Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 276-333-2 | CAS number: 72089-08-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 Oct - 09 Nov 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 17 Jul 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Staatliches Gewerbeaufsichtsamt Hildesheim, Germany
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant, Hildesheim, Germany (04 Oct 2016)
- Storage conditions: In aerobic conditions by aeration with CO2-free air.
- Storage length: 7 d
- Pretreatment: The activated sludge was washed twice with chlorine-free tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 3 h. Thereafter, the sludge was homogenized with a blender. After sedimentaiton the supernatant was decanted and maintained in a aerobic conditions by aeration with CO2-free air for 7 d.
- Concentration of inoculum: 10 mL/L were used to initiate inoculation (1.11E+09 colony forming units/L)
- Initial cell/biomass concentration: 1.11E+07 colony forming units/L (final test solution) - Duration of test (contact time):
- 28 d
- Initial conc.:
- 19.9 mg/L
- Based on:
- test mat.
- Initial conc.:
- 60 mg/L
- Based on:
- ThOD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium stock solutions diluted in ultrapure water, according to guideline.
- Test temperature: 20.4 - 21.0 °C
- pH: 7.70 - 7.73 at test start and 7.70 - 7.96 (at test end)
- pH adjusted: No
- Continuous darkness: Yes
TEST SYSTEM
- Culturing apparatus: Brown glass bottles (500 mL)
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Aeration with CO2-free air
- Measuring equipment: OxiTop measuring heads
- Test performed in open system: No
- Details of trap for CO2: A rubber sleeve with soda lime was hung into the opening of the bottles to absorb evolved CO2.
- Other: The test solutions were continuously stirred during the test.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes, 250 mL test medium (mineral medium stock solutions and inoculum in ultrapure water) without test and/or reference item (2 replicates).
- Abiotic sterile control: No.
- Toxicity control: Yes, reference item and test item in 250 mL reference item medium (reference item and inoculum in mineral medium stock solutions and ultrapure water) (1 replicate).
- Functional control: 250 mL reference item medium (reference item and inoculum in mineral medium stock solutions and ultrapure water) (1 replicate). - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 4
- Sampling time:
- 28 d
- Remarks on result:
- other: mean value
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item is classified as not readily biodegradable within the 28 d period of the study.
The degradation of the reference item was not inhibited by the test item. - Executive summary:
The ready biodegradability of the test item was determined with non-adapted activated sludge in a manometric respirometry test for a period of 28 d according to OECD guideline 301 F. The test item concentration was 19.9 mg/L (60.0 mg O2/L). Both test item replicates did not reach the 10% level (begin biodegradation phase) within the 28 d period. The mean biodegradation on Day 28 was 4%.
Reference
The biodegradation in % was calculated based on a ThOD of 3.02 mg O2/mg test item.
FUNCTIONAL CONTROL
The pass level for ready biodegradability (> 60%) was reached on Day 3. The validity criterion that the degradation should be > 60% after 14 d was fulfilled.
TOXICITY CONTROL
In the toxicity control the biodegradation was 47% after 14 d. After 28 d the biodegradation reached 57%. The validity criterion that the test item should not inhibit the biodegradation of the reference item was fulfilled.
Description of key information
Not readily biodegradable: 4% in 28 d (activated sludge, domestic, non-adapted, OECD 301 F)
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
There is one GLP study available investigating the ready biodegradability of the test substance according to the OECD guideline 301 F.
In a Manometric Respirometry Test, a test medium with 19.9 mg/L test item (nominal, 60.0 mg O2/L) was inoculated with 1.11E+07 colony forming units/L non-adapted activated sludge from a local, municipal sewage treatment plant and incubated for 28 d. A functional, inoculum, as well as a toxicity control were run in parallel. Oxygen depletion by the respiration of bacteria was monitored using the OxiTop system. The percentage biodegradation was expressed in terms of biological oxygen demand (BOD) and theoretical oxygen demand (ThOD).
The functional control reached the pass level (> 60%) on Day 3 and maximum biodegradation (100%) on Day 25. In the toxicity control 47% degradation occurred by Day 14 and 57% by Day 28. Therefore, the degradation of the reference item was not inhibited by the test item.
Both replicates of the test item did not reach the 10% level (end of adaptation, begin of biodegradation phase) within the 28 d period of the test. The mean biodegradation of the test substance on Day 28 was 4%. Hence, the test item is classified as not readily biodegradable within the 28 d period of the study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.