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EC number: 611-915-5 | CAS number: 59941-98-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- Adopted 22 July 2010
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test item was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 10 g/L. Therefore, the required amount of V123109 was added directly to the dark brown test-bottles containing 200 mL Milli-RO water (tap water purified by reverseosmosis; Millipore Corp., Bedford, Mass., USA). The test item – Milli-RO water mixtures were magnetically stirred for a period of 25 minutes. 16 mL synthetic medium to obtain 50 mL and 250 mL sludge was added to this solution, resulting in the required loading rates. Optimal contact between the test item and test organisms was ensured by applying continuous aeration and stirring.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: /
- Name and location of sewage treatment plant where inoculum was collected: Municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands
- Method of cultivation: /
- Preparation of inoculum for exposure: The sludge was coarsely sieved (1 mm) and allowed to settle. The supernatant was removed and ISO-medium was added. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.0 g/L of sludge, as used for the test). The pH was 7.7 on the day of testing. The batch of sludge was used one day after collection; therefore 50 mL of synthetic medium (=sewage feed) was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
- Pretreatment: /
- Initial biomass concentration: / - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- After the 3-hour contact time, the oxygen consumption was recorded for a period of approximately 10 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer.
- Test temperature:
- The temperature continuously measured in the temperature control vessels ranged between 20 and 22°C during the test
- pH:
- The pH in all test vessels, before addition of sludge was between 7.5 and 7.7. After the 3 hour exposure period the pH was between 7.2 and 8.2.
- Nominal and measured concentrations:
- loading rates of 10, 100 and 1000 mg/L were tested
- Details on test conditions:
- TEST SYSTEM
- Test vessel: All glass open bottles/vessels.
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: /
- Aeration: The aeration was adjusted in such a way that the dissolved oxygen concentration at the start was above 60-70% saturation (60% of air saturation is > 5 mg/L at 20°C) and to maintain the sludge flocs in suspension.
- No. of vessels per concentration (replicates): The highest loading rate was tested in triplicate, lower loading rates consisted of one replicate.
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): /
- No. of vessels per abiotic control (replicates): 1
- Sludge concentration (weight of dry solids per volume): suspended solids (3.0 g/L of sludge)
- Weight of dry solids per volume of reaction mixture per unit of time: /
- Nutrients provided for bacteria: /
- Biomass loading rate: /
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: /
- Particulate matter: /
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: /
- Light intensity: /
- Details on termination of incubation: /
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen consumption
TEST CONCENTRATIONS
- Spacing factor for test concentrations: /
- Justification for using fewer concentrations than requested by guideline: /
- Range finding study: Combined Limit/Range-Finding Test
- Test concentrations: 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: / - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- other: NOELR
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Remarks:
- for 3,5-dichlorophenol
- Effect conc.:
- 3.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The combined limit/range-finding test showed 11%, 8% and an average of 15% inhibition of the respiration rate at 10, 100 and 1000 mg/L, respectively. For V123109 no ELR50-value could be calculated because the test item proved to be non-toxic (ELR50 > 1000 mg/L)
No statistically significant inhibition of the respiration rate of the sludge was recorded at a concentration of 1000 mg V123109 per litre. Therefore, the NOELR was considered to be 1000 mg/L (see also Appendix 5).
There was no oxygen uptake from abiotic processes.
Furthermore, the mean control oxygen uptake rate was 29 mg O2/g.h, with a coefficient of variation of 13%, which was within the acceptable range. - Results with reference substance (positive control):
- Functioning of the test system was checked by testing 3,5-dichlorophenol which showed an acceptable EC50 (3.3 mg/L).
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this present test V123109 was not toxic to waste water bacteria (activated sludge) at a loading rate of 1000 mg/L (NOELR). The ELR50 was above 1000 mg/L.
- Executive summary:
The objective of the study was to evaluate V123109 for its ability to adversely affect aerobic microbial treatment plants and, if possible, to determine the ELR50and/or the no-observed effect loading rate (NOELR).
The influence of V123109 on the respiration rate of activated sludge was investigated after a contact time of 3 hours. The study procedures described in this report were based on the OECD guideline No. 209, 2010.
The batch of V123109 tested was a white crystalline powder with a purity of >95%. No correction was made for the purity/composition of the test item.
The test item was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 10 g/L. Therefore, weight amounts were added to the test-bottles containing Milli-RO water. The test item–Milli-RO water mixtures were magnetically stirred for a period of 25 minutes and subsequently, synthetic medium, sludge and Milli-RO water were added to obtain the required loading rates. Optimal contact between the test item and test medium was ensured by applying continuous aeration and stirring during the 3-hour exposure period. Thereafter, oxygen consumption was recorded for approximately 10 minutes.
In a combined limit/range-finding test loading rates of 10, 100 and 1000 mg/L were tested. The highest loading rate was tested in triplicate, lower loading rates consisted of one replicate. Furthermore, at 1000 mg/L an abiotic control (1 replicate) was tested. Responses were compared to the controls.
No statistically significant inhibition of the respiration rate of the sludge was recorded at a loading rate of 1000 mg V123109 per litre in the combined limit/range-finding test and subsequent testing was therefore not required.
The batch of activated sludge was tested for sensitivity with the reference item 3,5-dichlorophenol, and showed normal sensitivity. The study met the acceptability criteria prescribed by the study plan and was considered valid.
In conclusion, V123109 was not toxic to waste water (activated sludge) bacteria at a loading rate of 1000 mg/L (NOELR). The ELR50was above 1000 mg/L.
Reference
Experimental Conditions
The pH values in the individual vessels are presented inTable 3ofAppendix 2.The pH in all test vessels, before addition of sludge was between 7.5 and 7.7. After the 3 hour exposure period the pH was between 7.2 and 8.2.
The temperature continuously measured in the temperature control vessels ranged between 20 and 22°C during the test, and complied with the requirements as laid down in the study plan (20 ± 2°C.
Description of key information
Under the conditions of this present test V123109 was not toxic to waste water bacteria (activated sludge) at a loading rate of 1000 mg/L (NOELR). The ELR50 was above 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
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