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EC number: 614-396-3 | CAS number: 68298-12-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: screening test, other
- Remarks:
- short adhoc aerobic biodegradation experiment conducted for internal method development purposes
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Mar - Apr 2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Test substance was incubated in undiluted return activated sludge. At selected times aliquots were analyzed by HPLC/MS/MS.
- GLP compliance:
- no
- Remarks:
- screening level study conducted for internal method development purposes
Test material
- Reference substance name:
- 1,1,2,2,3,3,4,4,4-nonafluoro-N-methylbutane-1-sulfonamide
- EC Number:
- 614-396-3
- Cas Number:
- 68298-12-4
- Molecular formula:
- C5H4F9NO2S
- IUPAC Name:
- 1,1,2,2,3,3,4,4,4-nonafluoro-N-methylbutane-1-sulfonamide
Constituent 1
- Specific details on test material used for the study:
- - Purity: 97.25%
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Return activated sludge from River Mountains water treatment facility, Henderson, NV USA. Sludge was received on 26 Feb 2018 and used 19 Mar 2018.
- Storage length: 21 days
- Preparation of inoculum for exposure: none, sludge was used as received without dilution. - Duration of test (contact time):
- 18 d
Initial test substance concentration
- Initial conc.:
- 2.77 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Undiluted activated sludge. 20 mL of sludge was placed in a 125 mL flask, loosely capped to allow gas exchange.
- Additional substrate: no
- Solubilising agent: none
- Test temperature: 25 °
- pH: not reported
- pH adjusted: no
- Continuous darkness: not reported
TEST SYSTEM
- Culturing apparatus: Thermo Max-Q temperature controled shaker-incubator
- Number of culture flasks/concentration: one
- Method used to create aerobic conditions: shaking at 150 RPM
- Test performed in open system: yes
SAMPLING
- Sampling frequency: 1 mL samples taken on days 0, 1, 4, 8, 11, 15, and 18.
CONTROL AND BLANK SYSTEM
- Inoculum blank: None
- Abiotic sterile control: Autoclaved sludge, one replicate per substance
- Toxicity control: None
Reference substance
- Reference substance:
- other: 1,1,2,2,3,3,4,4,4-nonafluoro-N-(2-hydroxyethyl)-N-methylbutane-1-sulfonamide
- Remarks:
- substance has been previously examined and its biodegradation is well known.
Results and discussion
% Degradation
- Parameter:
- % degradation (test mat. analysis)
- Sampling time:
- 18 d
- Remarks on result:
- not determinable because of methodological limitations
- Remarks:
- mass balance was inadequate to compare loss of parent with degradation products
- Details on results:
- MeFBSA was incubated with viable activated sludge and autoclaved (sterile) activated sludge. Results are in Tables 1 and 2, respectively. Mass balance at the end of incubation was low in both active and sterile cultures (21 mole% and 4 mole%, respectively). Very little of the starting material could be recovered in both cases (2% and 4%, respectively). Demethylation to form 1,1,2,2,3,3,4,4,4-nonafluoro-1-Butanesulfonamide (major pathway) and deamidation to form perfluorobutanesulfinic acid (minor pathway) are the two degradation pathways, with the acid later oxidizing to form perfluorobutanesulfonic acid.
BOD5 / COD results
- Results with reference substance:
- As expected, the oxidation of the reference substance terminal group from hydroxyethyl to carboxymethyl was essentially complete by day 1.
Any other information on results incl. tables
Table 1, concentration of MeFBSA and potential metabolites (µM), viable sludge incubation
Day |
MeFBSA |
FBSA |
PFBSi |
PFBS |
PFBA |
Sum |
Mass balance |
0 |
6.2 |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
6.2 |
70% |
1 |
5.85 |
0.708 |
0.0198 |
<LOQ |
<LOQ |
6.57 |
74% |
4 |
4.79 |
1.09 |
0.0241 |
<LOQ |
<LOQ |
5.91 |
67% |
8 |
2.19 |
1.42 |
0.0378 |
<LOQ |
<LOQ |
3.65 |
41% |
11 |
1.25 |
1.51 |
0.0413 |
<LOQ |
<LOQ |
2.81 |
32% |
15 |
0.665 |
1.67 |
0.042 |
0.014 |
<LOQ |
2.4 |
27% |
18 |
0.179 |
1.61 |
0.0375 |
0.0186 |
<LOQ |
1.85 |
21% |
Mole % of dose on day 18 |
2% |
18% |
0.40% |
0.20% |
0% |
21% |
─ |
Table 2, concentration of MeFBSA and potential metabolites (µM), sterile sludge incubation
Day |
MeFBSA |
FBSA |
PFBSi |
PFBS |
PFBA |
Sum |
Mass balance |
0 |
5.75 |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
5.75 |
65% |
1 |
5.11 |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
5.11 |
58% |
4 |
3.09 |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
3.09 |
35% |
8 |
1.9 |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
1.9 |
22% |
11 |
1.17 |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
1.17 |
13% |
15 |
0.665 |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
0.665 |
8% |
18 |
0.371 |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
0.371 |
4% |
Mole % of dose on day 18 |
4% |
0% |
0% |
0% |
0% |
4% |
─ |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- other: incomplete mass balance, result cannot be interpreted
- Conclusions:
- MeFBSA is rapidly lost when incubated with viable or sterilized activated sludge.
- Executive summary:
MeFBSA biodegradation was assessed by incubating 18 days with undiluted activated sludge. The parent and several expected metabolites were analyzed by LC/MS immediately and on days 1, 4, 8, 11, 15, and 18. An autoclave sterilized sludge control was included. In both cases the parent was almost complete absent by day 18 (2% and 4% residual in viable and sterile incubations). In the viable flask, FBSA and PFBSi appeared at the same approximate times, with PFBS appearing later. However, these degradation products accounted for only 18%, 0.4%, and 0.2% of the starting material. No degradation products were detected in the sterile control.
The study does not establish half-life due to incomplete mass balance. However, as an exploratory study it demonstrates possible degradation pathways through specific chemical analysis. It is considered reliable with restrictions.
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