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EC number: 604-638-6 | CAS number: 148520-82-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From July, 1978 to November, 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well-documented publication/study report which meets basic scientific principles.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 981
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- no
- Remarks:
- pre-dates GLPs
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Test material form:
- solid
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Not reported
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Not reported
Test organisms
- Test organisms (species):
- Lemna minor
- Details on test organisms:
- TEST ORGANISM
- Common name: Duckweed
- Strain: Not reported
- Source: The test species was originally collected near Boca Raton, Florida, and has been in continuous culture at the EPA Environmental Research Laboratory, Duluth, Minn., since 1971.
- Age of inoculum (at test initiation): Not reported
- Method of cultivation: Test species stocks were maintained in X0.01 Hutner's solution in a flow through system
ACCLIMATION
- Acclimation period: Not reported
- Culturing media and conditions (same as test or not): Not reported
- Any deformed or abnormal cells observed: Not reported
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Post exposure observation period:
- None
Test conditions
- Hardness:
- 120-130 (as CaCO3 ) mg/L
- Test temperature:
- 21-23 °C
- pH:
- 7.2-7.6
- Dissolved oxygen:
- 8.5 mg/L
- Nominal and measured concentrations:
- Nominal concentration: Not specified in the study report. All results are based on measured active ingredient. Details of analytical results are not provided in the study report. The test concentrations, based on measured active components, were: 0, 2.1, 3.8, 8, 17 and 34 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Incubation chamber used: Incubators were not used. However, the test chambers were placed in environmental control housing with a removable front cover for servicing.
- Test vessel: Pyrex glass test chambers
- Type: Open
- Material: All glass test chambers were separated into four individual compartments, each measuring 1.5 x 10.8 x 6.8 cm. Water volume in each compartment was regulated to approx. 400 mL
- Type of cover: Not reported
- Aeration: No (flow-through test)
- Agitation: No
- Type of flow-through: Proportional diluter
- Renewal rate of test solution: Water flow into the diluter was 1.5 L every 16 minutes, which was mixed with 6 mL nutrient stock to give the final concentration of 0.01X Hutner's solution in the test chambers. The flow rate through the individual test chambers was approximately 14 replacement volumes/day.
- Control end cells density: Not reported
- No. of colonies per vessel: Seven (two-frond, root-excised duckweed colonies)
- No. of fronds per colony: Two
- No. of vessels per concentration (replicates): Four
- No. of vessels per negative control (replicates): Four
GROWTH MEDIUM
- Standard medium used: No
- Detailed composition if non-standard medium was used: Nutrient stock solution used was X2.5 Hutner's solution which was mixed with dilution water to give the final concentration of X0.01 in the test chambers. Detailed composition of growth medium is not reported in the study.
SEDIMENT USED: Sediment was not used as the test species is a floating plant
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The dilution water was carbon- and reverse osmosis filtered well water, with added nutrients.
- Total organic carbon: Not reported
- Particulate matter: Not reported
- Metals (mg/L): Mercury = <0.005, Cadmium = <0.005, Zinc = <0.001, Lead = <0.005, Copper = <0.001, Iron = <0.05, Sodium = 11.6, Nickel = <0.033
- Pesticides (mg/L): Chlorinated insecticides= <0.00005, Organophosphate insecticides = <0.0005
- Nitrite: <0.05 mg/L
- Nitrate: <0.05 mg/L
- Chlorine: Not reported
- Alkalinity: Not reported
- Ca/mg ratio: Not reported
- Conductivity: Not reported
- Culture medium different from test medium: No
- Intervals of water quality measurement: Not reported
OTHER TEST CONDITIONS
- Sterile test conditions: Not reported
- Adjustment of pH: Not reported
- Photoperiod: Continuous illumination
- Light intensity and quality: 3875 lux (360 foot candles) fluorescent lights
EFFECT PARAMETERS MEASURED
- Determination of frond number: Number of fronds in each replicate chamber was recorded once every 24 h for 7 d. Every frond visibly projecting beyond the edge of the parent frond was counted manually.
- Determination of biomass: Dry weights were determined at the end of exposure period.
- Determination of frond area: Not determined
- Other: The root lengths were determined at the end of the exposure period. Where possible, 15 to 20 roots from each replicate weremeasured to the nearest 0.5 cm and the ten largest values were selected for statistical analysis. All plant material from each replicate was placed in a tared aluminum dish and dried at 103°C for 3 h. Since the growth rates stabilized by the Day 4, only frond count data from Days 4 through 7, and Day 7 root length and dry weight data were used for statistical analysis. From the frond count data, two other parameters growth rate and doubling time were computed or Day 4 through 7.
NEGATIVE CONTROL PERFORMED: Yes (received only carbon and reverse osmosis filtered well water)
RANGE-FINDING STUDY: No - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 3.6 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Remarks on result:
- other: 2.9-4.3
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 4.9 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: root length
- Remarks on result:
- other: 0-12.6
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 4.8 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 4.4-5.2
- Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 0.21 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- frond number
- Remarks on result:
- other: 0.16-0.38
- Duration:
- 7 d
- Dose descriptor:
- EC20
- Effect conc.:
- 0.53 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- frond number
- Remarks on result:
- other: 0.37-0.75
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 2.29 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- frond number
- Remarks on result:
- other: 1.9-2.7
- Details on results:
- - Any visual signs of phytotoxicity (abnormalities): Not reported
- Decrease in frond size: Not reported
- Necrosis / chlorosis: Not reported
- Sinking of fronds: Not reported
- Other: Frond count decreased dose dependently from Day 4 with increase in exposure concentration
- Any stimulation of growth found in any treatment: Not reported
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Not reported
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Endpoint values for frond count were derived using SAS v. 9.1.3. The frond count data were fit to an empirical non-linear model. The growth rate data were fit to empirical models derived directly from the frond count model. Root length and dry weight were fit to the same exponential model given by growth rate. See Appendix to study report for more details on statistical evaluations.
Any other information on results incl. tables
None
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 7d EC10 of C12 LABS Na (linear alkylbenzene sulfonate) to Lemna minor was 0.21 mg/L, based on frond count in a flow through test. The result is based on measured concentration.
- Executive summary:
A growth inhibition test to Lemna minor (duckweed), following OECD 221 guideline, was conducted on C11.8 LABS Na (linear alkylbenzene sulfonate). Endpoints included frond count, dry weight, growth rate, and root length after 7 d exposure period in a flow through study. The test concentrations were 0, 2.1, 3.8, 8, 17 and 34 mg/L (measured). There were 4 replicates at each test concentration.
The EC10 value, based on frond number, was 0.21 mg/L. The EC50 value, also based on frond number, was 2.30 mg/L.
This growth inhibition test is classified as acceptable, and satisfies the guideline requirements for the OECD 221.
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