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EC number: 242-644-7 | CAS number: 18880-36-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-04-12 - 2016-04-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD 202 guideline study under GLP on the registered substance itself without deviations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- OECD Guideline for Testing of Chemicals No. 202, adopted 13. Apr. 2004: ”Daphnia sp., Acute Immobilisation Test“
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- Council Regulation (EC) No. 440/2008, Method C.2. “Daphnia sp. Acute Immobilisation Test”, adopted 30. May 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Kaiser-Friedrich-Straße 7, 55116 Mainz, Germany
Test material
- Reference substance name:
- Sodium 3-[[(dimethylamino)thioxomethyl]thio]propanesulphonate
- EC Number:
- 242-644-7
- EC Name:
- Sodium 3-[[(dimethylamino)thioxomethyl]thio]propanesulphonate
- Cas Number:
- 18880-36-9
- Molecular formula:
- C6H13NO3S3.Na
- IUPAC Name:
- sodium 3-[(dimethylcarbamothioyl)sulfanyl]propane-1-sulfonate
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Name of test material (as cited in study report): sodium 3-[[(dimethylamino)thioxomethyl]thio]propanesulphonate, DPS
- Substance type: pure substance
- Storage condition of test material: The test item was stored in a tightly closed vessel at 15.2- 20.9 °C under dry conditions in the dark.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 10, 18, 32, 56, 100 mg/L
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution containing 100.8 mg/L test item in dilution water was prepared. The solutions of the amount needed were prepared by dilution of this stock solution with dilution water.
- Controls: Blank control were 4 vessels, each containing 20 mL dilution water and 5 Daphnia, Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none stated
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain: Berlin
- Source: Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical.
- Age at study initiation (mean and range, SD):
- Method of breeding: The husbandry is performed similar to the method described in EN ISO 6341, following SOP 115 002 01 („Zucht und Hälterung von Daphnia magna STRAUS“), Version 12.
Vessels: preserving glasses, nominal volume 2 L
Medium: M4-Medium (recipe of ELENDT)
Food: unicellular green algae (Desmodesmus subspicatus)
Medium renewal: twice a week
- Feeding during test: none
ACCLIMATION
- Acclimation period: none, in-house breeding
- Acclimation conditions (same as test or not): Not the same, but switching from M4-medium (husbandry) to Dilution water (test) has been shown not to cause any detrimental effects for test Daphnia.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 2.502 mmol/L; 250 mg CaCO3/L
- Test temperature:
- 18.7 – 21.2 °C
- pH:
- 7.6 (0h) - 7.7 (48h)
- Dissolved oxygen:
- 8.5 - 9.3 mg/L
- Nominal and measured concentrations:
- 0, 10, 18, 32, 56, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers, nominal volume 50 mL, tall shape
- Material, size, headspace, fill volume: 30 ml headspace
- Aeration: After preparation, the dilution water was aerated.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Adjustment of pH: After preparation, the dilution water was aerated, the pH measured and if necessary adjusted to 8.4.
- Photoperiod / Light intensity: diffuse lighting
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : After 24 and 48 hours, the immobilised Daphnia were counted. The pH, the concentration of dissolved oxygen and the content of the test item in the test vessels were measured at the beginning and at the end of the test.
TEST CONCENTRATIONS
- Test concentrations: 0, 10, 18, 32, 56, 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: none noted
- Mortality of control: 0%
- Other adverse effects control: none noted
- Abnormal responses: none noted
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none noted
- Effect concentrations exceeding solubility of substance in test medium: none - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 24h-EC50 of K2Cr2O7 was determined as 1.6 mg/L in a separate GLP study, lying within the demanded range of 0.6 – 1.7 mg/L stated in the guideline
- Limit test: no
- Dose-response test: yes - Reported statistics and error estimates:
- 95% confidence interval could not be determined as no immobilisation occurred.
Any other information on results incl. tables
Findings
Immobility
In the blank control, none of the Daphnia were immobilised (see table below).
