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EC number: 935-847-3 | CAS number: 1369773-39-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Sediment toxicity
Administrative data
Link to relevant study record(s)
- Endpoint:
- sediment toxicity: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jun - Oct 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 218 (Sediment-Water Chironomid Toxicity Test Using Spiked Sediment)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The distribution of 14C, deriving from 14C-labelled LBQ657 (900-04) and/or its transformation products, within the water, pore water and sediment was determined in the limit concentration. Furthermore, the LBQ657 (900-04) concentration in the spike solution used to prepare the sediment, including the radiochemical purity of 14C-labelled LBQ657 (900-04) was determined.
Frequency: Days 0 and 28. - Vehicle:
- yes
- Remarks:
- ethanol
- Details on sediment and application:
- The batch of LBQ657 (900-04) tested was a white powder. The purity of this batch is described in the Certificate of Analysis. The batch of [14C]LBQ657 was a solid with a radiochemical purity of 99% (as determined by the supplier), which was also verified at WIL Research Europe B.V.
Test concentrations were prepared by mixing spiked sand with sediment. For 1000 mg/kg d.w. a spike solution of 2.0 mg/ml in ethanol1, containing a mixture of 14C-labelled and non-labelled test substance was prepared. The ratio of 14C-labelled and non-labelled test substance was chosen so that the amount of radioactivity in the samples was sufficient to allow quantification of the radioactivity (approximately 0.1 MBq/vessel).
For 1000 mg/kg d.w. the amount of the spike solution
corresponded with approximately 60 mg/vessel. Per replicate (vessel) 30 g sand was spiked with 30 ml ethanol stock. After evaporation of the solvent the coated sand was mixed with sediment. Care was taken to ensure that LBQ657 (900-04) was thoroughly and evenly distributed within the sediment.
Test media were prepared bulk wise and subsequently distributed over replicates. The total amount of wet sediment was 85 g per test vessel, consisting of 30 g treated sand and 55 g OECD medium2. This corresponds theoretically with approximately 60 g dry sediment per vessel, based on the determined moisture (37%) the actual amount was 62 g dry sediment.
Preparation of the 1.0, 10 and 100 mg/kg d.w. test concentrations was comparable to the highest concentration, except that these concentrations were not performed with 14C-labelled LBQ657. Lower stock solutions were prepared by subsequent dilutions in ethanol. - Test organisms (species):
- Chironomus riparius
- Details on test organisms:
- temperature of ca. 20°C. The fertilized egg masses hatch 2 to 3 days after placing in water.
Thereafter the larvae undergo four stages. L2 larvae emigrate to the sediment. After the fourth stage, the larvae change into pupae. Immediately after the pupa moves to the surface of the water the midge emerges from the pupal skin. The emergence of male midges starts earlier than that of the females.
Females are a little larger and darker than males. Males have plumose antennae. Mating occurs in swarming. After 1-2 days the female midge lays an egg mass, 2 to 3 days later a second, but smaller, egg mass can be produced. Midges have a life span of four to eight days. - Study type:
- laboratory study
- Test type:
- static
- Water media type:
- freshwater
- Type of sediment:
- artificial sediment
- Limit test:
- yes
- Duration:
- 28 d
- Exposure phase:
- total exposure duration
- Remarks:
- 28 days (after introduction of the larvae)
- Post exposure observation period:
- Frequency: Days 0 and 28.
- Hardness:
- 196 – 232 mg CaCO3/l
- Test temperature:
- 20.0 ± 1.3°C
- pH:
- 7.7 – 8.4
- Dissolved oxygen:
- 7.0 – 8.9 mg/L
- Reference substance (positive control):
- no
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 280 mg/kg sediment dw
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- emergence rate
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 280 mg/kg sediment dw
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- development rate
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 280 mg/kg sediment dw
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- emergence rate
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 280 mg/kg sediment dw
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- development rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study with Chironomus riparius, no statistical significant reduction of emergence and no statistical significant effect on development rate were observed at 280 mg/kg d.w. (NOEC).
The 28-day EC50 for emergence and development rate was higher than 280 mg/kg d.w..
Reference
Description of key information
Under the conditions of the present study with Chironomus riparius, no statistical significant reduction of emergence and no statistical significant effect on development rate were observed at 280 mg/kg d.w. (NOEC).
The 28-day EC50 for emergence and development rate was higher than 280 mg/kg d.w..
Key value for chemical safety assessment
- EC10, LC10 or NOEC for freshwater sediment:
- 280 mg/kg sediment dw
Additional information
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