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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 December 2007 - 6 February 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study has been performed according to OECD guidelines and according to GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Algal Growth Inhibition Test stipulated in the "Testing Methods for New Chemical Items" (November 21, 2003; No. 1121002, MHLW; November 13, 2003, No. 2, METI; No. 031121002, MOE, Japan, Partial amendment November 20, 2006)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Details on test material:
- - Name of test material (as cited in study report): S-500
- Substance type: yellow powder
- Physical state: solid
- Lot/batch No.: MF1456-2345
- Stability under test conditions: stable (confirmed by IR)
- Storage condition of test material: dark storage place at room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L (nominal) and control
- Sampling method: At the start, the solution sampled from the container for preparation in each test level was used for the measurement. At the end of the exposure, equal volume of the test solution was taken out from the test vessels in each test level and mixed, and then the solution after removal of algae by centrifugation (10 min, 3000 rpm) was used for the measurement.
- Sample storage conditions before analysis: not applicable.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: mixing and stirring by magnetic stirrer for 48 h, followed by suction filtration with 0.4 µm glass fibre filter and 0.2 µm membrane filter.
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 100 mg/l (nominal)
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: old name: Selenastrum capricornutum
- Strain: ATCC 22662
- Source (laboratory, culture collection): originally from American Type Culture Collection (in 1995) and cultured in the laboratory.
- Age of inoculum (at test initiation): 3 days
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): same as test
- Any deformed or abnormal cells observed: not indicated
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- not applicable
Test conditions
- Hardness:
- not indicated
- Test temperature:
- 23.0 - 23.2 °C
- pH:
- 7.9 - 8.0
- Dissolved oxygen:
- not measured
- Salinity:
- not applicable
- Nominal and measured concentrations:
- 0 and 100 mg/l (nominal)
Measured concentrations: not determined, i.e. < 0.0069 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: sterilized 500 mL erlenmeyer flask with gas-permeable silicon rubber plug. Fill volume: 100 mL
- Aeration: no, but continuous shaking (approx. 100 rpm)
- Initial cells density: 10000 cells/mL
- Control end cells density: 630000 cells/mL (mean)
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes (OECD 201)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: sterile purified water
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 90-95 µE/m2/s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 0, 24, 48 and 72 h
- Determination of cell concentrations: particle counter
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study:
- Test concentrations: 10%, 32% and 100% of a 100 mg/L nominal stock solution.
- Results used to determine the conditions for the definitive study: no cell growth inhibition observed at any test concentration. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Effect concentration > solubility in medium
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no abnormalities in control and exposed cells.
- Any stimulation of growth found in any treatment: mean cell concentration in exposed cells after 72 h: 790000 cells/mL (SD=34000). Mean cell concentration in control cells after 72 h: 630000 cells/ml (SD=64000).
- Effect concentrations exceeding solubility of substance in test medium: no observed effects at levels above the solubility of the substance in test medium. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 0.73 mg/L (growth rate) - Reported statistics and error estimates:
- F test: to determine the homogeneity of variance for the data
Aspin-Welch t-test: to estimate the significant difference in comparison with control.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study was conducted as a limit test in order to confirm the effect on the test organisms at around the solubility of the test item in medium. As a result, the concentrations of the test item in the test solutions during the exposure were below the determination limit (<0.0069 mg/L), indicating that the concentrations of the dissolved test item in medium was not clarified. However, no adverse effect was found in the definitive study. Therefore, it was decided that the test item had no adverse effect on the test organisms at around the solubility in medium. The environmental conditions were within the suitable range, therefore, it is concluded that this study complied with the applied test guidelines.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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