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Diss Factsheets
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EC number: 200-453-6 | CAS number: 60-09-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Carcinogenicity
Administrative data
- Endpoint:
- carcinogenicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable publication which meets basic scientific principles
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Rat skin carcinogenesis by topical applications of some azo dyes
- Author:
- Fare G.
- Year:
- 1 966
- Bibliographic source:
- Cancer Res 26: pp.2406-8
- Reference Type:
- secondary source
- Title:
- 4-Aminoazobenzene. BUA-Stoffbericht 217
- Author:
- Beratergremium für Altstoffe (BUA).
- Year:
- 1 998
- Bibliographic source:
- BUA der Gesellschaft dt. Chemiker, S. Hirzel Wissenschaftliche Verlagsgesellschaft, Stuttgart.
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 451 (Carcinogenicity Studies)
- Deviations:
- yes
- Remarks:
- only one dose used, only one sex used, only 6 animals used
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 4-aminoazobenzene
- EC Number:
- 200-453-6
- EC Name:
- 4-aminoazobenzene
- Cas Number:
- 60-09-3
- Molecular formula:
- C12H11N3
- IUPAC Name:
- 4-[(E)-2-phenyldiazen-1-yl]aniline
- Details on test material:
- Aminoazobenzene: Recristallized prior to use
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Albino outbred from own breeding
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: own breeding
- Age at study initiation: 3 to 4 months old
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
Administration / exposure
- Route of administration:
- dermal
- Vehicle:
- acetone
- Details on exposure:
- TEST SITE
- Area of exposure: between scapulae
- % coverage: no data
- Type of wrap if used: no data
- Time intervals for shavings or clipplings: Hair was shaved every 2 months in the early stages but much more frequently when tumors began to appear.
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1 ml
- Concentration (if solution): 0.2% solution
- Constant volume or concentration used: yes
VEHICLE
- Amount(s) applied (volume or weight with unit): 1 ml
- Concentration (if solution): 100%
USE OF RESTRAINERS FOR PREVENTING INGESTION: no - Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- mean time of treatment 123 weeks
- Frequency of treatment:
- twice per week
- Post exposure period:
- no post exposure period
Doses / concentrations
- Remarks:
- Doses / Concentrations:
1 ml of 0.2% solution in aceton
Basis:
analytical conc.
- No. of animals per sex per dose:
- 6 male animals
- Control animals:
- yes, concurrent vehicle
- Positive control:
- no
Examinations
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: No data
DETAILED CLINICAL OBSERVATIONS: No data
DERMAL IRRITATION (if dermal study): Yes
BODY WEIGHT: No data
OPHTHALMOSCOPIC EXAMINATION: No
HAEMATOLOGY: No
CLINICAL CHEMISTRY: No
URINALYSIS: No
NEUROBEHAVIOURAL EXAMINATION: No - Sacrifice and pathology:
- GROSS PATHOLOGY: Yes
HISTOPATHOLOGY: Yes
Results and discussion
Results of examinations
- Details on results:
- Result (carcinogenicity): positive
Effect levels
- Dose descriptor:
- conc. level:
- Effect level:
- 0.2 other: %
- Sex:
- male
- Basis for effect level:
- other: Skin tumors
- Remarks on result:
- other: Effect type: carcinogenicity (migrated information)
Any other information on results incl. tables
6 male rats treated twice weekly with Aminoazobenzene:
Mean time 1st lesion noted: 97th week
Mean length of treatment: 123 weeks
Tumor incidence: 0/6 ear duct; 6/6 skin
Total yield of skin tumors in group, postmortem: 5 epidermoid cysts; 4 squamous carcinoma; 8 basal carcinoma; 2 anaplastic carcinoma; 1 squamous papiloma; 3 miscellaneous
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.