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EC number: 240-357-1 | CAS number: 16245-77-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of read across chemicals
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: As mention in below principle
- Principles of method if other than guideline:
- WoE report is prepared based on toxicity to microorganisms study including WoE 2, 3 and 4.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (IUPAC name): Benzene-1,4-diammonium sulphate- Molecular formula: C6H8N2.H2O4S- Molecular weight: 206.221 g/mol- Smiles notation: S(=O)(=O)(O)O.c1(ccc(cc1)N)N- InChI: 1S/C6H8N2.H2O4S/c7-5-1-2-6(8)4-3-5;1-5(2,3)4/h1-4H,7-8H2;(H2,1,2,3,4)- Substance type: Organic - Physical state: Solid
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Remarks:
- 1, 2nd study DMSO
- Details on test solutions:
- 1,2: - Method: Stock solutions of each were formulated by dissolving the chemical at concentrations of 5, 10, 50, or 100 ug/ml in dimethyl sulfoxide (DMSO) or sterile distilled water.
- Test organisms (species):
- other: 1, 2nd study: Tetrahymena pyriformis. 3rd study Ruminococcus albus and Butyrivibrio fibrisolvens
- Details on inoculum:
- Axenic cultures of T. pyriformis reared in a semidefined protease peptone medium were grown at 28°C under a static regime.
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 60 h
- Test temperature:
- 1, 2: 28 °C
- Details on test conditions:
- Details on test conditionsTEST SYSTEM- Test vessel: Erlenmeyer flask- Material, size, headspace, fill volume:250 ml Erlenmeyer flask containing 50 ml of test solution- No. of vessels per concentration (replicates): 2- No. of vessels per control (replicates): 3 EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Tetrahymena pyriformis was used as the bioassay system. Population growth and density being selected on which toxicity were measured.
- Reference substance (positive control):
- yes
- Remarks:
- 1, 2 study: 2-ketoalkane
- Key result
- Duration:
- 60 h
- Dose descriptor:
- other: IGC50
- Effect conc.:
- 10.38 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 95% CI was 4.52 – 13.91 mg/l
- Duration:
- 60 h
- Dose descriptor:
- other: IGC50
- Effect conc.:
- 7.35 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 95% CI was 5.95 – 8.98 mg/l
- Dose descriptor:
- other: IGC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Growth inhibition were observed in Ruminococcus albus
- Reported statistics and error estimates:
- 1,2: Probit analysis
- Validity criteria fulfilled:
- not specified
- Conclusions:
- 1. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 10.38 mg/l.2. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 7.35 mg/l.3. As the two different species Ruminococcus albus and Butyrivibrio fibrisolvens exposed with the test chemical, effect on the growth of organisms vary. The IGC for Ruminococcus albus was determine to be 10 mg/l and on the other species it was observed that the growth inhibited at 100 mg/l. Thus based on the overall studies for Benzene-1,4-diammonium sulphate(CAS No. 16245 -77 -5), toxicity value ranges from 10 mg/l to 10.38 mg/l.
- Executive summary:
Toxicity of Benzene-1,4-diammonium sulphate (CAS No. 16245 -77 -5) on the growth and other biological activity of microorganisms is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:
In the first study toxicity was measured.
Study was conducted to investigate the effect of test chemical on the growth of ciliate Tetrahymena pyriformis. Test conducted under the static system for 60 hrs. Chemical analytically monetarized by using HPLC. DMSO used as a solvent. Axenic cultures of T. pyriformis reared in a semidefined protease peptone medium were grown at 28°C under a static regime. 250 ml Erlenmeyer flask containing 50 ml of test solution. Duplicate assembly were arrange for the test. Tetrahymena pyriformis was used as the bioassay system. Population growth and density being selected on which toxicity were measured. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 10.38 mg/l.
