Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 261-699-8 | CAS number: 59323-76-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study performed under GLP. All relevant validity criteria were met.
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- inspected February 2000; signature: April 2000
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): mixed population of sewage sludge micro-organisms was obtained from the final effluent stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK, which treats predominantly domestic sewage.
- Storage conditions: Room temperature (at 21°C).
- Storage length: Used on day of collection.
- Preparation of inoculum for exposure: The sample of effluent was filtered through coarse filter paper (first approximate 200 ml discarded) and the filtrate maintained on continuous aeration.
- Initial cell/biomass concentration: Not reported.
- Water filtered: yes (water used to prepare culture medium).
- Type and size of filter used, if any: Aerated reverse osmosis purified and deionised water (Elga Optima 15+). The 'dilution water' was kept in a temperature controlled room, at approximately 21 °C, under gentle aeration overnight prior to use. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Solution a: KH2PO4 8.50 g/L; K2HPO4 21.75 g/L; Na2HPO4.12H2O 33.40 g/L ; NH4Cl 0.50 g/L ; pH = 7.4 ; Solution c: MgSO4.7H2O 22.50 g/L ; Solution b: CaCl2 22.75 g/L ; Solution d: FeCl3.6H2O 0.25 g/L. 1 litre medium contains: 1 ml of solution (A to (D) and made up to 1 litre aerated reverse osmosis purified and deionised water (Elga Optima 15+). The concentration of dissolved oxygen was measured for control purposes. Test item concentration: In duplicate at 2 mg/l. Procedure control: Reference substance sodium benzoate, concentration 3 mg/L; Toxicity control: concentration 2 mg/l sodium benzoate plus 1.5 mg/l test item.
- Solubilising agent (type and concentration if used): None
- Test temperature: The BOD bottles were incubated in a temperature controlled water bath at 20°C.
- pH: Not reported.
- pH adjusted: No.
- Continuous darkness: Yes.
TEST SYSTEM
- Culturing apparatus: 250-300 ml BOD bottles with glass stoppers
- Number of culture flasks/concentration: In duplicate. The test media were transferred by siphon to BOD bottles, which were firmly stoppered to
exclude all air bubbles. Sufficient bottles were prepared to allow a single oxygen determination per bottle with duplicate bottles for each test medium at each sampling occasion.
- Measuring equipment: Yellow Springs oxygen meter and BOD Probe.
- Test performed in closed vessels due to significant volatility of test substance: No.
- Test performed in open system: No.
- Details of trap for CO2 and volatile organics if used: Not applicable.
SAMPLING
- Sampling frequency: In duplicate; on Days 0, 3, 6, 9, 12, 14, 18, 21,24 and 28
- Sampling method: See measuring equipment and sampling frequency above.
- Sterility check if applicable: Not reported.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes.
- Abiotic sterile control: No.
- Toxicity control: Yes.
- Other: Positive control - using reference item (Sodium Benzoate) only. - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 7
- Sampling time:
- 28 d
- Remarks on result:
- other: mean % degradation; 2 mg/l test item concentration
- Results with reference substance:
- The results with the reference substance were valid.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: Not readily biodegradable
- Conclusions:
- The test item attained 7% mean degradation after 28 day in duplicate. The test item cannot be considered to be readily biodegradable.
- Executive summary:
The ready biodegradability test was carried out according to OECD 301D (Ready Biodegradability: Closed Bottle Test), EU Method C4-E and EPA OPPTS 835.3110 guidelines under GLP. The test item, at a concentration of 2.0 mg/l, was exposed to sewage treatment micro-organisms with culture medium in sealed culture vessels in the dark at 20°C for 28 days. The degradation of the test item was assessed by the determination of the amount of oxygen consumed. Control solutions with inoculum and the standard item, sodium benzoate, together with a toxicity control were used for validation purposes. Oxygen concentration measurements were in duplicate; on Days 0, 3, 6, 9, 12, 14, 18, 21,24 and 28. In the toxicity control, the test substance was found not to inhibit microbial activity. All of the validity criteria for the test were met. Under the conditions of this study, the test item attained 7% degradation after 28 days and therefore cannot be considered as readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 2003
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Guideline study performed under GLP. All relevant validity criteria were met. The source and target substances must have an ether group at the 1-position and a thioether at the 3-position of the cyclic six membered ring and thereby fulfil the 1,3-oxathiane definition. Single alkyl group (preferably smaller than an n-butyl group; should be present at the 2-position. Either a single or dual alkyl groups (preferable smaller than or equal to an n-butyl group) should be present at the 4-position. The target and source share common structural elements in the same relative positions. The source and target have very similar physico-chemical properties and therefore demonstrative chemical similarity.
