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Diss Factsheets
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EC number: 258-081-5 | CAS number: 52663-87-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, GLP, quality criteria met. limit test, lack of details on substance identity. no analytical measurement of test concentration.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not relevant - Analytical monitoring:
- no
- Details on sampling:
- Not relevant
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was dispersed directly in test water to give a stock solution of 2000 mg a.i./l. The desired exposure level was obtained by adjusting 1 litre of this stock with test water to a final volume of 20 litres.
- Controls: Yes, blanks - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Westacre trout farm, Norfolk, UK
- Age at study initiation (mean and range, SD):
- Length at study termination (controls): 5.3 +/- 0.9 cm
- Weight at study termination (controls): 2.33 +/- 0.96 g
- Feeding during test: no feeding during the test, starting 24h prior to test
ACCLIMATION
- Acclimation period: 3 months
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: commercial trout pellets
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no mortality in the week prior to the start of the experiment - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not relevant
- Hardness:
- 154 mg/l CaCO3
- Test temperature:
- 14 - 15ºC
- pH:
- 7.7 - 8.0
- Dissolved oxygen:
- 8.98 - 10.00 mg/l
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Nominal: 100 mg a.i./l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: aquarium
- Material, size, headspace, fill volume: glass, containing 20 litres of test medium
- Aeration: None
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1- Biomass loading rate: 1.16 g/litre
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: laboratory tap water filtered, dechlorinated and passed through an Elga water purification system. The water passed in sequence through a high-pressure sand filter to remove colloids and particulate matter and a high grade activated carbon filter to remove chlorine and any organic contaminants. A proportion of the supply the passes through a water softener before final reverse osmosis treatment to produce a highly purified water supply. The two grades of dechlorinated water are the remixed in the ratio of approximately 1:1 to give a supply with the desired water hardness.
- Conductivity: 1000 uS/cm
- Chlorine: below limit of detection
- Culture medium different from test medium: No
- Intervals of water quality measurement: 24 hours
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16/8 hour light/dark photoperiod
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality and sublethal effects (observed daily) - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: 0%
- Results with reference substance (positive control):
- Not relevant
- Reported statistics and error estimates:
- No data
- Sublethal observations / clinical signs:
For detailed results, see attached file "Results.docx".
- Validity criteria fulfilled:
- yes
- Remarks:
- Mortality in controls 0%, abiotic conditions within limits
- Conclusions:
- The acute toxicity (96h-LC50) of test item towards Oncorhynchys mykiss is > 100 mg a.i./l.
- Executive summary:
The acute toxicity of the test item towards Oncorhynchys mykiss was investigated according to OECD Guideline 203 under GLP. Fish were exposed to a concentration 100 mg a.i./ for 96 hours. The 96h-NOEC and 96h-LC50 were found to be > 100 mg a.i./l.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Justification for type of information:
- See read-across justification attached in Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
Referenceopen allclose all
Description of key information
An analogue test material gave LC50 (96 h) > 100 mg a.i./L based on mortality of Oncorhynchys mykiss (OECD 203).
Key value for chemical safety assessment
Additional information
The acute toxicity of an analogue test item towards Oncorhynchys mykiss was investigated according to OECD Guideline 203 under GLP. Fish were exposed to a concentration 100 mg a.i./ for 96 hours. The 96h-NOEC and 96h-LC50 were found to be > 100 mg a.i./L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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