Similarly aim of this study was to investigate the effect of test chemical on the growth of ciliate Tetrahymena pyriformis. Test conducted under the static system for 60 hrs. Chemical analytically monetarized by using HPLC. DMSO used as a solvent. Axenic cultures of T. pyriformis reared in a semidefined protease peptone medium were grown at 28°C under a static regime. 250 ml Erlenmeyer flask containing 50 ml of test solution. Duplicate assembly were arrange for the test. Tetrahymena pyriformis was used as the bioassay system. Population growth and density being selected on which toxicity were measured. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 7.35 mg/l with 95% CI was 5.95 – 8.98 mg/l.
In third study toxicity were observed at 10 mg/l. Aim of this study was to determine the toxicity of test chemical on the growth of microorganisms. Test conducted on two different organisms Ruminococcus albus and Butyrivibrio fibrisolvens. As the two different species Ruminococcus albus and Butyrivibrio fibrisolvens exposed with the test chemical, effect on the growth of organisms vary. The IGC for Ruminococcus albus was determine to be 10 mg/l and on the other species it was observed that the growth inhibited at 100 mg/l.
Thus based on the overall studies for Benzene-1,4-diammonium sulphate(CAS No. 16245 -77 -5), toxicity value ranges from 10 mg/l to 10.38 mg/l.
Reference
Description of key information
1. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 10.38 mg/l.
2. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 7.35 mg/l.
3. As the two different species Ruminococcus albus and Butyrivibrio fibrisolvens exposed with the test chemical, effect on the growth of organisms vary. The IGC for Ruminococcus albus was determine to be 10 mg/l and on the other species it was observed that the growth inhibited at 100 mg/l.
Thus based on the overall studies for Benzene-1,4-diammonium sulphate(CAS No. 16245 -77 -5), toxicity value ranges from 10 mg/l to 10.38 mg/l.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10.38 mg/L
Additional information
Toxicity of Benzene-1,4-diammonium sulphate (CAS No. 16245 -77 -5) on the growth and other biological activity of microorganisms is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:
In the first study toxicity was measured. Study was conducted to investigate the effect of test chemical on the growth of ciliate Tetrahymena pyriformis. Test conducted under the static system for 60 hrs. Chemical analytically monetarized by using HPLC. DMSO used as a solvent. Axenic cultures of T. pyriformis reared in a semidefined protease peptone medium were grown at 28°C under a static regime. 250 ml Erlenmeyer flask containing 50 ml of test solution. Duplicate assembly were arrange for the test. Tetrahymena pyriformis was used as the bioassay system. Population growth and density being selected on which toxicity were measured. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 10.38 mg/l.
First study was supported by the second experimental study from peer reviewed journal. Aim of this study was to investigate the effect of test chemical on the growth of ciliate Tetrahymena pyriformis. Test conducted under the static system for 60 hrs. Chemical analytically monetarized by using HPLC. DMSO used as a solvent. Axenic cultures of T. pyriformis reared in a semidefined protease peptone medium were grown at 28°C under a static regime. 250 ml Erlenmeyer flask containing 50 ml of test solution. Duplicate assembly were arrange for the test. Tetrahymena pyriformis was used as the bioassay system. Population growth and density being selected on which toxicity were measured. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 7.35 mg/l with 95% CI was 5.95 – 8.98 mg/l.
In third study toxicity were observed at 10 mg/l. Aim of this study was to determine the toxicity of test chemical on the growth of microorganisms. Test conducted on two different organisms Ruminococcus albus and Butyrivibrio fibrisolvens. As the two different species Ruminococcus albus and Butyrivibrio fibrisolvens exposed with the test chemical, effect on the growth of organisms vary. The IGC for Ruminococcus albus was determine to be 10 mg/l and on the other species it was observed that the growth inhibited at 100 mg/l.
Thus based on the overall studies for Benzene-1,4-diammonium sulphate(CAS No. 16245 -77 -5), toxicity value ranges from 10 mg/l to 10.38 mg/l.
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