Dicarbonyl(pentane-2,4-dionato-O,O')rhodium

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not reported

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

no guideline followed

Principles of method if other than guideline:

As JIS K0102 -55

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test item dissolved in 10 times its weight of a 1:1 mixture of acetone and Nikkol HCO40. Solution was then dispersed into the vessels to provide the test concentrations
- Controls: Solvent control included
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone and Nikkol HCO40

Test organisms (species):

Oryzias latipes

Details on test organisms:

Test organisms (species): Oryzias latipes
Details on test organisms - source: Wholesale Tropicals Ltd. London
Details on test organisms - holding conditions prior to test: Held for 14 days prior to test at 22 + 1⁰C in dechlorinated tap water
Details on test organisms - life stage of test species used: Mean length 25.4 mm, mean weight 1.7g

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

307 mg/l

Test temperature:

21 - 22°C

pH:

7.8 (dilution water)

Dissolved oxygen:

7.8 - 8.4 mg/L

Nominal and measured concentrations:

nominal concentrations: 10, 20, 30 40, 60 and 80 mg/L were tested

Details on test conditions:

test vessel 10L glass aquaria
aeration gentle aeration via narrow-bore glass tubes
no of organisms per vessel 10
no of replicates per concentration Seven groups, six test concentrations and a control group which was exposed to 800 mg/l of HCO40/acetone
photoperiod 16 /8 light/dark
TEST SYSTEM
- Test vessel: Glass aquaria holidng 10L test solution
- Aeration: Gentle aeration via narrow-bore glass tubes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: See below
- Total organic carbon: 6.6 mg/L
- Alkalinity: 175 mg/L as CaCO3
- pH: 7.8


OTHER TEST CONDITIONS
- Photoperiod: 16h light:8h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality recorded after 24 and 48 hours

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

19 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Confidence limits: 16 - 21 mg/L

Details on results:

Results calculated using method of Litchfield and Wilcoxon 1949. J. Pharmac.exp.Ther. 96 99-113

Validity criteria fulfilled:

not specified

Conclusions:

The 48 h LC50 for dicarbonyl(pentane-2,4-dionato-O,O2)rhodium is 19 mg/L.

Executive summary:

Ross and Pell (1981) is a non-guideline, non-GLP study. Solvents were used for preparation of the test solutions and a solvent control was included. Only a 48-hour exposure period was used. The study meets some OECD guideline 203 criteria but there are some limitations in the study design and there was no analytical verification of test concentrations and it is therefore assigned a Klimisch score of 2 (reliable with restircitions). However, despite the limitations it is considered suitable for use for assessing this endpoint. The 48 h LC50 of dicarbonyl(pentane-2,4 -dionato-O,O2)rhodium is 19 mg/L.

Description of key information

The 48h LC50 of dicarbonyl(pentane-2,4-dionato-O,O2)rhodium is 19 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

19 mg/L

Additional information

Ross and Pell (1981) is a non-guideline, non-GLP study. Solvents were used for preparation of the test solutions and a solvent control was included. Only a 48-hour exposure period was used. The study meets some OECD guideline 203 criteria but there are some limitations in the study design and there was no analytical verification of test concentrations. It is therefore assigned a Klimisch score of 2 (reliable with restrictions). Despite the study limitations the study is considered suitable for use for assessing this endpoint. The 48h LC50 of dicarbonyl(pentane-2,4 -dionato-O,O2)rhodium is 19 mg/L.