Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 207-059-3 | CAS number: 429-60-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental start date: 19 August 2019, Experimental completion date: 15 October 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Trimethoxy(3,3,3-trifluoropropyl)silane
- EC Number:
- 207-059-3
- EC Name:
- Trimethoxy(3,3,3-trifluoropropyl)silane
- Cas Number:
- 429-60-7
- Molecular formula:
- C6H13F3O3Si
- IUPAC Name:
- trimethoxy(3,3,3-trifluoropropyl)silane
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Identification: ST2092NM
Batch: 802496
Purity: 100%
Physical State/Appearance: Clear colorless liquid
Expiry Date: 13 March 2020
Storage Conditions: Room temperature in the dark
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic, non-adapted
- Details on inoculum:
- Test system:
A mixed population of sewage treatment micro-organisms was obtained on 30 September 2019 from the final effluent stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK, which treats predominantly domestic sewage.
Preparation of Inoculum:
The sample of effluent was filtered through coarse filter paper (first approximate 200 mL discarded) and maintained on aeration in a temperature controlled room at 21 ±1 °C prior to use. - Duration of test (contact time):
- 13 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Mineral Medium:
The mineral medium usedwas that recommended in the OECD Guidelines.
The deionized reverse osmosis water used for the preparation of the mineral medium and the mineral medium used for the test contained less than 1 mg/L Total Organic Carbon (TOC).
Test Item Preparation:
For the purpose of the test, due to the volatile nature of the test item, and following the recommendations of the International Standards Organisation (ISO 10634, 1995) the test item was added directly to the test vessels using a micro-pipettor.
An initial test was terminated after 21 days due to a significant increase in the biological oxygen demand (BOD) values in the inoculum control vessels to values of over 60 mg O2/L which is the limit when critical examination of the data was required. It was therefore considered justifiable to terminate the test due to a significant decrease in the biodegradation values being observed for some of the vessels.
A further test was performed whereby an aliquot of mineral medium (495 mL) was dispensed into each test vessel prior to an amount of test item (42.1 µL, equivalent to 50 mg of test item determined by preliminary weighings) being added. The pH values were then measured using a Hach HQ40d Flexi handheld meter prior to an aliquot of inoculum (5 mL) being dispensed into the test vessel to give the test concentration of 100 mg/L. Due to the volatile nature of the test item each vessel was placed on the respirometer within 1 minute after the addition of the test item.
The inoculum control vessels were prepared in a similar manner without the addition of test item.
A test concentration of 100 mg/L was selected for use in the study following the recommendations of the Test Guidelines.
Reference Item Preparation:
A reference item, aniline (C6H5NH2), was used to prepare the procedure control vessels. An initial stock solution of 1000 mg/L was prepared by dissolving a nominal amount of the reference item, which was freshly distilled (500 mg), directly in mineral medium (500 mL) with the aid of ultrasonication for approximately 20 minutes and allowed to cool to a temperature of approximately 21 °C. An aliquot (50 mL) of this stock solution was diluted with mineral medium (350 mL) prior to measurement of the pH value using a Hach HQ40d Flexi handheld meter and then addition of inoculum (5 mL) and adjusting to a final volume of 500 mL with mineral medium, to give the test concentration of 100 mg/L. The volumetric flask containing the stock solution was inverted several times to ensure homogeneity.
The pH of the reference item stock solution was 7.4. The pH value was measured using a Hach HQ40d Flexi handheld meter.
Toxicity Control:
A toxicity control, containing the test item and aniline, was prepared in order to assess any toxic effects of the test item on the sewage treatment micro-organisms used in the test.
An aliquot of mineral medium (445 mL) was dispensed into each test vessel with an aliquot (50 mL) of the 1000 mg/L aniline stock solution prior to the addition of an amount of test item (42.1 µL, equivalent to 50 mg of test item determined by preliminary weighings). The pH values were then measured using a Hach HQ40d Flexi handheld meter prior to an aliquot of inoculum (5 mL) being dispensed into the test vessel to give the test concentration of 100 mg test item/L and 100 mg aniline/L. Due to the volatile nature of the test item each vessel was placed on the respirometer within 1 minute after the addition of the test item.
Preparation of Test System:
The following test preparations were prepared and inoculated in 500 mL bottles:
a) Three replicate bottles containing inoculated mineral medium to act as the inoculum control.
b) Two replicate bottles containing inoculated mineral medium and the reference item, aniline, at a concentration of 100 mg/L to act as the procedure control.
c) Three replicate bottles containing test item and inoculated mineral medium at a concentration of 100 mg/L.
d) Two replicate bottles containing the test item at a concentration of 100 mg/L in inoculated mineral medium and the reference item, aniline, at a concentration of 100 mg/L to act as toxicity control vessels.
Additional replicate vessels were prepared and incubated in order that in the event of a leak in the test system a replicate vessel could be discarded without jeopardizing the integrity of the study. From those samples prepared, on Day 13, two inoculum controls, one procedure control, two test item and one toxicity control vessel were sampled for pH analysis. The other vessels were discarded.
In this instance, some of the additional replicate vessels had some erroneous results and were not reported (one inoculum control vessel and one test item vessel). The procedure control and toxicity control vessels did not show any erroneous results so the first vessel in the test series was reported.
All vessels were inoculated with the prepared inoculum at a rate of 1% v/v.
On Day 0, the test and reference items were added to the mineral medium. The pH of all vessels was measured using a Hach HQ40d Flexi handheld meter prior to the addition of the inoculum and the volume in the procedure control vessels being adjusted to 500 mL by the addition of mineral medium.
In order to confirm that the aniline stock solution was prepared correctly, a diluted 100 mg/L stock solution (in reverse osmosis water) was also sampled for TOC analysis.
