Lanolin, acetate

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Remarks:

HPLC screening method to determine the Adsorption Coefficient

Type of information:

experimental study

Adequacy of study:

key study

Study period:

03 March 2016 to 05 March 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes

Type of method:

HPLC estimation method

Remarks:

A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.

Test material

Test material information

Constituent 1

Reference substance name:

Lanolin, acetate

EC Number:

262-979-2

EC Name:

Lanolin, acetate

Cas Number:

61788-48-5

Molecular formula:

Variable

IUPAC Name:

Lanolin, Acetates

Test material form:

solid

Radiolabelling:

no

Study design

Test temperature:

30 °C

HPLC method

Details on study design: HPLC method:

EQUIPMENT
- Apparatus: Agilent Technologies 1200, incorporating workstation and autosampler
- Type: A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.
- Type, material and dimension of analytical (guard) column: Waters XSelect HSS CN 5μm (150 x 4.6 mm id)
- Detection system: Agilent variable wavelength detector (VWD) and Polymer Laboratories evaporative light scattering detector 2100 (ELSD)

MOBILE PHASES
- Type: methanol:water (55:45 v/v)
- Experiments with additives carried out on separate columns: no, the sample, dead time and reference standard solutions were injected in duplicate.
- pH: 6.6
- Solutes for dissolving test and reference substances: in methanol

DETERMINATION OF DEAD TIME
- Method: The dead time was determined by measuring the retention time of formamide (purity of 99.94%) at 580 mg/L solution in methanol.

REFERENCE SUBSTANCES (prepared in methanol)
- Identity:
Acetanilide (99+ purity) conc = 104 mg/L;
Atrazine (99.1 purity) conc = 109 mg/L;
Isoproturon (>98.0 purity) conc = 109 mg/L;
Triadimenol (98.0 purity) conc = 108 mg/L;
Linuron (99.7 purity) conc = 111 mg/L;
Naphthalene (99+ purity) conc = 102 mg/L;
Endosulfan-diol (99.9 purity) conc = 110 mg/L;
Fenthion (97.9 purity) conc = 113 mg/L;
α-Endosulfan (99.6 purity) conc = 104 mg/L;
Phenanthrene (>=99.5 purity) conc = 108 mg/L;
Diclofop-methyl (99.1 purity) conc = 108 mg/L;
DDT (98.7 purity) conc = 112 mg/L.

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 0.0255 g was diluted to 10 mL
- Quantity of reference substances: (See reference substance quantities ablove)
- Intervals of calibration: (See table below)

REPETITIONS
- Number of determinations: 3

EVALUATION
- Calculation of capacity factors k': (See table below)
- Calculation of retention times: (See table below)
- Determination of the log Koc value: (See table below)

Batch equilibrium or other method

Analytical monitoring:

no

Results and discussion

Adsorption coefficient

Adsorption coefficient 1

Key result

Sample No.:

#1

Type:

log Koc

Value:

> 5.63 dimensionless

pH:

6.6

Temp.:

30 °C

Adsorption coefficient 2

Key result

Sample No.:

#1

Type:

other: Adsorption coeficient range

Value:

427 000 dimensionless

pH:

6.6

Temp.:

30 °C

Results: HPLC method

Details on results (HPLC method):

see table below

Any other information on results incl. tables

The mobile phase pH was unadjusted as the majority of the components of the test item (a complex mixture) had no dissociation constant within the environmentally relevant pH range so they were tested in their non-ionized form.

Adsorption Coefficient: The retention times, capacity factor and log10 Koc value determined for the sample are shown in the following table:

Peak	Injection	Retention Time (mins)	Capacity Factor (k')	Log10 k'	Log10Koc	Mean log10Koc
1	1	36.620	>10.7	>1.03	>5.63	>5.63
	2	36.620	>10.7	>1.03	>5.63

Overall log10 Koc range: >5.63

Adsorption coefficient range: >4.27 x 10^5

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient of the test item has been determined to be greater than 4.27 x 10^5, log10 Koc >5.63.

Executive summary:

Adsorption Coefficient was >4.27 x 105, log10 Koc >5.63 using the HPLC screening method, designed to be compatible with Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001.