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Further information is included in attachment to IUCLID section 13.
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
The read-across is based on the hypothesis that the source and target substances have common structural features in the same relative positions. The source and target have similar physico-chemical, toxicological properties and because of common metabolism they share common or have similar breakdown products and therefore potential mechanisms of action. Further information is included in attachment to IUCLID section 13.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
The source and target chemicals have comparable chemical similarity. Further information is included in attachment to IUCLID section 13
3. ANALOGUE APPROACH JUSTIFICATION
The source substance is a chemically similar substance with common metabolism and common or similar degradants of the target substance. Further information is included in attachment to IUCLID section 13
4. DATA MATRIX
Further information is included in attachment to IUCLID section 13. - Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- inspected February 2000; signature: April 2000
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): mixed population of sewage sludge micro-organisms was obtained from the final effluent stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK, which treats predominantly domestic sewage.
- Storage conditions: Room temperature (at 21°C).
- Storage length: Used on day of collection.
- Preparation of inoculum for exposure: The sample of effluent was filtered through coarse filter paper (first approximate 200 ml discarded) and the filtrate maintained on continuous aeration.
- Initial cell/biomass concentration: Not reported.
- Water filtered: yes (water used to prepare culture medium).
- Type and size of filter used, if any: Aerated reverse osmosis purified and deionised water (Elga Optima 15+). The 'dilution water' was kept in a temperature controlled room, at approximately 21 °C, under gentle aeration overnight prior to use. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Solution a: KH2PO4 8.50 g/L; K2HPO4 21.75 g/L; Na2HPO4.12H2O 33.40 g/L ; NH4Cl 0.50 g/L ; pH = 7.4 ; Solution c: MgSO4.7H2O 22.50 g/L ; Solution b: CaCl2 22.75 g/L ; Solution d: FeCl3.6H2O 0.25 g/L. 1 litre medium contains: 1 ml of solution (A to (D) and made up to 1 litre aerated reverse osmosis purified and deionised water (Elga Optima 15+). The concentration of dissolved oxygen was measured for control purposes. Test item concentration: In duplicate at 2 mg/l. Procedure control: Reference substance sodium benzoate, concentration 3 mg/L; Toxicity control: concentration 2 mg/l sodium benzoate plus 1.5 mg/l test item.
- Solubilising agent (type and concentration if used): None
- Test temperature: The BOD bottles were incubated in a temperature controlled water bath at 20°C.
- pH: Not reported.
- pH adjusted: No.
- Continuous darkness: Yes.
TEST SYSTEM
- Culturing apparatus: 250-300 ml BOD bottles with glass stoppers
- Number of culture flasks/concentration: In duplicate. The test media were transferred by siphon to BOD bottles, which were firmly stoppered to
exclude all air bubbles. Sufficient bottles were prepared to allow a single oxygen determination per bottle with duplicate bottles for each test medium at each sampling occasion.
- Measuring equipment: Yellow Springs oxygen meter and BOD Probe.
- Test performed in closed vessels due to significant volatility of test substance: No.
- Test performed in open system: No.
- Details of trap for CO2 and volatile organics if used: Not applicable.
SAMPLING
- Sampling frequency: In duplicate; on Days 0, 3, 6, 9, 12, 14, 18, 21,24 and 28
- Sampling method: See measuring equipment and sampling frequency above.
- Sterility check if applicable: Not reported.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes.
- Abiotic sterile control: No.
- Toxicity control: Yes.
