Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 May 2017 - 26 July 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- SOURCE:
The source of the test organisms was activated sludge freshly obtained from a municipal sewage treatment plant, receiving predominantly domestic sewage. / `s-Hertogenbosch, The Netherlands
PREPARATION OF INOCULUM FOR EXPOSURE:
The freshly obtained sludge was used immediately. The concentration of suspended solids was determined to be 3.9 g/L in the concentrated sludge.
Before use, the sludge was allowed to settle (37 minutes) and the supernatant liquid was used as inoculum at the amount of 10 mL/L of mineral medium - Duration of test (contact time):
- >= 28 d
- Initial conc.:
- >= 19 mg/L
- Based on:
- test mat.
- Initial conc.:
- >= 12 mg/L
- Based on:
- other:
- Remarks:
- TOC/L
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Test vessels: 2 liter brown coloured glass bottles
- Milli-RO water: Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon.
- Stock solutions of mineral components
A) 8.50 g KH2PO4
21.75 g K2HPO4
67.20 g Na2HPO4.12H2O
0.50 g NH4Cl
dissolved in Milli-RO water and made up to 1 litre,
pH 7.4 ± 0.2
B) 22.50 g MgSO4.7H2O dissolved in Milli-RO water
and made up to 1 litre.
C) 36.40 g CaCl2.2H2O dissolved in Milli-RO water and
made up to 1 litre.
D) 0.25 g FeCl3.6H2O dissolved in Milli-RO water and
made up to 1 litre.
- Mineral medium: 1 litre mineral medium contains: 10 ml of solution (A), 1 ml of solutions (B) to (D) and Milli-RO water.
- Barium hydroxide: 0.0125 M Ba(OH)2 (Boom, Meppel, The Netherlands), stored in a sealed vessel to prevent absorption of CO2 from the air.
- Illumination: The test media were excluded from light
- Test temperature: from 22.2 to 23.3°C
- pH: Start of the test - 7.6 (adjusted with HCl from 7.8); End of the test - 7.5-7.8
- Aeration of the mixture (mineral components, Milli-RO water, inoculum): Yes, with synthetic air, overnight before the start of the test
- Suspended solids concentration: 3.9 g/L
TEST SYSTEM
- Culturing apparatus: 2 litre all-glass brown coloured bottles.
- Number of culture flasks/concentration:
The study consisted of six bottles:
- 2 inoculum blanks (no test substance),
- 2 test bottles with the test substance, at a test concentration 19 mg/L,
- 1 positive control (sodium acetate) and
- 1 toxicity control (Crosslinker CX-100 plus sodium acetate).
- Test performed in open system: Yes
- Details of trap for CO2: CO2 was trapped in barium hydroxide solution.
- Method used to create aerobic conditions / Synthetic air (CO2 < 1 ppm): A mixture of oxygen (ca. 20%) and nitrogen (ca. 80%) was passed through a bottle, containing 0.5 - 1 litre 0.0125 M Ba(OH)2 solution to trap CO2 which might be present in small amounts. The synthetic air was sparged through the scrubbing solutions at a rate of approximately 1-2 bubbles per second (ca. 30-100 mL/min).
SAMPLING
- Sampling frequency
Day: 1, 4, 6, 8, 11, 15, 18, 22, 25, 29 (3 times) for the test substance and the blank.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: No
- Toxicity control: Yes
- Other: Positive control
- Details of trap for CO2 and volatile organics if used: Barium hydroxide 0.0125 M Ba(OH)2 - Reference substance:
- acetic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 1.1
- Sampling time:
- 29 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test substance was not readily biodegradable under the conditions of the modified Sturm test performed
- Executive summary:
The ready biodegradability of the test substance was assessed using the carbon dioxide (CO2) evolution test according to OECD guideline 301B.
The test item was tested in duplicate at a concentration of 19 mg/L, corresponding to 12 mg TOC/L, during 28 days. Based on the obtained results, the test substance was classified as not readily biodegradable.
The present study is classified as reliable without restrictions since all acceptability criteria stated in the guideline were met.
Reference
Comparison of Biodegradation of the Test Item in Bottles A and B
Day |
Biodegradation (%) |
|||
Bottle A |
Bottle B |
Mean A and B |
∆ A-B1) |
|
1 |
0 |
0 |
0 |
0 |
4 |
1 |
1 |
1 |
0 |
6 |
2 |
2 |
2 |
0 |
8 |
2 |
4 |
3 |
2 |
11 |
2 |
5 |
4 |
3 |
15 |
2 |
7 |
5 |
5 |
18 |
2 |
7 |
5 |
5 |
22 |
2 |
8 |
5 |
6 |
25 |
2 |
9 |
6 |
7 |
292) |
2 |
10 |
6 |
8 |
292) |
2 |
11 |
7 |
9 |
292) |
2 |
12 |
7 |
10 |
1): Absolute difference in biodegradation between bottles A and B 2): Biodegradation is ended on day 28 by addition of HCl. Therefore, differences observed on day 29 are actually differences of day 28. |
Description of key information
The objective was the evaluation of the biodegradability in water of the test substance.
The ready biodegradability of the test substance was assessed using the carbon dioxide (CO2) evolution test according to OECD guideline 301B.
Based on the obtained results, the test substance was classified as not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The ready biodegradability of the test substance was assessed using the carbon dioxide (CO2) evolution test (modified Sturm test) according to OECD guideline 301B. The source of test organism was activated sludge freshly obtained from a municipal sewage treatment plant. A single test concentration of 19 mg substance/L (corresponsing to 12 mg TOC/L) was tested during 28 days. The CO2 evolution was measured as parameter for biodegradation. Only 1.1% degradation was observed after 28 days. Based on the obtained results, the test substance was classified as not readily biodegradable.
The present study is classified as reliable without restrictions since all acceptability criteria stated in the guideline were met.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.