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EC number: 940-437-2 | CAS number: 926-50-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 June 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- soil/sewage sludge
- Radiolabelling:
- no
- Test temperature:
- 35 °C
- Details on study design: HPLC method:
- EQUIPMENT
- Apparatus: Perkin Elmer (series 200) HPLC system comprising:
- a high pressure pump (module N2910102, serial number: 291 N8051901A)
- an auto-sampler (module N2930100, serial number: 293N8052404A)
- a column compartment (module N2601581, serial number: OVHO802151130)
- a refractive index detector (series 200a, serial number 125829).
MOBILE PHASES
- Type: methanol / water 55/45 (v/v))
- Column: 250 mm x 4.6 mm, Nucleodur® 100-5 CN-RP (Macherey-Nagel) serial number: N10080027, batch 34518023
- Flow rate: 1.0 mL/min.
- Column temperature: 35 °C
- Injected volume: 100 µL
DETERMINATION OF DEAD TIME
- Method: Formamide is used as an unretained compound for the determination of the "dead time" t0
REFERENCE SUBSTANCES
- Identity: Formamide, 2-Nitrobenzamide, Methyl benzoate, Naphthalene, 1,2,3-Trichloro benzene, Phenanthrene, 4,4’-DDT
DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 10.0 mg in 25 mL methanol/water 55/45 v/v).
- Quantity of reference substances: Formamide (4 g/L), 2-Nitrobenzamide (0.2 g/L), Methyl benzoate (0.2 g/L), Naphthalene (0.05 g/L), 1,2,3-Trichloro benzene (0.2 g/L), Phenanthrene (0.1 g/L) and 4,4’-DDT (0.2 g/L)
REPETITIONS
- Number of determinations: 2
EVALUATION
- Calculation of capacity factors k': k = (tR/t0)-1:
- Calculation of retention times: linear regression parameters
- Determination of the log Koc value: log KOC = a * log k + b - Key result
- Type:
- log Koc
- Value:
- 1.94 dimensionless
- Temp.:
- 35 °C
- Matrix:
- soil/sewage sludge
- Details on results (HPLC method):
- - Retention times of reference substances used for calibration: Formamide (3.31), 2-Nitrobenzamide (3.63), Methyl benzoate (4.54), Naphthalene (6.06), 1,2,3-Trichloro benzene (6.58), Phenanthrene (8.72) and 4,4’-DDT (16.07)
- Details of fitted regression line (log k' vs. log Koc): log KOC = a * log k + b
- Average retention data for test substance: 4.177 - Validity criteria fulfilled:
- yes
- Conclusions:
- The chromatograms of test item show one peak at an average retention time of 4.177 minutes, corresponding to an adsorption coefficient (log value) of 1.94. Therefore: Adsorption coefficients (Koc) of test item is log Koc = 1.94.
- Executive summary:
The Adsorption coefficient (Koc) on soil and on sewage sludge of test substance was determined by the Reverse Phase High Performance Liquid Chromatographic Method according to the OECD Guideline 121.
After equilibration of the HPLC system, the calibration mixture was injected first, followed by the test chemical solution twice and the calibration mixture again. Retention times tR were measured and averaged and the decimal logarithms of the capacity factors k were calculated. The linear regression parameters of the relationship log Koc = f (log k) were also calculated from the data obtained with the calibration mixture and, therewith, log Koc of the test substance was determined from its measured capacity factor. Peak assignment was confirmed by injecting solutions of the reference substances individually.
The chromatograms of test substance pure show one peak at an average retention time of 4.177 minutes, corresponding to an adsorption coefficient (log value) of 1.94. Therefore, adsorption coefficients (Koc) of test substance log Koc = 1.94.
Reference
Table 5.4.1/1: retention times for reference and test substance and calculation of Adsorption coefficient
REFERENCE SUBSTANCES |
log Koc |
tR1 |
tR2 |
tRm |
k |
log k |
Formamide |
- |
3.301 |
3.317 |
3.31 |
- |
- |
2-Nitrobenzamide |
1.45 |
3.632 |
3.624 |
3.63 |
0.096 |
-1.016 |
Methyl benzoate |
1.80 |
4.543 |
4.535 |
4.54 |
0.372 |
-0.430 |
Naphthalene |
2.75 |
6.061 |
6.055 |
6.06 |
0.831 |
-0.081 |
1,2,3-Trichloro benzene |
3.16 |
6.583 |
6.585 |
6.58 |
0.990 |
-0.004 |
Phenanthrene |
4.09 |
8.726 |
8.719 |
8.72 |
1.636 |
0.214 |
4,4’-DDT |
5.63 |
16.049 |
16.097 |
16.07 |
3.857 |
0.586 |
Test item |
|
4.175 |
4.179 |
4.177 |
0.2623 |
-0.581 2 |
Description of key information
A study has been performed with the registered substance for the estimation of its Adsorption coefficient by the HPLC method (OECD 121).
The substance was found to have a log Koc of 1.94 (Koc : 87 L/Kg).
Key value for chemical safety assessment
- Koc at 20 °C:
- 87
Additional information
The result indicates that the substance has a high mobility in soils (according to P.J. McCall et al., 19811).
1McCall P.J., Laskowski D.A., Swann R.L., and Dishburger H.J., (1981), “Measurement of sorption coefficients of organic chemicals and their use, in environmental fate analysis”, in Test Protocols for Environmental Fate and Movement of Toxicants. Proceedings of AOAC Symposium, AOAC,
Washington DC.
Mobility classification scheme:
Range of Koc |
Mobility class |
0 - 50 |
Very high |
50 - 150 |
High |
150 - 500 |
Medium |
500 - 2000 |
Low |
2000 - 5000 |
Slightly |
> 5000 |
Immobile |
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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