- Other: Positive control - using reference item (Sodium Benzoate) only. - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 7
- Sampling time:
- 28 d
- Remarks on result:
- other: mean % degradation; 2 mg/l test item concentration
- Results with reference substance:
- The results with the reference substance were valid.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: Not readily biodegradable
- Conclusions:
- The target substance: is expected to not be ready biodegradable.
- Executive summary:
The ready biodegradability test was carried out on a source substance according to OECD 301D (Ready Biodegradability: Closed Bottle Test), EU Method C4-E and EPA OPPTS 835.3110 guidelines under GLP. The test item, at a concentration of 2.0 mg/l, was exposed to sewage treatment micro-organisms with culture medium in sealed culture vessels in the dark at 20°C for 28 days. The degradation of the test item was assessed by the determination of the amount of oxygen consumed. Control solutions with inoculum and the standard item, sodium benzoate, together with a toxicity control were used for validation purposes. Oxygen concentration measurements were in duplicate; on Days 0, 3, 6, 9, 12, 14, 18, 21,24 and 28. In the toxicity control, the test substance was found not to inhibit microbial activity. All of the validity criteria for the test were met. Under the conditions of this study, the test item attained 7% degradation after 28 days and therefore cannot be considered as readily biodegradable.
The target substance: is not expected be ready biodegradable in accordance with Regulation (EC) 1272/2008.
Referenceopen allclose all
Table 1. Dissolved oxygen measurements
Test Series |
Day |
Dissolved Oxygen (mg O2/l) |
|||||||||
0 |
3 |
6 |
9 |
12 |
14 |
18 |
21 |
24 |
28 |
||
a) Culture Medium with Inoculum
|
R1 |
8.90 |
8.70 |
8.60 |
8.50 |
8.35 |
8.40 |
8.20 |
8.10 |
7.90 |
7.90 |
R2 |
8.90 |
8.80 |
8.60 |
8.50 |
8.40 |
8.30 |
8.10 |
7.95 |
7.90 |
7.80 |
|
b) Sodium Benzoate (3.0 mg/l) with Inoculum |
R1 |
8.90 |
6.20 |
5.80 |
5.40 |
4.90 |
4.90 |
4.70 |
4.40 |
4.40 |
4.20 |
R2 |
8.90 |
5.80 |
5.50 |
5.30 |
4.80 |
4.80 |
4.85 |
4.40 |
4.25 |
4.30 |
|
c) Test Material (2.0 mg/l) with Inoculum |
R1 |
8.90 |
8.60 |
8.50 |
8.20 |
8.25 |
8.20 |
7.90 |
7.60 |
7.70 |
7.50 |
R2 |
8.90 |
8.60 |
8.50 |
8.30 |
8.20 |
8.20 |
7.90 |
7.65 |
7.60 |
7.50 |
|
d) Test Material (2.0 mg/l) plus Sodium Benzoate (1.5mg/l) with Inoculum |
R1 |
8.90 |
7.30 |
7.10 |
7.10 |
6.65 |
6.50 |
6.20 |
5.90 |
5.90 |
5.70 |
R2 |
8.90 |
7.30 |
7.10 |
7.00 |
6.60 |
6.40 |
6.30 |
6.00 |
5.80 |
5.80 |
Table 2. Oxygen Depletion and Mean Percentage Biodegradation Values
Test Series |
|
|
Day |
||||||||
3 |
6 |
9 |
12 |
14 |
18 |
21 |
24 |
28 |
|||
a) Culture Medium with Inoculum |
Mean O2 Depletion (mg O2/l) |
0.150 |
0.300 |
0.400 |
0.525 |
0.550 |
0.750 |
0.875 |
1.000 |
1.050 |
|
b) Sodium Benzoate (3.0 mg/l) with Inoculum |
O2 Depletion (mg O2/l) |
R1 |
2.550 |
2.800 |
3.100 |
3.475 |
3.450 |
3.450 |
3.625 |
3.500 |
3.650 |
R2 |
2.950 |
3.100 |
3.200 |
3.575 |
3.550 |
3.300 |
3.625 |
3.650 |
3.550 |