The test was conducted in diffuse light at temperatures between 22 and 23°C.
Reference substance
- Reference substance:
- aniline
Results and discussion
- Test performance:
- The difference between extremes of replicate BOD values at the time the plateau was reached and at the end of the 10 Day window was less than 20% and therefore satisfied the validation criterion given in the OECD Test Guidelines.
The toxicity control attained 72% biodegradation after 13 days thereby confirming that the test item was not toxic to the sewage treatment micro organisms used in the test.
Total Organic Carbon Confirmation: Total Organic Carbon of the diluted aniline stock solution confirmed that it had been prepared correctly.
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 68
- Sampling time:
- 13 d
- Details on results:
- The test item attained 68% biodegradation after 13 days, calculated from the oxygen consumption values, and satisfied the 10 Day window validation criterion, whereby 60% biodegradation must be attained with 10 days of the biodegradation exceeding 10%. The test item can therefore be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301F.
BOD5 / COD results
- Results with reference substance:
- Aniline (procedure control) attained 83% biodegradation after 13 days with greater than 60% degradation being attained in a 10-day window.
Any other information on results incl. tables
Table 1 Biological Oxygen Demand Values
Day |
BOD (mg O2/L) |
|||||
Inoculum Control |
Procedure Control |
Test Item |
Toxicity Control |
|||
R1 |
R2 |
R1 |
R2 |
|||
0 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
1 |
0.04 |
0.00 |
0.12 |
0.34 |
0.42 |
0.34 |
2 |
0.74 |
0.00 |
1.04 |
12.38 |
9.58 |
10.92 |
3 |
1.70 |
0.00 |
31.82 |
49.78 |
47.14 |
48.82 |
4 |
2.66 |
0.00 |
86.72 |
59.36 |
50.60 |
66.44 |
5 |
3.74 |
0.46 |
138.40 |
65.80 |
59.76 |
118.92 |
6 |
4.66 |
1.70 |
185.80 |
69.56 |
64.26 |
166.40 |
7 |
5.84 |
3.08 |
215.62 |
71.92 |
67.88 |
212.00 |
8 |
6.84 |
4.28 |
227.62 |
73.76 |
70.52 |
254.48 |
9 |
7.16 |
5.04 |
236.86 |
74.92 |
72.14 |
271.02 |
10 |
8.24 |
6.50 |
246.86 |
76.80 |
73.98 |
283.56 |
11 |
9.70 |
7.50 |
255.18 |
78.30 |
75.34 |
294.18 |
12 |
11.00 |
9.08 |
262.60 |
80.56 |
77.22 |
301.72 |
13 |
11.78 |
10.02 |
268.46 |
82.44 |
78.66 |
308.04 |
R= Replicate
Table 2 Percentage Biodegradation Values
Day |
Biodegradation (%) |
||||
Procedure Control |
Test Item |
Toxicity Control |
|||
R1 |
R2 |
Mean |
|||
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
2 |
0 |
12 |
9 |
11 |
3 |
3 |
10 |
48 |
45 |
47 |
12 |
4 |
28 |
56 |
48 |
52 |
16 |
5 |
44 |
62 |
56 |
59 |
28 |
6 |
59 |
64 |
59 |
62 |
40 |
7 |
68 |
65 |
62 |
64 |
50 |
8 |
72 |
66 |
63 |
65 |
60 |
9 |
75 |
67 |
64 |
66 |
64 |
10 |
78 |
67 |
65 |
66 |
67 |
11 |
80 |
68 |
65 |
67 |
69 |
12 |
82 |
68 |
65 |
67 |
71 |
13 |
83 |
69 |
66 |
68 |
72 |
R= Replicate
Table 3 pH Values of the Test Preparationson Days 0 and 13
Test Vessel |
pH |
|
Day 0 |
Day 13 |
|
Inoculum ControlR1 |
7.4 |
7.7 |
Inoculum Control R2 |
7.4 |
7.7 |
Procedure Control |
7.4 |
7.9 |
Test Item R1 |
7.4 |
7.6 |
Test Item R2 |
7.4 |
7.6 |
Toxicity Control |
7.4 |
7.8 |
R = Replicate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item attained 68% biodegradation after 13 days and satisfied the 10 Day window validation criterion, whereby 60% biodegradation must be attained with 10 days of the biodegradation exceeding 10%. The test item can therefore be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301F.
- Executive summary:
Introduction:
The study was performed to assess the ready biodegradability of the test item in an aerobic aqueous media. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No. 301F, “Ready Biodegradability; Manometric Respirometry Test” referenced as method C.4-D of Commission Regulation (EC) No. 440/2008 and US EPA Fate, Transport, and Transformation Test Guidelines OCSPP 835.3110 (Paragraph (q)).
Methods:
The test item at a concentration of 100 mg/L was exposed to sewage treatment micro‑organisms with mineral medium in sealed culture vessels in diffuse light at temperatures of between 22 and 23ºC for 13 days. The study was terminated after 13 days as degradation of the test item had reached a plateau and the test item attained greater than 60% biodegradation within 10 days of the test item attaining 10% biodegradation.
The biodegradation of the test item was assessed by the measurement of daily oxygen consumption values for 13 days. Control solutions with inoculum and the reference item, aniline, and a toxicity control were used for validation purposes.
Results:
The test item attained 68% biodegradation after 13 days and satisfied the 10‑Day window validation criterion, whereby 60% biodegradation must be attained within 10 days of the biodegradation exceeding 10%. The test item can therefore be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301F.
Aniline (procedure control) attained83% biodegradation after 13 dayswith greater than 60% degradation being attained in a 10-Day window.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.