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EC number: 291-768-8 | CAS number: 90480-35-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-05-07 to 1999-06-04
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- The study was conducted and documented according to OECD 301 D and EU Directive 92/69 Method C.4-E.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Remarks:
- secondary effluent
- Details on inoculum:
- - Source: secondary effluent of domestic sewage treatment plant (Kläranlage Odenthal)
- Pretreatment: 7 days aeration
- Concentration of sludge: 5 ml/L
- Water filtered: yes, to remove coarse particles - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2.9 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Initial concentration: 2.9 mg/L by direct weighing
- Test temperature: 20 +/- 1 °C
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: 2 replicates for each treatment
blank inoculum
reference substance at 2.9 mg/L
test substance at 2.9 mg/L
toxicity control at 2.9 mg/L
SAMPLING
- Sampling frequency: day 0, 7, 14, 21 and 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 2 replicates
- Abiotic sterile control: no
- Toxicity control: yes, 2 replicates
- Other: reference substance
- Reference substance:
- benzoic acid, sodium salt
- Test performance:
- No unusual observations
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 42
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- A closed bottle test was conducted to assess the biodegradability of the substance. A biodegradation of 42 % after 28 days incubation was observed. Thus, the pass level of 60% degradation was not met and the result of the test is not readily biodegradable. However, as the test item still degraded > 40% degradation in the Closed Bottle Test, it is a clear indication that extensive primary biodegradation has occured. Therefore the test substance is considered to be inherently biodegradable. The toxicity control shows that the test item is not inhibitory at a concentration of 2.9 mg/L.
- Executive summary:
The biodegradatability of the test substance was assessed according to OECD 301 D and EU Method C.4 -E (Directive 92/69), a closed bottle test.
The test item was suspended in a mineral medium by direct weighing, inoculated with a mixed population of aquatic microorganisms from the secondary effluent of a domestic sewage treatment plant and incubated for 28 days in the dark. The biodegradation of the test substance was assessed by measuring the reduction of dissolved oxygen.
The reference substance (2.9 mg/L sodium benzoate) showed a biodegradation of 80 % fulfilling the validity criteria within 14 days . Moreover, 56 % biodegradation was observed in the toxicity control (2.9 mg/L test substance + 2.9 mg/L reference substance), indicating that the test substance was not inhibitory to the inoculum.
Within the test period of 28 days, the biodegradation of the test substance was only 42 %. The pass levels were not reached by the end of the 28-d period. Thus, the result of the ready test is "not readily biodegradable". However, as > 40% degradation were observed in the ready biodegradability test, the test substance is considered to be inherently biodegradable.
Reference
Result of biodegradation study:
Substance | Replicate | % degradation after | |||
7d | 14d | 21d | 28d | ||
Test item | 1 | 21 | 27 | 37 | 43 |
2 | 26 | 29 | 36 | 41 | |
Mean | 23.5 | 28.0 | 36.5 | 42.0 | |
std dev | 2.5 | 1.0 | 0.5 | 1.0 | |
Reference substance | 1 | 68 | 80 | 84 | 86 |
2 | 68 | 80 | 85 | 85 | |
Mean | 68 | 80 | 85 | 86 | |
std dev | 0.0 | 0.0 | 0.7 | 0.7 | |
Toxicity control |
1 | 40 | 47 | 51 | 55 |
2 | 41 | 47 | 52 | 57 | |
Mean | 41 | 47 | 52 | 56 | |
std dev | 0.7 | 0.0 | 0.7 | 1.4 |
Within the 28 -day test period, a degradation of 42% was observed. Hence, the substance is not readily biodegradable.
The reference item, sodium benzoate, biodegraded to 80% within 14 days.
The results of the toxicity control show biodegradation up to 47 % within 14 days, hence, the test substance was not inhibitory to the inoculum at a test concentration of 2.9 mg/L.
Description of key information
The biodegradability of the test item was assessed in according to OECD 301 D and EU method C.4, in a closed bottle test. A biodegradation of 42 % after 28 days incubation was observed. The test result is "not readily biodegradable".
However, as > 40% degradation were observed in the ready biodegradability test, the test substance can be considered to be inherently biodegradable.
The toxicity control shows that the test item is not inhibitory at a concentration of 2.9 mg/L.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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