||
% Degradation (mean) |
55 |
59 |
63 |
70 |
70 |
68 |
72 |
72 |
72 |
||
c) Test Material (2.0 mg/l) with Inoculum |
O2 Depletion (mg O2/l) |
R1 |
0.150 |
0.100 |
0.300 |
0.125 |
0.150 |
0.250 |
0.425 |
0.200 |
0.350 |
R2 |
0.150 |
0.100 |
0.200 |
0.175 |
0.150 |
0.250 |
0.375 |
0.300 |
0.300 |
||
% Degradation (mean) |
3 |
2 |
5 |
3 |
3 |
5 |
8 |
5 |
7 |
||
d) Test Material (2.0 mg/l) plus Sodium Benzoate (1.5mg/l) with Inoculum |
O2 Depletion (mg O2/l) |
R1 |
1.450 |
1.500 |
1.400 |
1.725 |
1.850 |
1.950 |
2.125 |
2.000 |
2.150 |
R2 |
1.450 |
1.500 |
1.500 |
1.775 |
1.950 |
1.850 |
2.025 |
2.100 |
2.050 |
||
% Degradation (mean) |
19 |
19 |
19 |
23 |
25 |
25 |
27 |
27 |
28 |
Other information:
- The oxygen depletion of the inoculated control did not exceed 1.5 mg O2/l after 28 days, the residual oxygen concentration in the test bottles remained at 4.20 mg O2/l or greater in all test vessels.
- The difference between the extremes of replicate oxygen depletion values at the end of the test was less than 20% in all vessels thereby satisfying the validation criteria.
- On Day 12 the toxicity control had attained 23% degradation. Dissolved oxygen concentration measurements were taken on Day 14 in order to confirm that the test material had satisfied the validation criterion given in the test guidelines whereby > or = 25% degradation must be obtained by the toxicity control by Day 14 for the test material to be considered as non-inhibitory. After 28 days the toxicity control had attained 28% degradation.
- The positive control or standard material sodium benzoate, attained 72% degradation after 28 days thereby confirming the suitability of the test method and culture conditions.
Table 1. Dissolved oxygen measurements
Test Series |
Day |
Dissolved Oxygen (mg O2/l) |
|||||||||
0 |
3 |
6 |
9 |
12 |
14 |
18 |
21 |
24 |
28 |
||
a) Culture Medium with Inoculum
|
R1 |
8.90 |
8.70 |
8.60 |
8.50 |
8.35 |
8.40 |
8.20 |
8.10 |
7.90 |
7.90 |
R2 |
8.90 |
8.80 |
8.60 |
8.50 |
8.40 |
8.30 |
8.10 |
7.95 |
7.90 |
7.80 |
|
b) Sodium Benzoate (3.0 mg/l) with Inoculum |
R1 |
8.90 |
6.20 |
5.80 |
5.40 |
4.90 |
4.90 |
4.70 |
4.40 |
4.40 |
4.20 |
R2 |
8.90 |
5.80 |
5.50 |
5.30 |
4.80 |
4.80 |
4.85 |
4.40 |
4.25 |
4.30 |
|
c) Test Material (2.0 mg/l) with Inoculum |
R1 |
8.90 |
8.60 |
8.50 |
8.20 |
8.25 |
8.20 |
7.90 |
7.60 |
7.70 |
7.50 |
R2 |
8.90 |
8.60 |
8.50 |
8.30 |
8.20 |
8.20 |
7.90 |
7.65 |
7.60 |
7.50 |
|
d) Test Material (2.0 mg/l) plus Sodium Benzoate (1.5mg/l) with Inoculum |
R1 |
8.90 |
7.30 |
7.10 |
7.10 |
6.65 |
6.50 |
6.20 |
5.90 |
5.90 |
5.70 |
R2 |
8.90 |
7.30 |
7.10 |
7.00 |
6.60 |
6.40 |
6.30 |
6.00 |
5.80 |
5.80 |
Table 2. Oxygen Depletion and Mean Percentage Biodegradation Values
Test Series |
|
|
Day |
||||||||
3 |
6 |
9 |
12 |
14 |
18 |
21 |
24 |
28 |
|||
a) Culture Medium with Inoculum |
Mean O2 Depletion (mg O2/l) |
0.150 |
0.300 |
0.400 |
0.525 |
0.550 |
0.750 |
0.875 |
1.000 |
1.050 |
|
b) Sodium Benzoate (3.0 mg/l) with Inoculum |
O2 Depletion (mg O2/l) |
R1 |
2.550 |
2.800 |
3.100 |
3.475 |
3.450 |
3.450 |
3.625 |
3.500 |
3.650 |
R2 |
2.950 |