Table Immobility
Nominal Concentration in mg/L |
Immobility 24 hours |
Immobility 48 hours |
||||||||
absolute |
in % |
absolute |
in % |
|||||||
Blank control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
18 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
32 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
56 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
pH and O2
The pH values in the test media and the blank control and the concentration of dissolved oxygen are given in the following tables:
Table pH and O2-values
Nominal Concentration in mg/L |
pH |
O2-Concentration in mg/L |
||
0 h |
48 h |
0 h |
48 h |
|
Blank control |
7.6 |
7.7 |
9.2 |
8.5 |
10 |
7.6 |
7.7 |
9.2 |
8.6 |
18 |
7.6 |
7.7 |
9.3 |
8.7 |
32 |
7.6 |
7.7 |
9.3 |
8.8 |
56 |
7.6 |
7.7 |
9.2 |
8.7 |
100 |
7.6 |
7.7 |
9.3 |
8.7 |
Analytical Determinations
The measured concentrations were in the range between 94% and 107 % of the nominal concentration at the start and at the end of the test. Therefore, the determination of the biological results was based on the nominal concentration.
The measured concentrations for treatment and control are given in the following tables:
Table Measured Concentrations
Nominal Concentration |
Measured Concentration Test Item |
Measured Concentration Test Item t = 48 h |
mg/L |
mg/L |
mg/L |
Blank control |
< LOQ |
< LOQ |
10 |
9.38 |
10.05 |
18 |
17.27 |
18.82 |
32 |
31.03 |
33.49 |
56 |
55.62 |
59.76 |
100 |
96.87 |
106.52 |
LOQ (Limit of quantification) = 0.05 mg/L
Table Percentage of Nominal Concentrations and Recovery
Nominal Concentration Test Item |
% of Nominal concentration |
% of Nominal concentration |
mg/L |
% |
% |
Blank control |
-- |
-- |
10 |
94 |
101 |
18 |
96 |
105 |
32 |
97 |
105 |
56 |
99 |
107 |
100 |
97 |
107 |
Results and Statistics
Biological Results Test Item
The biological results are presented in the following table:
Table Biological Results Test Item
Parameter |
Value |
95% confidence interval |
24h EC50 |
> 100 mg/L |
not determinable |
48h EC50 |
> 100 mg/L |
not determinable |
48h NOEC |
≥ 100 mg/L |
not determinable |
48h LOEC |
> 100 mg/L |
not determinable |
Validity
The 24h-EC50
of K2Cr2O7 should lie between 0.6 and
2.1 mg/L.
The 24h-EC50 of K2Cr2O7 was determined
as 1.6 mg/Lin a separate
GLP study.
Immobilisation
in the controls may not exceed 10 %.
Immobilisation in the controls was 0 %.
The concentration of dissolved oxygen at the end of the test must be at least 3 mg/L. The lowest concentration of dissolved oxygen at the end of the test was 8.5 mg/L.
The pH-value
in the test solutions should not vary by more than 1.5 units during the
test.
The
highest variation was 0.1 units.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study was conducted under GLP according to OECD guideline 202 and EU method C.2 on the registered substance itself. The method is to be considered scientifically reasonable with no deficiencies in documentation or deviations from the guidelines, the validity criteria were met. Hence, the results can be considered as reliable to assess the toxicity of DPS towards daphnids.
The toxicity against Daphnia magna was tested in a static test design using several concentrations up to 100 mg/L. The concentrations showed no toxicity. None of the animals were immobilised neither in the treatments nor in the control.
Based on the obtained results, sodium 3-[[(dimethylamino)thioxomethyl]thio]propanesulphonate does not need to be classified as hazardous to the aquatic environment according to the Regulation (EC) No. 1272/2008. - Executive summary:
The 48–hr-acute toxicity of sodium 3-[[(dimethylamino)thioxomethyl]thio]propanesulphonate to Daphnia magna STRAUS Berlin was studied under static conditions. Daphnids were exposed to control, positive control Potassium dichromate, and test chemical at nominal concentrations of 0, 10, 18, 32, 56, and 100 mg/L for 48 hr. Immobilization was observed daily. The 48 – hour EC50 was >100 mg/L. The 48 – hr NOEC based on immobilization was ≥ 100 mg/L. The concentration showed no toxicity. None of the animals were immobilised neither in the treatment nor in the control.
Based on the results of this study, sodium 3-[[(dimethylamino)thioxomethyl]thio]propanesulphonate would not need to be classified as toxic to daphnids, i.e. hazardous to the aquatic environment in accordance with the classification system of Regulation (EC) No. 1272/2008.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
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