Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 249-636-2 | CAS number: 29450-45-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10.05.2004 - 31.05.2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Cross-reference
- Reason / purpose for cross-reference:
- read-across source
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (4-chloro-2-methylphenoxy)acetic acid
- EC Number:
- 202-360-6
- EC Name:
- (4-chloro-2-methylphenoxy)acetic acid
- Cas Number:
- 94-74-6
- Molecular formula:
- C9H9ClO3
- IUPAC Name:
- (4-chloro-2-methylphenoxy)acetic acid
- Reference substance name:
- Water
- EC Number:
- 231-791-2
- EC Name:
- Water
- Cas Number:
- 7732-18-5
- Molecular formula:
- H2O
- IUPAC Name:
- water
- Reference substance name:
- 4-chloro-o-cresol
- EC Number:
- 216-381-3
- EC Name:
- 4-chloro-o-cresol
- Cas Number:
- 1570-64-5
- Molecular formula:
- C7H7ClO
- IUPAC Name:
- 4-chloro-2-methylphenol
- Test material form:
- solid
Constituent 1
impurity 1
impurity 2
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
Any other information on results incl. tables
Results and conclusion
Every validation criteria was met:
· mortality of the parent animals did not exceed 20% at the end of the test; and
· the mean number of alive offspring produced per parent animals at the end of the test was >= 60.
There was no significant effect ofMCPA tech.to survival of parent animal. There was 20% mortality (two parent animal) that occurred on day 14thand 16thin control and also in concentration 10 mg/1 (18thday of test), 56 mg/1 (14thand 16thday of test) and 100 mg/1 (9thday of test). These mortalities however were statistically not significant as verified by Fisher's Exact Test. There were also dead young animals (offspring) found; one in control in 16thday of test, and in concentration 100 mg/1, in total, thirty between 7thand last day of test.
Total number of living offspring per parent animal (for each replicate) alive at the end of the test versus concentration of the test substance is presented on in Table 6
Table 6 Daphnia magna reproduction test – sum up of offspring of parent animals in all concentrations of MCPA tech. and control.
Table 6. Daphnia magna reproduction test — sum up of offspring of parent animals in all concentrations of MCPA tech. and control.
Replicate No |
Number of live offspring after 21 days in concentrations |
|||||
Control |
10 mg/l |
18 mg/l |
32 mg/l |
56 mg/l |
100 mg/l |
|
|
||||||
1 |
178 |
173 |
159 |
152 |
153 |
118 |
2 |
82 |
89 |
166 |
144 |
196 |
53(12) |
3 |
1(1) |
* |
158 |
79 |
127 |
85 |
4 |
162 |
59 |
185 |
161 |
* |
79(6) |
5 |
117 |
134 |
65 |
65 |
125 |
69(8) |
6 |
145 |
143 |
159 |
70 |
177 |
82 |
7 |
141 |
84 |
|
156 |
* |
* |
8 |
* |
* |
102 |
153 |
187 |
96(3) |
9 |
124 |
151 |
141 |
143 |
176 |
44 |
10 |
* |
59 |
141 |
121 |
* |
92(1) |
|
||||||
Total |
950 |
892 |
1276 |
1 244 |
1 141 |
718 |
Mean |
118.75 |
111.5 |
141.7 |
124.4 |
163 |
79.7 |
Variance |
3 115.93 |
1 945.14 |
1 348.7 |
1 467.4 |
811,7 |
502.4 |
t value |
|
|
|
|
|
|
* parent animal died
( ) In brackets number of dead young animals.
Coefficient of variation for reproduction in control (based on total number of living offspring per living parent animal) = 3,8 %.
The Kolmogorov "D" Test for normality assumption of data distribution generated D+value of 0.0817 and D-value of 0.1391. The final D* value = 1.0083 is lower than critical one (i.e. 1,035 at p0,01). Therefore the conclusion of the Kolmogorov"D"Test is that data are normally distributed. Bartlett's Test used to estimate if variance is homogeneous or heterogeneous generated B value of 7.313. That is less than the critical one (15.086). Conclusion is that variance is homogeneous.
T-Test with Bonferroni's Adjustment was performed to determine NOEC value. This test showed that all obtained t values for the consecutive concentrations were below the critical value (critical value is 2.404 at p0.05). It means that there is no statistical significant difference in reproduction between control and consecutive concentrations. The statistical values for each concentration are also given in Table 6.
In conclusion, taking into account the obtained results as well as statistical analysis the NOEC for MCPA tech. is above 100 mg/l measured concentration while LOEC also is above 100 mg/l measured concentration.
Applicant's summary and conclusion
- Executive summary:
Daphnia magna (Crustacea, Cladocera) reproduction test was performed according to the OECD Guideline No 211 (adopted September 21, 1998) and Principles of Good Laboratory Practice (GLP, OECD 1997).
The aim of the study was determination of reproductive output of Daphnia magna exposed to the range of concentrations of MCPA technical (determination of NOEC after 21-day exposure). The test preparation was used in 5 concentrations in the range from 10 to 100 mg/l with separation factor of 1,8. Because the statistical analysis shown no significant differences between control and concentrations in reproduction levels hence the NOEC (21-day in mg/l) is above 100 mg/l while LOEC is also above 100 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.