Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 224-929-8 | CAS number: 4559-86-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16.07.2012 to 18.07.2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- (2010)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- SGS INSTITUT FRESENIUS GmbH, Im Maisel 14, 65232 Taunusstein (Germany)
Test material
- Reference substance name:
- Tetrabutylurea
- EC Number:
- 224-929-8
- EC Name:
- Tetrabutylurea
- Cas Number:
- 4559-86-8
- Molecular formula:
- C17H36N2O
- IUPAC Name:
- 1,1,3,3-tetrabutylurea
- Reference substance name:
- 1,1,3,3-Tetrabutyl urea
- IUPAC Name:
- 1,1,3,3-Tetrabutyl urea
- Details on test material:
- - Name of test material (as cited in study report): Tetrabutyl urea, 1,1,3,3-Tetrabutyl urea
- Substance type: amide
- Physical state: colourless to yellowish liquid
- Analytical purity: 99.6%
- Impurities (identity and concentrations): dibutylamine: 0.04%; water: 0.04%, Cl: 0.8 ppm; S: < 1 ppm
- Purity test date: 2012-05-14
- Lot/batch No.: 2011-11-4
- Expiration date of the lot/batch: 2013-11-03
- Storage condition of test material: test substance stored dry and in tight closed container at 15-25°C in the dark
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Due to the low water solubility, the test substance concentrations were introduced into the test vessels by direct weighing.
- Controls: performed
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no vehicle used
- Evidence of undissolved material (e.g. precipitate, surface film, etc): not reported
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- -Inoculum: Activated sludge was collected from the effluent of the aeration tank of the municipal sewage treatment plant at Taunusstein-Bleidenstadt (Germany) dealing predominantly domestic sewages.
- Laboratory culture: no
- Preparation of inoculum for exposure: Immediately after receipt in the laboratory the activated sludge was washed twice with mineral medium acc. to OECD 301 B and aerated thereafter until test start. 10 L of the washed sludge were fed with 500 mL of the synthetic sewage. The feeding procedure was repeated one day after receipt.
- Pretreatment: no
- Initial biomass concentration: 1.5 g/L dry mass in the test treatments
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 +/- 2°C
- pH:
- in the definitive test
at the beginning of incubation: 7.08-7.62 (in all test vessels)
after incubation: 7.75-8.30 (in all test vessels) - Nominal and measured concentrations:
- 62.5, 125, 250, 500, and 1000 mg/L nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass bottle
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass/250 mL/no/250 mL
- Aeration: yes
- Type of flow-through (e.g. peristaltic or proportional diluter): test conducted under static conditions
- No. of organisms per vessel: not applicable
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 6
- No. of vessels per autoxidation control: 1 (performed with the highest test concentration)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic sewage was prepared according to OECD Guideline 209 . Adjustment of pH was not necessary (the criterion of the guideline was fulfilled: pH of the solution: 7.14; according to guideline: pH 7.5 +/- 0.5). Tap water was used to prepare the test concentrations.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): inhibition of total respiration, heterotrophic respiration, and respiration due to nitrification
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 10, 100, and 1000 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5 Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: total respiration
- Duration:
- 3 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Duration:
- 3 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Results with reference substance (positive control):
- The EC50 of the reference item 3,5-Dichlorophenol for total respiration was in the required range of 2-25 mg/L (65% inhibition at 15 mg/L)
- Reported statistics and error estimates:
- Statistic evaluation of EC values was done using the commercially available computer program ToxRatPro Version 2.10
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a study conducted according to guideline, the respiration inhibition (total respiration, heterotrophic respiration, and respiration due to nitrification) of Tetrabutyl urea to activated sludge was found to be low (NOEC>=500 mg/L, LOEC>=1000 mg/L, and EC50>1000 mg/L).
- Executive summary:
The toxicity of Tetrabutyl urea to activated sludge was investigated in a study conducted according to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test) in the version dated 22 -Jul-2010. Activated sludge from the aeration tank of a municipal sewage treatment plant treating predominantly domestic waste was exposed to Tetrabutyl urea at different concentrations (range: 62.5 to 1000 mg/L nominal). After 3 h of incubation the inhibition of respiration was determined in comparison to a set of test solutions without any test substance. The NOEC values for inhibition of total respiration, heterotrophic respiration, and respiration due to nitrification were determined to be 500, >=1000, and 500 mg/L nominal, respectively. The corresponding LOEC and EC50 values were 1000, >1000, and 1000 mg/L (LOEC) and >1000 mg/L nominal (EC50 each), respectively.
The study is regarded as reliable without restriction and satisfies the requirements of the guideline.
Results Synopsis
Test organism: activated sludge
-inhibition of total respiration
NOEC: 500 mg/L
LOEC: 1000 mg/L
EC50: >1000 mg/L
-inhibition of heterotrophic respiration
NOEC: >=1000 mg/L
LOEC: >1000 mg/L
EC50: >1000 mg/L
-inhibition of the respiration due to nitrification
NOEC: 500 mg/L
LOEC: 1000 mg/L
EC50: >1000 mg/L (all values refer to nominal concentrations)
Endpoint(s) effected: respiration
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.