(2R)-2-(2-chlorophenyl)-2-hydroxyethanoic acid

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(2R)-2-(2-chlorophenyl)-2-hydroxyethanoic acid

EC Number:

444-040-0

EC Name:

(2R)-2-(2-chlorophenyl)-2-hydroxyethanoic acid

Cas Number:

52950-18-2

Molecular formula:

Hill formula: C8H7ClO3 CAS formula: C8H7CLO3

IUPAC Name:

(2R)-2-(2-chlorophenyl)-2-hydroxyacetic acid

Details on test material:

- Test substance: (R)-2-Chloromandelic acid
- purity: 99,7 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution, for the limit and range-finding tests, was prepared by dissolving the test item directly in LC reconstituted water
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: green algae
- Strain: CCAP 276/20.
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa, Institute of Freshwater Ecology, Far Sawrey, Ambleside, Cumbria, LA22 OLP, UK.
- Method of cultivation: the algae are cultured under sterile conditions and inaintained at exponential growth rate.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

24.1°C - 25.0°C

pH:

7.3-8.26

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass test vessels (250 mL Erlenmeyer flasks)
- Aeration: the cultures are constantly aerated with air filtered using a 0.22 porosity filter to maximize the CO2 availability (and thereby the cell growth) while maintaining the sterility.

- Initial cells density:
- Control end cells density: 1.0E+04 cells/mL

- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted-LC

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

RS-Freetext:
Effect values based on biomass:
-------------------------------
NOEbC (72 h): >/= 100 mg/L
EbC50 (72 h):   > 100 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes