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EC number: 284-515-8 | CAS number: 84929-31-7 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Citrus limonum, Rutaceae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Skin irritation: irritating
Eye irritation: not irritating
Key value for chemical safety assessment
Skin irritation / corrosion
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Eye irritation
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Additional information
Skin irritation potential of lemon oil was determined in an acute dermal toxicity limit test. Lemon oil was applied to abraded and unabraded skin of six male/female rabbits. A dose of 5 ml/kg of liquid Lemon oil was applied and occluded for 24 hours. After removal of the patches, local dermal reactions were scored according to the method of Draize. Toxic effects, dermal reactions, and mortality were recorded daily for 14 days. Erythema, edema, dicoloration, scaling and eschar formation were observed. As no detailed scoring data was available, it was not possible to classify lemon oil according to the EU classification criteria outlined in 67/548/EEC and 1272/2008. However, the available scores at 24 hrs indicate lemon oil should be considered irritating to the skin.
To determine if lemon oil produces any eye irritation, lemon oil was instilled into the eyes of three normal, healthy, albino rabbits. Irritation was scored according to the Draize scale for scoring ocular lesions every 24 hours for 4 days and then again on the 7thday.
Lemon oil did not produce any corneal opacity or iris congestion, but an intense conjunctival irritation did occur involving chemosis and discharge. On the 7thday of observation the treated eyes were normal. Based on the irritation scores and according to the EU classification criteria outlined in 67/548/EEC and 1272/2008, Lemon oil does not have to be classified as irritating to eye.
Effects on skin irritation/corrosion: irritating
Justification for classification or non-classification
Based on the available information, lemon oil has been shown to have irritating properties when applied to the skin. Although the available information cannot be used for classification according to the criteria outlined in Annex VI of 67/548/EEC and Annex I of 1272/2008/EC (CLP/EU-GHS), lemon oil should be considered to have skin irritating properties.
The results of the primary eye irritation test indicate that the substance lemon oil does not need to be classified as eye irritant according to the criteria outlined in Annex VI of 67/548/EEC and Annex I of 1272/2008/EC (CLP/EU-GHS).
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