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EC number: 291-768-8 | CAS number: 90480-35-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 2,6-Octadienal, 3,7-dimethyl-, acid-isomerized
- EC Number:
- 291-768-8
- EC Name:
- 2,6-Octadienal, 3,7-dimethyl-, acid-isomerized
- Cas Number:
- 90480-35-6
- IUPAC Name:
- 1-methyl-4-(propan-2-yl)benzene; ethanol
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- male
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Age at study initiation: young adults
- Housing: maximum 4 mice / cage; suspended cages (18.4cm x 52.3cm x 12.7cm)
- Diet: free access to Porton Combined Diet
- Water: ad libitum
- Acclimation period: minimum 4 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 +/- 2
- Humidity (%): 55 +/- 15
- Air changes (per hr): 20-30
- Photoperiod (hrs dark / hrs light): 12 / 12
Study design: in vivo (LLNA)
- Vehicle:
- other: acetone
- Concentration:
- 1, 10 and 30%
- No. of animals per dose:
- 4
- Details on study design:
- Pre-screen test: A range finding test was not conducted.
Main study
- approx. 25 µl of test preparation applied to the dorsum of each ear
- vehicle control group was similarly treated using acetone
- the procedure was repeated daily for 3 consecutive days
- three days after the third application, all the animals were injected 250 µl of phosphate buffered saline (PBS) containing approx. 20 µCi of a 2.0 Ci/mmol specific activity 3H-methylthymidine 3H(Tdr)
- approx. 5 hours later, the animals were humanely killed by inhalation of halothane vapour followed by cervical dislocation.Sacrifice
- Negative control: vehicle control
- Method: pooled treatment group approach
Evaluation criteria
- Criteria used to consider a positive response: 2 criteria:
a. The increase in isotope incorporation for at least one concentration tested must be 3-fold or more compared with the vehicle control.
b. The data generated must be compatible with a biological dose-response. - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
Results and discussion
- Positive control results:
- Hexylcinnamaldehyde has fulfilled the criteria for a potential sensitiser.
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Value:
- 0.8
- Test group / Remarks:
- 1%
- Parameter:
- SI
- Value:
- 2.78
- Test group / Remarks:
- 10%
- Key result
- Parameter:
- SI
- Value:
- 4.95
- Test group / Remarks:
- 30%
Applicant's summary and conclusion
- Interpretation of results:
- Category 1B (indication of skin sensitising potential) based on GHS criteria
- Conclusions:
- The substance is considered to have the capacity to cause contact skin sensitisation.
- Executive summary:
In the current study the skin sensitisation potential of the test item was assessed using the Local Lymph Node Assay (LLNA) according to the OECD TG and GLP.
The assay determines the level of T-lymphocyte proliferation in the lymph node draining the site of chemical application, by measuring the amount of radiolabelled thymidine incorporated into the dividing cells.
The concentrations tested were 1, 10 or 30% in acetone and 4 male mice were used per group. The pooled treatment group approach was applied.
Hexylcinnamaldehyde was taken along as a positive control in the study and showed the capacity to cause contact sensitisation when applied as 3 or 10%.
The increase in isotope incorporation was greater than 3-fold at a concentration of 30% (SI = 4.95). Also, the response was consistent with a biological dose-response.
Under the conditions of the test, the test item fulfilled the criteria for a potential sensitiser and therefore the test substance is considered to have the capacity to cause contact skin sensitisation.
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