3.100 |
3.200 |
3.575 |
3.550 |
3.300 |
3.625 |
3.650 |
3.550 |
||
% Degradation (mean) |
55 |
59 |
63 |
70 |
70 |
68 |
72 |
72 |
72 |
||
c) Test Material (2.0 mg/l) with Inoculum |
O2 Depletion (mg O2/l) |
R1 |
0.150 |
0.100 |
0.300 |
0.125 |
0.150 |
0.250 |
0.425 |
0.200 |
0.350 |
R2 |
0.150 |
0.100 |
0.200 |
0.175 |
0.150 |
0.250 |
0.375 |
0.300 |
0.300 |
||
% Degradation (mean) |
3 |
2 |
5 |
3 |
3 |
5 |
8 |
5 |
7 |
||
d) Test Material (2.0 mg/l) plus Sodium Benzoate (1.5mg/l) with Inoculum |
O2 Depletion (mg O2/l) |
R1 |
1.450 |
1.500 |
1.400 |
1.725 |
1.850 |
1.950 |
2.125 |
2.000 |
2.150 |
R2 |
1.450 |
1.500 |
1.500 |
1.775 |
1.950 |
1.850 |
2.025 |
2.100 |
2.050 |
||
% Degradation (mean) |
19 |
19 |
19 |
23 |
25 |
25 |
27 |
27 |
28 |
Other information:
- The oxygen depletion of the inoculated control did not exceed 1.5 mg O2/l after 28 days, the residual oxygen concentration in the test bottles remained at 4.20 mg O2/l or greater in all test vessels.
- The difference between the extremes of replicate oxygen depletion values at the end of the test was less than 20% in all vessels thereby satisfying the validation criteria.
- On Day 12 the toxicity control had attained 23% degradation. Dissolved oxygen concentration measurements were taken on Day 14 in order to confirm that the test material had satisfied the validation criterion given in the test guidelines whereby > or = 25% degradation must be obtained by the toxicity control by Day 14 for the test material to be considered as non-inhibitory. After 28 days the toxicity control had attained 28% degradation.
- The positive control or standard material sodium benzoate, attained 72% degradation after 28 days thereby confirming the suitability of the test method and culture conditions.
Description of key information
Ready Biodegradability (Read-Across: 2-Ethyl-4,4-dimethyl-1,3-oxathiane): not readily biodegradable, 7 % mean degradation (28-d) , OECD TG 301D, 2003
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Key Study: OECD TG 301D, 2003 : Read-Across SOURCE (2-Ethyl-4,4-dimethyl-1,3-oxathiane) : The ready biodegradability test was carried out according to OECD 301D (Ready Biodegradability: Closed Bottle Test), EU Method C4-E and EPA OPPTS 835.3110 guidelines under GLP. The test item, at a concentration of 2.0 mg/l, was exposed to sewage treatment micro-organisms with culture medium in sealed culture vessels in the dark at 20°C for 28 days. The degradation of the test item was assessed by the determination of the amount of oxygen consumed. Control solutions with inoculum and the standard item, sodium benzoate, together with a toxicity control were used for validation purposes. Oxygen concentration measurements were in duplicate; on Days 0, 3, 6, 9, 12, 14, 18, 21,24 and 28. In the toxicity control, the test substance was found not to inhibit microbial activity. All of the validity criteria for the test were met. Under the conditions of this study, the test item attained 7% degradation after 28 days and therefore cannot be considered as readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)