
In a 21-day chronic, static renewal study with Daphnia magna, a total of 10 replicates, each containing one, 24 hour-old neonate, were tested per concentration (10 neonates/concentration) and dilution water control. Nominal test substance concentrations were 0, 3.15, 6.5, 12.5, 25, and 50 mg/L. Corrected nominal solids concentrations were 0, 0.63, 1.3, 2.5, 5.0, and 10 mg total solids/L. Measured test substance concentrations were 0, 0.439, 0.826, 1.66, 3.49, and 6.59 mg total solids/L. The daphnids were fed Pseudokirchneriella subcapitata at a rate of approximately 62,500 cells/mL of test solution and 3 mL/L of a yeast, cereal leaves and trout chow mixture (YCT) on a daily basis. The combination of alga and YCT was equivalent to approximately 0.1-0.2 mg total organic carbon (TOC) per daphnid. Test solutions (old and new) were maintained between 20.3 and 21.7°C. Observations of immobilization, sublethal effects, and production of young were made daily. Length and dry weight of surviving adult daphnids were determined at test end. The 21-day NOEC (no-observed-effect concentration) was 6.59 mg total solids/L, based on mean measured test concentrations and all endpoints at the end of the study. The MATC (maximum-acceptable-toxicant concentration), and LOEC (lowest-observed-effect concentration) were >6.59 and >6.59 mg/L, respectively. The EC50 for adult survival was greater than >6.59 mg/L.
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